Essays on Protein

VAMP7 Modulates Ciliary Biogenesis in Kidney Cells

Epithelial cells elaborate specialized domains with distinguishable compositions of lipid as well as protein, which include the basolateral and apical surfaces together with primary cilia. For proper functioning of the cell, maintenance of these domains; identity is necessary and requires the selective and efficient SNARE-mediated vesicles fusion that contain newly produced and recycling proteins that have the suitable target membrane. There exist many pathways to carry freshly synthesized proteins to the kidney cells’ apical surface, and the post-Golgi SNAREs, or VAMPs that participate in these distinguishable pathways have not been discovered. There has been implication of VAMP7 in delivery ...

Introduction

Nutritional needs vary according to the stages of life. From infantry to adultery nutrition is a vitally important aspect of such characteristics as mental development, growth, sexual development and resistance to illnesses. That is why maintaining a well-organized healthy diet is crucial for providing the population with appropriate development and omitting health problems.

Infants

At the infantry stage the differences in nutrition for boys and girls are generally not observed. Infants are first of all fed through breast milk, which is preferable in all cases, and infant formula, if breast feeding is not available. Infants receive most calories from fats, namely omega-3 ...

Q. 1. Explain how gel filtration chromatography (size exclusion chromatography) separates molecules on the basis of their mass/size.

A. In gel filtration, porous beads are packed into a column. The pores are of different size. The column is then packed with a buffer solution. This buffer solution is present inside the beads as well as around it. When molecules of different size pass through the columns, the smaller molecules pass through the pores and take a longer path. The larger molecules, however, travel around the beads. Thus, larger molecules travel faster than the ...

Lysine

Amino acids are organic molecules, the monomers of proteins that contain both amino and carboxyl groups. Proteins of a cell mostly contain 20 types of amino acids that differ in the side chain known as R group. Therefore, the properties of the amino acid in protein fully depend on the characteristics of the side chains.

Lysine is an essential amino acid, that means it cannot be synthetized in the human organism. The side chain of the lysine molecule contains an additional amino group attached to the carbon skeleton. Because of the positive charge in the side chain, the charge ...

The process of DNA replication at biochemical level

DNA or deoxyribonucleic acid is a biopolymer that encodes all the structures needed for the organism’s lifespan and development. In a cell it is located in the nucleus, mitochondria and chloroplasts. DNA consists of four types of deoxyribonucleotides, the sequence of which is used to store the data.

The essential characteristic of DNA molecule that allows it to pass the stored information to the next generations of cells or organisms is the way of self-reproduction known as replication. The molecule consists of two complementary chains that are held together with the help of hydrogen bonds between the complementary bases. ...

DNA (Deoxyribonucleic Acid)

Deoxyribonucleic acid, DNA, is the primary structure that carries the genetic material in cells of living organisms and some viruses. The DNA is made up of strands of nucleotides that are made up of nitrogenous bases, a phosphate compound and, a ribose sugar. The nucleotides form a ring or ladder that over lapses to form double helix strands of polynucleotides. DNA aggregates to form the chromosomes that are strictly found within the nucleus of the cell. Some DNA are found in the ribosomes outside the nucleus. In the DNA structure, the series of the bases is arranged, and the sequence in one ...

Gene expression refers to the mechanism by which information from a gene directs the synthesis of proteins. This process is utilized by eukaryotes and prokaryotes. Gene expression involves various steps. These includes; transcription and translation. The central dogma indicates the flow of genetic information from the Deoxyribonucleic Acid to Ribonucleic Acid and synthesis of the required protein.

Transcription is the production Ribonucleic Acid copies of Deoxyribonucleic acids in the nucleus. The DNA consists of two anti parallel complementary strands, each having 5’ and 3’ ends. The strands serve as a template strand for the production of an RNA transcript. The ...

Causes of ALS

method to determine the Causes of ALS Blood samples of 20 individuals will be collected to determine the cause of Amyotrophic Lateral Sclerosis (ALS). A consent form will be signed. In our study, mutations will be checked. DNA of the selected individuals, who may have higher chances of developing ALS, will be checked and genome scan will be analyzed with proper applications such as Genehunter v2.1. Polymerase-chain reactions (PCR) will be performed on DNA, and PCR products will be sequenced in the proper facilities.

Results of Causes of ALS

ALS can be hereditary and 5% to 10% of all cases of ALS are ...

