Protein Reports Samples For Students

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs.
Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

Aim: To separate Green Fluorescent Protein (GFP) using poly-acrylamide gel electrophoresis (PAGE).

Introduction: Bioluminescence observed in many marine organisms is due to a protein called as Green Fluorescent Protein (GFP). Since its discovery, GFP has been useful in biotechnology due to its versatility and ease of transformation in E. coli (Chalfie et al., 1994). GFP is a 26.87 kilodaltons (kD) protein composed of 239 amino acids. It has a barrel structure with a fluorescent chromophore embedded deep in the interior of the protein. The chromophores in GFP are excited at a wavelength of 395 nm, and when the electrons of chromophores drop to a lower energy state they emit fluorescent light of 509 nm (Ormo et al., ...

Question 2: How many serves of each of the five food groups and discretionary foods did you have on average across your two days? Comment on how this intake compares to the recommended serves listed in the Australian Dietary Guidelines appropriate to your age and gender? Which foods contributed most to your intake, or were missing from your intake if you ate minimal serves from a particular food group? (600 words)
Across the two days, each of the food category was only served once with an exception of whole meal bread (carbohydrate and dietary fiber) and chicken-meat (hem protein), ...

Introduction

In the mouse and other mammalian species, the pool of primordial follicles becomes established during fetal or neonatal life (Oktay et al., 1995). Over the mammal’s lifespan, the follicles undergo growth and development, and eventually the initial reserve of follicles begins to diminish during the mammal’s midlife. Briefly, follicular cells move from a primordial phase, in which little DNA synthesis is taking place, to enter a primary and then secondary stage, in which the granulose cells undergo intense proliferation and high levels of DNA synthesis can be observed. Antral and post- ovulation stages follow (Oktay et al., 1995). Folliculogenesis ...

Purpose

The experiment aims to identify two unknown amino acids using paper chromatography. To be specific, the retention factor or Rf values of standard amino acids was used to see which one matched that of the unknowns.

Introduction

Chromatography can be defined as the separation of a mixture into various fractions by distribution between a mobile phase, which can be a gas or a liquid, and a stationary phase, a liquid or solid. Different substances have different affinities for the stationary and mobile phases. A substance that has no affinity for the stationary phase will remain in the mobile phase and move rapidly through the chromatographic system. ...

Introduction

Mutation induces the formation mRNA strands that are formed later that they are converted into a native protein. Mutational causes the peptide formed develop a structure similar to a protein. Antimicrobial peptide formed after mutation depends on the chemical property. Mutation causes bacteria to increase in the peptide leading to low hemolytic function. Bacteria lead to anoplin to causing the shape of peptide to form a protein like shape that is spectroscopy.
Mutation causes the chain of the peptide to form a protein structure that later forms a tertiary that is the antigen molecule responsible for forming the body immune system. The chains amino ...

Biology

The electron microscope has proven to be invaluable in the study of proteins and has enhanced our understanding of cellular biology. The importance to understanding disease and trauma to the body as well as treatments cannot be overstated. Genetic research such as studies of the mechanisms of protein folding is better understood due to electron microscopy.
The image of a sample such as a slice of a fibril or a “newly synthesized chain of amino acids” (Dobson, 2003, p. 884) created by an electronic beam. The Transmitting Electron Microscope (TEM) transmits the electron beam through a sample whereas the SEM scans the surface ...

- Abstract
Cardiovascular diseases such as heart failure are some of the leading causes of mortality and morbidity with Australia being one of the country’s most greatly affected (McLean, Esclick & Coats, 2006). An area of interest in the pathology of heart failure is the significance that polymorphisms of adrenergic receptor genes have on the outcomes of patients with heart failure. Single-nucleotide polymorphisms or SNPs that occur in the genes that encode for adrenergic receptors are known to have various physiological and functional consequences. Specifically, the β1-adrenergic receptors have a crucial role in the regulation of heart rate and ...

Introduction

The biuret test is a test that is used to test for proteins. More specifically, it tests for presence of peptide bond in a sample. According to Janiro, Sy, Yap, Llanos-Lazaro and Robles (78), the biuret test is one of the earliest methods that have been developed for determining protein content. The biuret reagent reacts with amino acids bonded but does not react with molecules like starches, sugars, vitamins and fats. A sample containing peptide bonds will react with the biuret reagent to form a purple colored complex. The amount of color produced is directly proportional to the amount of protein present ( ...

