Essays on Sequences

Introduction

Exom sequencing is also known as whole exom sequencing (WES). This sequencing technique is used to sequence all the expressed genes in a genome also called as exom. It is one of the most widely used targeted sequencing methods. The exom which is the protein coding region of the human genome consists of less than 2% of the genetic code. This region is responsible for containing more than 85% of known disease-related variants. Thus, whole exom sequencing is a cost-effective alternative to whole genome sequencing (WGS). This technique is efficient in identifying coding variants across a wide range of ...

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Introduction

Numerous types of color processes in film has been witnessed throughout time. It is evident that some color has been more successful in film than others. As a matter of fact, some colors have remained dominant for an extended period while others fade away quickly if not required. In the future, the film industry will go ahead to experience advanced technology that will enable filmmakers to use more attractive and sophisticated colors. Probably an essential point to remember is that the evolution in film has been undergoing a gradual transformation since time immemorial. Like a century ago, people thought ...

Dream sequences have appeared as an element in various media since at least the time of the Ancient Greeks, with Aeschylus seemingly using the device in his play The Persians, but it was Hollywood which really brought the idea of the dream sequence into common knowledge. These sequences, in both film and books, are used for a variety of reasons: they can be used as a means of gaining insight into a character’s mental state, or some event of their past which has bearing on the present happenings. Alternatively, dream sequences may be used as a means of ...

The development of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has transformed genetics and gene editing. CRISPR’s precision and efficiency have allowed scientists to control and modify genetic material with unparalleled precision. This technology, however, presents a slew of ethical issues about its possible ramifications and implications. This essay will outline the CRISPR technology, discuss the ethical problems with CRISPR and conflicts surrounding it, as well as argue whether gene editing is ethical in general.

What Is the CRISPR Technology?

CRISPR technology is a genetic technique that allows researchers to alter particular genes in an organism’s DNA. The method employs a class of enzymes ...

Psychology: Baddeley’s Working Memory

Introduction – Baddeley’s Working Memory Short term memory is an important aspect in human lives as it influences behavior and outcomes in daily lives in more ways than one – of which the role played in development of working memory is an important factor. Even though short term memory is considered to have significant differences from working memory, they are considered to be related by eminent psychologists. The most common factor behind this perspective is that both short term memory and working memory are involved in the storage of information temporarily in an individual. Working memory can be best described ...

A proposal in partial fulfillment of the requirements for the degree of

Introduction Wheat (Triticum sp.), an extensively adapted crop, is one of the primitive and primary staple food crops in the world. Wheat is cultivated from temperate; dry to irrigated or rain-fed areas, to warm, humid or cold conditions. Such a high adaptability is attributed to its intricate genome (Acevedo et al., 2002). Wheat cultivation covers more land area compared to other commercial crops and endures to be an essential food grain for humans (Curtis, 2002). Estimations reveal that by 2050, the population of the world will reach 9.1 billion (34 percent greater than today); correspondingly, the food requirement and ...

Transcriptional regulation of gene expression

Gene expression in eukaryotic cells is regulated at transcriptional, post transcriptional and translational levels (Tomizawa,  Lekstrom-Himes, and Xanthopoulos 17). Transcriptional control is the primary means of regulating gene expression. Eukaryotic transcriptional control functions at three levels: modulation of the activities of activators and repressors; variations in chromatin structure; and effect of activators and repressors on assembly of initiation complexes (Lodish et al. 368). Regulation of transcription initiation is the most widespread form of gene control in eukaryotes. However, in some cases, transcription may be attenuated and regulated at subsequent steps, such as during or after the transcription process (Cooper ...

Questions and Answers

For each of the following sequences, fill in either the DNA (template strand), the mRNA sequence, the tRNA anticodons, or the amino acid sequences that have been left blank. If several sequences might work, choose any one. Start translation with the first codon for each line (don’t worry about an AUG) DNA T A C T G A T C G A C C C C C A T A A T G A A A A T C tRNA U A C U G A U C G A C C C C C A U A ...

The article "Scientists Talk Privately About Creating A Synthetic Human Genome", written by Andrew Pollack, is about the research made by the author and other scientists in the sphere of the coordinated oscillations. They have managed to contemplate the human genome fabrication within the modern synthetic biology. They took various forms and components, linked them in order to create differently new forms with the aim of creating the new human DNA by use of chemicals. In general, synthetic biology considers the switches of genes, and the scientists made there investigations within that sphere. In recent years, instead of the ...

