Essays on Safranin

Identification of mystery microbe based on its morphology and biochemical properties

The experiment was conducted to detect the species of a given mystery microbe. The microbe was subjected to a battery of tests in order to identify its physical and biochemical characteristics. Based on the tests, different possible microbe species were eliminated to finally arrive at the right conclusion. The possible microbes included Escherichia coli, Providencia alcalifaciens, Enterobacter aerogenes, Staphylococcus epidermidis, Klebsiella pneumonia, Micrococcus luteus, Serratia marcescens, Mycobacterium smegmatis, Sarcina aurantiaca, Pseudomonas aeruginosa and Bacillus licheniformis. The first test conducted was to detect the Gram staining properties of the given microbe. This helped in identifying whether the given microbe had a thin ...

UNKNOWN #16

SUMMER 2013 INTRODUCTION Purpose The purpose of the lab is to separate characterize and identify microbes from unknown mixed culture using the morphological and biochemical methods.

Theory

The process of identifying bacteria is a systematic and careful process which uses various techniques to narrow down the bacteria types which are present in an unknown culture of bacteria. This process produces advantages for many factors of research of microorganisms and assists in the proper treatment of patients (Aneja 10). Several tests were performed to give the fermentation abilities, availability of specific enzymes and specific biochemical reactions.

MATERIALS AND METHODS

The experiment started ...

Part 1

1. 1. Eye piece 5.Arm 2. Objective lens 6.Coarse adjustment knob 3. Stage 7.fine adjustment 4. Base 2. What is the procedure (step by step) which you followed to do gram staining? (20 points) 1. Obtain a tissue sample for the gram stain. 2. Two or three drops of the sample were placed on the glass slide. 3. Use the electric slide warmer, heat the smear. Alternatively, this can be done by quickly passing it over a flame. Although should be very quick to avoid distortion. 4. Add crystal violet to the ...

Component One

The Data Quality Objectives (DQO Process) is a planning process that has been developed by the United States Environmental Protection Agency. This particular planning process is used when environmental information or data is used to make a choice between two specific alternatives or to make a derivation of contamination estimate. The process is also used in the development of acceptance and performance criteria that find use in the clarification of study objectives, defining of appropriate data type and the specification of potential errors of decisions that are then used as the basis for the establishment of the quantity and the quality of ...