Abstract

The aim of this experiment was to determine the titration curves exhibited by two amino acids (Glutamic acid and Alanine) and use them to determine their Pka values and their isolectric point. Results from the experiment reveal several interesting facts as will be seen throughout this paper. As ampoteric compounds, this paper seeks to investigate these base-acid properties found in amino acids. Therefore, this paper will have the following basic parts; introduction, procedure for titration, discussion of titration results with reference to the titration curves as well as a conclusion. The introduction part will give an in-depth look at the ...

Question

Which of the samples of urine indicated kidney disease?
Experimental Design
This experiment aims at investigating the indications of kidney diseases using urine samples. This is by using Biuret reagents to identify proteins and Benedict’s solution to identify sugars in urine samples. Litmus papers were also used in the determination of the pH level of the urine samples.

Materials

The materials used in the experiment were:
- Safety goggles
- Eight test tubes
- Hot water bath
- Four urine samples labeled W, X, Y, and Z
- Tape and pen/pencil
...

Day 1 - Cell Culture: At First an LB/amp plate was labeled with initials, the date, the name of the bacterial strain to be used (BL21) and the plasmid (pDHFR). Then 10 uL of reconstituted BL21-pDHFR was pipetted onto an LB/amp plate using sterile tip.The bacteria was streaked into four quadrants of the plate and after this it was covered immediately to avoid contamination. The plate was placed upside down inside the incubator overnight at 37°C and once colonies have grown to ~1 mm in diameter, they may be stored in a sealed bag upside down at 4ºC ...

Introduction

The Extracellular signal-regulated kinases (ERK) pathway is one of the most important signal transduction systems in a cell. This pathway regulates a lot of the fundamental processes of the cell including cell growth control and also cell death. The ERK pathway’s basic structure includes a couple of phosphorylations. The pathway provides a channel for the passing of signals from receptor that is located on the cell surface to the DNA inside the cell nucleus.
The signal transduction process through the ERK pathway is a complex process. Initially, the signaling molecule, that is, the epidermal growth factor (EGF) usually binds itself to a receptor ...

Lab report discussion and results

Introduction
The experiment is aimed at studying about the protein content in the cells and tissues of various organisms and the ways of regulating the growth of some tissues in the body of the animals like in our case we used the rabbit tissues in determining the feasibility of the experiment. The process begins with the ability to look at the process of obtaining the protein from the sample given and how to quantify the amount of protein in such cells.

Method:

Protein extraction
The process of protein extraction involves three major processes which must be followed in ...

of HT22 Cells derived from Mouse Hippocampus

Sections I and II

Biology
Section I
1. Introduction
Fluorescent protein development research has gained increased knowledge about the range in the color palette available from the Aequorea Victoria jellyfish. The wavelength emissions in the blue to yellow range are now better understood. [1] Another area that has seen a great improvement in knowledge is the understanding of emissions of the monomeric fluorescent protein emissions in the orange to far-red spectral region. [1] The spectral region of fluorescent in the orange to far-red is derived appropriate reef coral species. [1] This area of research ...

Introduction

An enzyme is a protein with catalytic properties in biological systems. During an enzymatic reaction, a substance, called the substrate, binds to the active site of the enzyme and is then turned into one or more substance(s), called the product(s). The enzyme itself is not consumed or altered during the reaction. The enzyme activity depends on factors, including pH, temperature, buffer composition, enzyme concentration, substrate concentration, and the presence of inhibitors.
Enzymes have an optimal pH at which their activity is maximal. pH determines the ionization states of certain amino acids at the active site that are critical ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract
Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

- Purpose
Paper chromatography is a technique which is used to distinguish between the various components of a mixture. It helps to separate out the components of a solution and identify individual components. In Paper chromatography, filter paper is used as the primary material for conducting the experiment.
- Procedure
For the experiment, a beaker of 1L capacity is taken and filled with 100mL solvent. The beaker is known as chromatogram tank. Next, a filter paper around 12 inches long and 9 inches wide is taken. A light line which is 2cm apart from the bottom of the paper is drawn ...