Ang Lee is director of wide range of films, and each of them provokes controversy and interest among the audience and critiques. In every taken film the sequences are combined in a unique narrative with its questions and much less answers. Lee is a representative of so called independent genre, meanwhile it should be noted that his films could hardly be titled by any kind of clichés (Siraganian 1). Nevertheless, having in mind the alternative, not common point of view and the craft of directing, all his films are definitely independent. The core essence of Ang’s style ...

There are different periods in the history of countries that play more or less significance roles. No doubt that First and Second World Wars immensely influenced the life and political situations almost in every country. These were the most disastrous event and their aftermaths were often disastrous too. In spite of these tremendous periods in the world’s history there were no less and maybe, according to some views, more important period in the history of America in the twentieth century. The period of the 60s is treated now as the time of innovations in culture, science, music and ...

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Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

My observation was based on three documents. Both of them considered a particular set of language observation that had relations with English as a second language. These observations are important for Language studying. I will provide my analysis and observation below.

1st Journal Article

Vocabulary is currently identified as a fundamental part of language development and might be among the most significant components for language learners. Yang et al. (315), makes an observation based on an e-learning system that is known as MyEVA about a vocabulary strategy which provides learners who study English as a second language in making improvements on their ...

1. a. The pKa value of an amino acid is an indication of how strong or weak an amino acid is, based on the said acid’s dissociation constant. An amino acid that has a high pKa value is weaker than on that has a low pka value. Given that an acid’s dissociation constant is Ka, the pKa value is given by the formula: pKa = -log10Ka pH = pKa + log ([A-]/[HA]),It follows that for Cys12, 7.4= 7.2 + log([Cys-]/[HCys]) 7.4-7.2 = log([Cys-]/[HCys]) 0.2 = log([Cys-]/[HCys]) 100.2= Cys-/HCys Therefore, Cys-/HCys = 10-4Similarly, ...

Hayao Miyazaki does not like to give interviews and as a great cinema master he is permitted to avoid them. However, judging by those interviews available, it is possible to conclude that he appears to be rather pessimistic in respect to the future, in general. This is rather interesting, since his cinematic works do not have any element of pessimism. Instead, the majority of his movies provide viewers with the glimpse of hope. For instance in Spirited Away, Chihiro manages to survive the hardship without any complaints and even proposes a fantastic ability to deal with them effectively. In ...

Question 1: Types of Project Life cycles

A project is well stated when the scope, time and cost are clearly defined. In other words, for an activity to qualify as a project it must have a definite scope, timeline and the cost likely to be incurred. The project life cycle denotes the various stages of the project. Projects must go through four main phases, initiation, planning, execution and termination (Rowley, 2013). This is what is called the project life lifecycle. Various types of project life cycle exist. The most common ones are predictive, iterative, incremental and adaptive life cycles. The differences in the types of project ...

In simple terms, Gattaca, a 1997 movie, can be described as a sci-fi adventure that is combined with a crime narrative. The viewer is treated to a relentless action throughout the movie as the police engage in a search for a murder. As the story unfolds, the viewer’s attention is turned to a young lad known as Vincent, also named ‘Jerome’ by his workmates. Vincent is trying to overcome his biological challenges that he was born with in his quest to become an elite astronaut in a space program run by Gattaca Corporation. As the two stories unfold ...

Deadpool

Introduction The film score which is also called the background music of the film also forms the film’s soundtrack which is timed to begin and end specific points in the movie. It is also used to heighten the dramatic plot and the emotional impact of the film for the audiences. It is written under the guidance of the director because it is important that the music used in the film would be related to the scenes or sequences. The film industry acknowledges the importance of the background music and soundtrack of the film because it is used to ...

Baghban

Introduction Bollywood which is the Indian film industry is the second largest film industry in the world next to Hollywood. The industry has already gained undeniable success not only in India but worldwide. One of the distinctive features of a Bollywood film is the presence of songs and dances in the sequences of the movie. This is the main reason why Bollywood films are gaining popularity in the international film industry. The songs and dances have been an important part in a movie and it started from the early days of Bollywood. Every film must contain at least four ...

Introduction

Trillions of microbes constitute the “Metagenome” of the human gut. This is the organ called microbiome, which is highly essential, and offers the host benefits such as metabolic abilities, pathogen protection, immune system enhancement, and GI tract developmental modulations (De Fillipo, et al.). In their study, three questions regarding the evolution of the human microbiota have been raised. They are: (1) how does the partitioning of bacterial diversity between and within two populations studied? (2) Does there exist a correlation between the diet and bacterial diversity?; and (3) considering the varied geography and hygienic conditions, what is the bacterial ...

