Questions and Answers
For each of the following sequences, fill in either the DNA (template strand), the mRNA sequence, the tRNA anticodons, or the amino acid sequences that have been left blank. If several sequences might work, choose any one. Start translation with the first codon for each line (don’t worry about an AUG)
DNA T A C T G A T C G A C C C C C A T A A T G A A A A T C
tRNA U A C U G A U C G A C C C C C A U A A U G A A A A U C
2. DNA T A C C G C T C C G C C G T C G A C A A T A C C A C T
tRNA UAC CGC UCC GCC GUC GAC AUU ACC ACU AA MET ALA ARG ARG GLN LEU LEU TRP STOP
DNA TAC CAC CCC CGU AUG GCU GGG AAU AGC
tRNA U A C C A C C C C C G U A U G G C U G G G A A U A U C
AA MET VAL GLY ALA TYR ARG PRO LEU STOP
DNA TAC TCT CCA AAA AAA TAC CAA CCA ACT
tRNA UAC UCU CCA AAA AAA UAC CAA CCA ACU
AA MET ARG GLY PHE PHE MET VAL GLY (STOP)
5. DNA T A C CTC ACA CTA CGA A T G TTG GGA ATT
tRNA UAC C U C ACA CUA CGA AUG UUG GGA A U U
AA MET GLU CYS ASP ALA TYR ASN PRO STOP
Fill in the DNA sequence (A,G,C,T). Be sure to label the 5’ and 3’ ends of each DNA strand. Circle a nucleotide. Put a box around a base pair. Draw an arrow to a set of hydrogen bonds. Label a phosphodiester bond.
5’ end 3’end phoshodiester bond
On the diagram below label or draw in:leading strand, lagging strand, template strands, replication fork helicase, topoisomerase, DNA pol, RNA primers, primase, ligase, Okazaki fragments, and 5’ and 3’ ends of ALL DNA molecules
Template strands
Replication fork helicase
primase
leading
topoisimerase strand
template strand okazaki
fragments
DNA pol RNA
primer
ligase
3’ end
5’ end
Lagging strand
Draw a replication bubble and use the diagram to explain how DNA replication is
Semiconservative –
Semi-discontinuous
Antiparallel
Bidirectional
Template strand
5’ 3’
Leading strand
3’
Lagging strand 3’
3’
Template strand