Introduction

An eEnzyme is kind of a protein and, like all proteins, proton is composed of a chain (or chains) made up of the 20 different amino acids found in most living organisms. The sequence of the amino acid chain(s) determines the 3-D shape of the protein, and its shape to a great extent determines its properties. and it has a 3-D shape. Enzymes act in aqueous solutions to facilitate used in biochemical reactions by lowering the energy barrier, but they are not themselves changed or consumed by the reaction. This is referred to as catalysis, and enzymes are biological catalysts. to make ...

Epitopes constitute parts of antigens which bind themselves specifically on lymphocytes and receptors. As a result, they induce either antibody or cell mediated immunity. Basically, there are continuous and discontinuous epitopes (Yang & Yu, 2009, p. 77). The later are characterized by linear peptide fragments, usually of 19-12 mers. Usually, these are recognized by the distinctive T cells after they have been processed and presented on to MHC molecules. The later on the other hand comprise of 15-22 structurally complicated mers. They are characterized by a distinctive folding protein. This can be recognized by both the T and b helper cells while ...

This report describes a nutrition trial in which broiler chicks were divided into separate, randomly-positioned pens and reared under controlled conditions in which each of four batches was fed on a different diet treatment during the trial, which started on 18th August and ended 14th September. There was a control diet with no DDGS, two further treatments with 15% and 30% DDGS content respectively, and a fourth treatment also with 30% DDGS content but with added amino acid in the formula. The birds and feed consumed were monitored and weighed every third day, keeping records throughout. At the conclusion of the trial those ...

Introduction:

In 1976, Marion Bradford designed the Bradford method that is widely used in measuring the concentration of protein in a solution. When Coomassie Blue is added to a solution containing protein, the solution colour changes from brown to blue and this is attributed to the formation of a protein complex. The concentration levels of protein can therefore be measured by observing the dye color, which fully absorbed at a certain wavelength. The standard curve is used to calculate the concentration of protein because it represents the known BSA (bovine serum albumin) dilution. (Harvard, 2012)
A study by Daiet (2008) analysed murinae in ...

Malaria is a tropical disease that has claimed many lives over the numerous centuries gone by (reference). It usually caused by the genus Plasmodium. The vector for this parasite is a female Anopheles mosquito which transmits the pathogen through bite (reference). One of the major challenges in disease vectors is insecticide resistance. Vector-borne diseases are usually a burden in developing countries because it is not easy to reduce/control disease. In many parts of Africa, Anopheles gambiae has developed resistance towards pyrethroid insecticides which is used to control the malaria vector (Kumar, Dudley, 2007). It is feared that there are high chances of ...

LAB REPORT: ELISA PRACTICAL

Snakebites are clinically challenging because of the diversity of species and varying concentrations which impact the severity of envenoming on the victim. Other clinical problems can arise due to (a) unpredictable dose quality, (b) determination of appropriate dose, (c) local site of the snakebite and (d) route of injection (Warrell, 1996). The time that has elapsed from the snakebite event until presentation at hospital is the most important mortality-determining factor. The health status of the human victim is important for how local effects, systemic coagulopathy, and/or neurotoxicity exhibit (Warrell, 1996).

Intravenous doses are prepared from IgG of a single or multi- venom ...

Report for product transporting

Introduction
Preparation of protein EcCLpS in lab environment requires much time as well as controlled environment. ClpS protein is a member of the Clp family of proteins displaying modular structure. It is essentially a bacterial adaptor protein. Artificially prepared CLpS protein is sought by companies, especially pharmaceutical companies, for research; especially for ELISA, western blot experiments, antibody production, Right-Over –Expression Lysate and Left Control. It is researched for its application as antigen standard for colipase. As it is a specialized production, care has to be taken in its handling and dispersing to the end client. Specific conditions need to be maintained ...

INTRODUCTION

Proteins play an essential role in living cells. The basic forming units of those proteins are the amino acids which are made up of similar composition i.e. amine group, carboxyl group and a variable R-group. The laboratory tests to identify these amino acids work on identifying the variable R-groups that distinguishes each amino acid. It is very important to note that all amino acids with alpha-amino group turn purple ranging from pink to deep violet. Proline is the notable exception (only amino acids that turns bright yellow). Proline is the only one with its unique color. Other amino acids has ...

Prof:

Introduction
This is just one of the simple ways of identifying unknown compounds and separate mixtures. It is a separation technique that works based on the compound interactions as those compounds tend to move within a supporting medium. The compounds interact in two phases which are the mobile and stationary phase. The technique helps in analyzing, identifying, purifying and quantifying unknown separable mixtures. The mobile phase is either a liquid or gas which moves the solvent through the stationary phase during the process. The stationary phase is a liquid or solid component that is fixed in a place for the procedure.
Paper ...