Introduction

The treatment for multitudes of diseases and disorders resulting from genetic reasons have been ambiguous since long as the only cure could be the correction of genes and DNA in the specific region. Gene therapy gave a promising horizon to the correction of such genetic defects whereby molecular tools are applied to reverse the mutation or any other changes in the DNA. The advancing biomedical research have offered several tools for gene therapy since last few decades that have been able to perform the desired roles but with one or the other limitations. Lately, CRISPR-Cas9 based tools for gene ...

The payoff for the planning effort comes in the implementation of the plan. The planning process creates a road map that the marketer must use to get where e want to go. Marketing planners have an advantage in that the hand –off from planning to implementation is likely to be something other than it is for other types of planning. Indeed, the same parties are likely to be involved in both activities. In order to approach plan implementation systematically, it is important to include adequate detail in the rejected plan and develop and implementation matrix. The project plan systematically ...

Questions and Essays

Question #2 It might be argued that the character of Ned Kelly is a Robin Hood-like character. A 19th century outlaw, Carey illustrates him as the conventional bad guy with positive qualities. This is why it can be said that Kelly is a Robin Hood like character, as Robin Hood executed certain aspects that were not necessarily morally correct, or ethically right - but nevertheless allowed for positive outcomes. Much of the novel consists of Ned encountering bad luck and being manipulated and lied to. As such, he is forced into the seemingly bad guy lifestyle ending up in ...

Abstract

Transformation remains, and hugely significant tool in extending the borders of life sciences and medicine. It can be referred as fixing intracellular DNA into a bacteria or organism through the vector, thus transforming it. This paper uses plasmid pUC18, self-reproducing circular/spherical strands of genes that may be modified. After the bacteria absorb the vector, some proteins not usually synthesized will occur. In this study, a system has been developed to monitor the transmittance of different DNA (genes) from a genetically transformed strain of Saccharomyces cerevisiae to E. coli. This mechanism is centered on yeast strains that contain several integrated ...

Beyond doubt, D.W. Griffith is one of the stalwarts in the domain of cinema who has left an immortal mark in the minds of the film critics and the audience with his seminal works of art that have been deemed to be among the best the medium of art has ever seen. The primary importance of Griffith’s cinematic works lies in the use of innovative filmic techniques that went on to define the language of cinema in the future years. A close scrutiny of the films like The Birth of a Nation (1915) and Intolerance (1916) would enable ...

Article Review on Polytene Chromosomes and Structural Abnormalities

Background The interphase stage during cell division condenses the genetic material i.e. DNA into chromosomes and in salivary glands of Drosophila melanogaster, polytene chromosomes appear characteristically that are a result of repeated replication cycles but no disjunction. They have dark bands alternating with light interbands. The hypothesis tested in the research work performed by Zhimulev et al in 2014 was that the interbands incorporates transcripton initiation sites i.e. promoter binding areas that extend to coding areas in band regions. Using the modENCODE protein profiling data, DamID genome mapping has earlier classified various chromatin states. The current study performed cytological ...

While most people are familiar with a number of leading theorists in psychoanalysis, particularly Sigmund Freud, and possibly Adler and Jung, most people would not be familiar with Karen Horney, considered to be the “fist psychoanalytic feminist” (Hergenhahn & Henley 525). Horney disagreed with several concepts introduced by Freud, especially his focus on unconscious sexual motivation and the division of the self into 3 parts: id, ego, and superego. She believed that social experiences had a greater influence on the creation of self. During the Depression, she found that her clients’ problems were caused by unemployment and the inability to ...

Abstract

This paper analyzes the importance of generator synchronization to the power grid, the required conditions to perform this procedure and the consequences of imprecise synchronization. For a generator to be synchronized to a grid, the voltage magnitude, frequency, phase sequence and phase angle must match. Manual synchronization depends on an operator to modify generator voltage and frequency, confirm phase sequences of generator and grid, and monitor phase angle difference to timely initiate breaker closure. Automatic synchronizers perform all these function through a computerized system that consists of complex algorithms and control systems, and are categorized in phase lock type ...

