The experiment aims to study the effect of substrate concentration on the activity of polyphenol oxidase (PPO) from banana extracts using spectrophotometric techniques.
An enzyme refers to a molecule that is protein in nature and works as a biological catalyst. The enzyme possesses three major characteristics. The first characteristic is that they act to increase the rate at which a given reaction takes place. This enables most cellular reactions to take place at up to million times faster compared to their rate in the absence of an enzyme. Secondly, most enzymes act specifically on a single reactant known as ...