List of Figures

Figure 1 Protein concentrations under the four conditions 7
Figure 2 Protein standard curve via Bradford assay at 595 nm 13
Figure 3 Protein standard curve via BCA assay at 490 nm 15

List of Tables

Introduction
Proteins are essential for life and we depend on them to interact with cellular molecules in order to live (Pace et al. 2004). Increasing the knowledge about protein solubility is significant for biological studies because increased protein concentrations enhance studies of cellular structures and functions (Golovanov, Hautbergue, Wilson, & Lian, 2004). Accurate protein quantization is also necessary for cellular studies (Johnson, 2012).
The environmental conditions of ...

Abstract

The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...

Abstract

Human activities have a significant influence on marine life. Pollutants from landfills, dumps, mines, farms, etc. can pollute the ocean and later through the food chain cause harm to all land animals and humans as well. Protein is a very important structural component of an organism. Using protein for energy is an inefficient form of metabolism and in the long run detrimental to the organism. A significant low level of protein content was noticed in Mytilus californianus or (mussels) obtained from the polluted environment when compared to the tissue of Mytilus californianus obtained from pristine environment. Organism that are ...

Abstract

This lab report discusses the Cellular Location of Metabolic Enzymes. Regarding methodology, the experiment was conducted in three sessions during the first session liver homogenate and cell functions were prepared. The first session was carried out by homogenizing the liver in an isotonic media. The Maltase and lactate dehydrogenase were determined in the second session, and protein concentrations were estimated in the third session. The Maltase dehydrogenase (MDH) and Lactase dehydrogenase (LDH) were used to catalyze the reactions in session two. The biuret reaction was used to estimate the protein in the last session. The relationship between absorbance at ...

1. Overview

The peptide hormones are proteinous in nature and may have 3 to 200 amino acids residues. This class of hormones includes all the hormones of the hypothalamus and pituitary, as well as insulin and glucagon of pancreas. Peptide hormones are named based on their endocrine activity rather than their chemical structure; for example, growth hormone is thus named because one of its effects is to stimulate growth. The structure of a particular peptide hormone can vary among different species, with some homology between close relatives. Some small peptides are identical; for example, GnRH is identical among mammals but different in ...

Chemistry

2. The nano carrier needs to remain in suspension in H2O, PBS, human serum, etc. What type of polymer do you need to use to decorate the surface of the nanocarrier to achieve this goal?
In order to maintain the nanocarrier in suspension in water, Phosphate-Buffered Saline (PBS), human serum a suitable solution a polymer is needed on its surface. The polymer used needs to accomplish three goals. The polymer needs to decorate the nanocarrier surface to enhance suspension and secondly, to prevent protein absorption. Another characteristic is that the polymer evades the immune system. PLGA poly(lactic-co-)glycolic ...

ABSTRACT:

A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...

Introduction

The SNAP-25 is the synaptosomal-associated protein 25 which generated different primary and secondary effects on animals and human being including death. The most important symptoms (Huang, 2004) of the SNAP-25 are:
■ Attention deficit hyperactivity disorder: This affects the attention, excessive activity and difficulties in control behavior. Extreme situations may cause death.
■ RS362584 polymorphism in genes causing neuroticism in living beings, including humans. The neuroticism causes anxiety, fear, worry and frustration in individuals. In extreme cases, its causes the death.

The SNAP-25 biologically interrupts and destroys links and the structure of the ribonucleic acid of the cells.

In a laboratory analysis, the degradation of SNAP-25 will cause death if it is degraded to less than 25%.
The ...

Wondering what should be the proper nutrition, it is necessary to understand what is embedded in this concept, to have an idea about the ways of cooking food, about the value of the constituent elements of products, about the regime and the amount of food that is required depending on the constitution, weight, age, sex, physical activities etc. If a person has developed and sticks to a proper habit in this area, and combines it with the systematic physical exercise, such person will probably have an excellent body metabolism.
After reviewing the analysis of my three days diet, I ...