Lachman, M. E. (2004). Development in midlife. Annu. Rev. Psychol., 55, 305-331. In this article, based on the analysis of contemporary foreign and domestic philosophical, sociological and psychological literature have been considered theoretical interpretation and empirically recorded phenomenology "midlife crisis / mid-life." Author used the method of biographical interviews and have shown the common strategy for coping with the crisis, the dependence of its manifestations on the type of self-identity and gender of the respondent. The author rightly suggested that the crisis could be caused by the coincidence in time of several adverse events related to deviant factors: divorce, ...

Health abnormlities of several kind require a thorough assessment in terms of management. With the technical advancements, medical field had evolved to simplify the complex assesssment tasks. For instance, medical imaging has come up to make feasible the analyis of body parts through the production of images. There is a need to know much about this particualr area. In such context, the present description deals with a topic Magentic Resonance Angiography (MRA) of the remal artery with a special emphasis on the 3D gradient echo sequencing in MRI. Briefly, magnetic resoannce angiography constitutes a kind of MRI (magnetic resonance ...

IT-experts treat data mining (storage) and retrieval as the most important application of the data warehouse technology (see, e.g., Inmon, 1992; Inmon, 1997). In particular (or most largely), this refer to the retrieval of the latent predicting information from large databases (Edelstein, 1997; Thearling, 1998). In the present essay, most important and promising trends of automatic tools to store, mine, retrieve, and process data are reviewed.

Integration of text, data, code, and flows

In DBMS (database management systems) area, the main attention was always drawn to the organization, storage, analysis, and retrieval of structured data. The development of Internet has shown that more complex data types ( ...

It is considered that Hayao Miyazaki does not like to give interviews and therefore avoids them. However, judging by those interviews available, it is possible to conclude that he appears to be rather pessimistic in respect to the future in general. This is rather interesting, since his cinematic works do not have any element of pessimism. Instead, the majority of his movies provide viewers with the glimpse of hope. For instance in Spirited Away, Chihiro manages to survive the hardship without any complaints and the ability to deal with them adequately. In case with Howl’s Moving Castle, the ...

Inductive reasoning depends on past encounters to frame a conclusion. Along these lines, gaining from past encounters one can utilize inductive thinking to arrive at a conclusion. In the expert life where this can be utilized is at the enlistment space. From past encounters on which kind of or aptitudes required for an occupation, we can finish up what kind of people will be fit for the employment. If there should arise an occurrence of deductive reasoning, we can utilize sensible explanations to go to an induction. For instance, in day by day life we can utilize this aptitude ...

Patterns of development can be referred to as the emotional, psychological and biological changes that happen in human beings from birth and until the end of adolescence stage (Shaw et al. 677). It is a perpetual process with foreseeable sequences though it is valuable for every child. Typically patterns of development do not advance at an even rate, and every stage is impacted by the previous type of development. Development in human beings occurs due to genetic factors leading to maturation. Also, learning and environmental factors play a role in the development of children. Typically, the first five years ...

Many critics called the film Sholay (1975) directed by Ramesh Sippy the best film in the history of Bollywood, or the "Film of the Millennium". The critics praise it so much so that several critics insist that "innovation in Hindi films ends with Sholay and that everything after that is repetitious trash" (Banker 55). This picture is a mix of crime thriller with the elements of "Japanese samurai epics and American and Italian Westerns, most obviously The Magnificent Seven (which was based on Akira Kurosawa’s Seven Samurai)" (Banker 55). The director involved a number of best Indian actors ...

LIST YOUR INSTRUCTOR’S NAME

LIST THE COURSE LIST THE DATE THE ESSAY IS FILED Berlin, Symphony of a Great City (1927)

Introduction

A brief glimpse into Berlin, Germany, and film and documentary production in the 1920s will provide historical context to better understand and appreciate the quality and value of this innovative documentary. Cultural life blossomed after the 15 million deaths and destruction of World War I. In the 1920s, the German film industry, despite uncertainties and financial difficulties, became firmly established and very productive (Goesch 61, 72). The emphasis on Expressionism became reality, objectivity and good times in the late 1920s. The art ...

The monitoring of the Gigabit Passive Optical Networks (GPONs) is based on the optical coding (OC) technique and which is the most cost-effective monitoring technique. The performance of the OC technique relies on the design of the implemented hardware and on the design of the address code chosen. With an increasing number of GPON users, the address code selected must has the ability to support a number of code-words that are equal or larger than the available users. The main purpose for this research is to improve the GPON monitoring system using the OC technique. This research has developed ...