Abstract

This paper is about the investigation of Parkinson’s disease, and in particular is involved with the examination of its symptoms, stages, causes, prevalence, diagnosis and treatment, and social impacts. Finally, the paper analyzes and evaluates two ongoing on Parkinson’s disease researches, which are also examined in respect to their impact on Parkinson’s disease patients.
Key words: Parkinson disease, Parkinson disease causes and impacts, Parkinson disease symptoms and prevalence, Parkinson disease diagnosis, and Parkinson disease ongoing research.

Idiopathic Parkinson disease (PD) is a progressively evolving disease with main manifestations resting tremor, muscle stiffness, slow movement and loss ...

Based on the initial symptoms, the patient most likely suffers from hypothyroidism or low thyroid hormone disorder. Further clinical tests and an examination of the family’s medical history reveals that the particular disorder could be Hashimoto’s disease; also called Hashimoto thyroiditis. Hashimoto thyroiditis is classified as an autoimmune disease. In autoimmune disease, one's immune system attacks the body’s tissue specifically the thyroid gland (Skugor & Wilder, 2006, p.2). By attacking the thyroid gland, the disease reduces the gland’s ability to produce thyroid hormones of substantial quantity. This condition leads to hypothyroidism. Thyroid hormone deficiency presents with ...

Introduction

The most widely used model when investigating the breast cancer is T47D cell lines. These cell lines are easily spread, easily manipulated genetically and when used in the experiment, it provides good results. They have been used to examine new therapies and the and the breast cancer pathobiology1.
Over many years HER2 has 25% - 30% of human breast cancers have been caused by gene amplification. The monoclonal HER2 and the EGFR are the two HER2-targeted therapies that are used for approval in the most clinics. During growth, HER2 is used to regulate the cell growth and assist ...

Introduction

The purpose of this experiment is to assist one to learn how different equipment in the laboratory is being use when carrying out the experiment. It tests the level of accuracy required when using different apparatus for measuring the volume. For very approximate result of volume, a beaker or conical flask is preferred when measuring volumes of liquids.

Accurate methods of measurement

This is done by the use of burette, conical flask and pipette. These measuring apparatus are discussed below.
When a much more accurate measurement is required, always measuring cylinders are preferred Measuring cylinders, which are much more accurate than beakers and conical.

Also volumetric pipettes and volumetric flask the mostly used apparatus used when measuring accurate volume.

...

Nutrient-based questions

1. Looking at your Nutrient Reports, which three (3) foods contributed most to your protein intake?
The food that contributed most to my protein intake was meat, poultry and eggs. The intake on the first, second and the third day were 18 ounces, 6 ounces and 8 ounces respectively. The total intake of proteins exceeded the target.
2. Looking at your Nutrient Reports, which three (3) foods contributed most to your total fat intake?
The food that contributed most to my fat intake was milk and yoghurt. I averaged 2 cups in the three days. Cheese ...

Psychology Summaries

Solomon, Caren G., Murray B. Stein, and Jitender Sareen. "Generalized Anxiety Disorder." The New England journal of medicine 373.21 (2015): 2059-68. ProQuest. 7 Apr. 2016 .Retrieved fromhttp://search.proquest.com.contentproxy.phoenix.edu/docview/1735349848?pq-origsite=summon
Generalized Anxiety Disorder (GAD) is a condition that creates excessive worry and concern in a debilitating manner for those afflicted with the condition. “According to representative epidemiologic surveys, the estimated prevalence of generalized anxiety disorder in the general population of the United States is 3.1% in the previous year and 5.7% over a patient’s lifetime; the prevalence is approximately twice as high among women as among men” (Solomon, Stein, and ...

Introduction

Avian influenza also is known as bird flu and results from viruses acclimatized to some birds. People may be infected with avian influenza when they get into contact with the infected birds or infected fluids from the birds (Capua and Dennis 10). In this report, the discussion is about the evolution of avian influenza, characteristics of the virus, epidemiology of the virus, as well as the mode of transmission. Moreover, the paper talks about the gene expression of the virus, infection of the human by the virus, evolution of avian influenza's vaccines, and criticisms surrounding evolution of the virus.

Overall features of the avian flu virus

...

A person’s nutritional status is largely determined by the diet he or she partakes in. Eating nutritious and healthy foods is essential for our bodies to obtain the nutrients needed. Moreover, the food we eat determines the caloric intake for the day, which is important when it comes to weight control. Furthermore, this paper is an analysis of my diet that was recorded by MyFitnessPal, an application that counts the calories of food one intakes and provides a summary on one’s macro- and micronutrients.
When my food intake from February 8 to 14 is analysed, it can ...

TA
ABSTRACT – Genetic engineering now has straight forward ecofriendly strategies to produce transformation in plasmids. The transformation process allows engineers to introduce DNa with specific desired capabilities into a host plasmid. In this way plants can be created that are able to resist a particular enemy or stressor without having to use toxics like herbicides and insecticides. The bacteria E. Coli was used to determine whether it can be made resistant to ampicillin or not. AmpR was integrated into the host cells and the tracer, GFP was used to identify expressed cells. The laboratory demonstrated that transformation by plasmids so ...

Section Number.

- Glycolysis refers to the process that takes place in the cytosol and through which a molecule of glucose is broken down into a compound called pyruvate ( Reece 167)
- Everthing is doubled since there is still more substrates required for respiration and therefore more energy is still produced as the substrate is not exhausted.( Reece 167)
- In the process of glycolysis 2 NADH, 2 ATP and 2 H+ are fomed. While in TCA 6NADH, 3 H+, 1 FADH2 and 2 ATP are formed. (Reece 176).
- The NADH is produced in the mitochondrion during the citric ...

Introduction:

“Dementia” defines a clinical syndrome exemplified by long-term decline in mental functional ability, such as waning intellectual abilities, in a conscious individual. The primary symptoms of dementia include loss of memory, decline in cognitive abilities such as learning and comprehension, judgment, communication, and loss of attention and orientation to oneself and time aspects. In addition, the patient may present hallucinations and delusions in form of paranoia in advanced stages (Raydin & Katzen, 2013).
The syndrome is a progressive condition that worsens with time. The experiences of the condition among patients may vary, just like the several causes that trigger the development of ...

Introduction

Biological reactions are those reactions that are involved in various activities, in the body including energy production. Reactions that produce energy are called exergonic reactions while those that use energy are called endergonic reactions. Enzymes enable chemical reactions to take place within a living system most of which are under a homeostatic constraint. In these reactions, enzymes function as a catalyst by lowering the activation energy necessary in a reaction (Cooper, 2000). Enzymes bring the reactants involved in a reaction closer together weakening the chemical bonds and this enable the reactions to occur faster compared to reactions that have no enzyme (Farabee, 2010).
...

Business Role-play: Negotiation

Business Role-play: Negotiation

Introduction

This negotiation took place between two different teams. The first team, made up of three team members, was tasked with selling a product to the second team for sale in retail stores in the Kingdom of Saudi Arabia. During this negotiation, both teams had a clear goal: to make a good business deal and build a good relationship with members of the other team. This was mainly a preliminary negotiation, which means that both teams were talking primarily about a single product. This product is a protein powder, which team 1—the sales team—claims is ...

Introduction

Huntington’s disease (HD) is an inheritable and neurodegenerative disease. It is characterized by progressive psychiatric, cognitive, and motor symptoms. It is significant to familiarize with the phenotypes since patients can present with one or more symptoms of the disease. Loss of balance and chorea are the first symptoms recognized by the patients. However, the families always notice personality or cognitive changes before first symptoms. The disease is most common in races from northern Europe. This paper is set to discuss the etiology and risk factors, pathophysiological processes, clinical manifestations, complications, as well as diagnosis of Huntington’s disease.

Etiology and Risk Factors

...

Rhabdomyolysis

Introduction
In normal conditions, muscles grow when certain activities cause the muscle fibers to become damaged and then subsequently regrown and developed through a series of cellular process. This activity occurs during the body is at rest. In other words, the continuous breakdown is normal for growing muscles as it allows them to become bigger through the introduction of myrofibrils, or muscle fibers.
However, there is a condition when the muscle fibers break down rapidly due to a number of reasons, and then they do not regrow at a corresponding rate. This condition is known in medical circles as ...

Keywords : Gene therapy, Sickle Cell Anemia, Stem cells, lentivirus, β-globin

Project type: GBE-492 Genetic Engineering Project
I. INTRODUCTION, BACKGROUND and AIM
Sickle cell anemia is a devastating genetic disease which causes premature death. Sickle cell disease (SCD) is caused due to single amino acid change (glutamine to valine at position 6) in β chain of hemoglobin molecule, which causes the hemoglobin molecules to aggregate. These aggregated hemoglobin molecules make the red blood cells (RBCs) stiff and deformed (sickle shaped). These deformed cells block the normal blood flow. Although this disease is well-characterized, but no long-term treatment 1 is still available for this disease.
The discovery of the sickle ...

Exercise 1: Determining the Caloric Content of Three Foods

Abstract:
In the beginning, I collected all the equipment that was required. After that, I emptied the balance and set it on zero and then, mass of the empty beaker was measured. Then, I added 50ml water into the empty beaker and checked the mass on the balance again. Afterwards, I take a foil paper and placed it at the bottom of the burner stand, and then I put the beaker containing the water on the stand for beaker. The initial temperature of the water was measured firstly, and then, I took a marshmallow and measured its weight with and ...

Aim

The experiment aims to study the effect of substrate concentration on the activity of polyphenol oxidase (PPO) from banana extracts using spectrophotometric techniques.

Introduction

An enzyme refers to a molecule that is protein in nature and works as a biological catalyst. The enzyme possesses three major characteristics. The first characteristic is that they act to increase the rate at which a given reaction takes place. This enables most cellular reactions to take place at up to million times faster compared to their rate in the absence of an enzyme. Secondly, most enzymes act specifically on a single reactant known as a substrate to produce the ...

Introduction

This analysis will focus on the excess intake of saturated fats and cardiovascular disease. The analysis is based on the report detailing my nutrient intake recorded for two days starting from September 4, 2014 to September 6, 2014. According to the analysis, my diet during the two-day period predisposes me to cardiovascular disease since it is high in saturated fats. According to the food guidelines given by USDA, saturated fats should contribute less than 10 percent of the total daily calories. However, my nutrient report indicates that saturated fats contribute 12 percent of my total daily calorie intake. Consequently, ...

Objective 2

Introduction 2
General background 3
Resistance mutants 4
Hemoglobin mutants 4
Sickle Cell Anemia and Genetics: Background Information 5
Connection of Sickle cell with Malaria 6
Method: 7
Introduction of Recombinant DNA technology in diagnosing disease: 8
Discussion (implementation of recombinant DNA technology to solve the issue) 9
Conclusions 10

References 11

Objective
This report targets to study a group of people that has developed resistance to malaria. It will focus on the use of recombinant DNA technology to study this cohort and gain insights into the nature of this observation. This ...

Introduction

Monitoring one’s diet is important since it informs dietary choices that one should make in order to achieve and maintain healthy lifestyle and good health. Therefore, I took part in recording my food intake and exercise habits for three weeks. Nutrition determines many aspects of human beings. As the common adage goes, ‘we are what we eat.’ This adage implies that what we eat defines many of our aspects. Nutrition is indispensable to human health. Similarly, physical exercise plays a major role in promoting good health. For instance, it improves general health and helps achieve glycemic control among people ...

Nutritional Report

- Introduction:
This report analyses my nutritional intake for three days. I have created a record of my food intake amounts for three consecutive days. The goal of this exercise was to record how balanced my diet is, whether I am within my recommended calorie and nutrition intake and what are the factors that affect my food choices. It is also an initiative at improving the quality of my diet. It is an attempt at finding healthier food alternatives. The motive behind this is not to participate in futile calorie counting, but to make sure I am giving my ...

Microbiology laboratory report

Abstract
Bacteriophages are intracellular parasitic viruses that infect bacteria to carry out their multiplication. During the lytic infection cycles, phages lyse the host cells forming a clear zone called plaque on the agar media. The experiment is aimed to measure the number of plaques created when the virus T4 infects the host bacteria and their genetic material integrates into the genetic material of the host cell. This happens because the E.coli becomes resistant to T4 bacteriophage. The T4 phage was diluted in a test tube and then E.coli bacteria was added in the tubes. The mixture was poured with soft ...

Introduction

Pain is an essential part of the body’s defense system which is perceived as an unpleasant feeling. Pain produces a quick warning to the central nervous system to start motor response as a minimization of harm. Lack of pain perception is dangerous can cause grave problems such as Auto-amputation, corneal scarring or self-mutilation. Researchers in the medical profession have distinguished between pain and what is known as nociception. Nociception occurs when signals get to the central nervous system as a result of the activation of nociceptors (Svokos & Goldstein, 2009). Nociceptors are special sensory receptors which relay information regarding tissue damage. Pain ...