Introduction

Proteins are food substances that are consumed with intention of maintaining the human hygiene and improving the status of health of living beings. However, there is a differentiated class of proteins that is referred to as the deceptive proteins. Grammatically, the term deceptive means something that is likely to create a notion that is different from the truth. In this case, the term deceptive protein is used to mean a category of protein that is intended to ensure balanced diet but instead creates a different impact in the performance of the body organs. For instance, deceptive proteins are said to create ...

Introduction

Capsular polysaccharides are of high molecular mass and low molecular weight cell-surface polysaccharides of Gram-positive and Gram-negative bacteria (Wang et al. 2000). They facilitate evading the immune system; therefore, the production and extraction of this layer is a prospective therapeutic target (rcsb.org). In the Gram-positive bacterium Streptococcus pneumoniae, strain TIGR4 the Cps4B is tyrosine protein phosphatase CpsB. The phosphatases have capability to dephosphorylate their analogous autokinases, instead of their similar functionality equivalence; they don’t demonstrate sequence homology. This capsule or CPS act as an interface border between a microbe and its environment that presents an expansive biological and clinical significance. ...

- Factors in phenotypic variations Factors in phenotypic variation consist of: Variation in phenotype (Vp); Variation in phenotypes caused by genetic variation (Vg); Variation of phenotype caused by environment (Ve); Variation in phenotype caused by interaction of genotype and environment (Vgxe). In addition to the above factors, phenotypic variation is also influenced by effects of genotype on phenotypes.

- Additive genetic variation formula shows that Va is influenced by phenotypes (p), additive (a) and dominance (d). As population evolves, the values of p, a, and d change. Consequently, Va value also changes. Based on the narrow sense heritability formula, change in Va ...

Formation of bone during foetal development occurs through the process of ossification. This process is extremely important to proper bone length, density, and uniform skeletal formation. It is driven by a complex network of genes that code for signalling proteins whose function is to turn the ossification process on, off, or slow it down. Defects in these genes can cause problems with the ossification process, and can lead to conditions of skeletal dysplasia (improper formation of bone). One such condition is Achondroplasia, one of the most common forms of dwarfism, or congenital short stature (Lister Hill National Centre for Biomedical ...

There are a variety of diets that all claim to do the same thing: help people to lose weight with minimal effort, in a short amount of time. The Dukan diet, which falls under the high protein diet category, has been referred to as the “Princess Diet,” was designed by a French doctor by the name of Pierre Dukan and introduced to the United States in 2011. According to the claims of the diet, it will help those who follow the guidelines to lose up to 10 pounds within the first week while eating as much of the approved foods ...

The amino acid is a hydrophobic and aromatic essential amino acid. The compound is relatively non-reactive because of the benzene ring in its structure. Since it is nonpolar, the amino acid does not dissolve in water. Due to its hydrophobic nature, the amino acid is buried in the hydrophobic core of protein structures. However, the aromatic side chain in the structure of the amino acid affects protein structure by staking with the aromatic side chains of other amino acids. The side chain of the amino acid is relatively non-reactive and as such is not often involved in the functions of ...

Deoxyribonucleic acid, DNA, is involved in the manufacturing process of all proteins in the body. It synthesizes proteins that repair and replace worn out tissues in the body. Stem cells (undifferentiated cells with the ability to differentiate) researches have shown that cells from some regions of the body can differentiate into different tissues. Therefore, they can replace worn out tissues and hence function as a repair mechanism. These cells can be induced to become tissue or organ cells. Scientists argue that perhaps the stem cells may carry out similar functions as DNA hence can replace it. This paper seeks to ...

The daily requirement for protein is equivalent to 0.8g/kg body weight. This implies that the total protein intake should never exceed this rate. The calculation can also be based on the total calorie requirements per day. In such a case, protein should account for an average of 25% of total calories in a day. The range is between 10-30%.

This requirement is the average intake for an average woman with an averagely active lifestyle. However, this can increase when the activity level increases. Furthermore, the requirement also increases during pregnancy or when recovering from an illness. Under such circumstances, the intake ...

Protein degradation is a regulatory process of all living beings that helps to weed out proteins that are faulty in nature. The fault maybe in their sequence or folding. The proteins are degraded via ubiquitin-proteasome system (UPS). The proteasome is made up of an outer activating module and an inner core particle (CP), which in turn is made up of outer α subunits and inner β subunits. The α subunits guard a N-terminal gates pore, which open up when proteasome activators (PA) binds to it. The β subunits have the active sites that can broadly accommodate different faulty proteins for degradation. ...

The experiment aimed to show that Blm10 protein elevates the turnover of proteasomal substrate through an active gating mechanism. This was to be done by showing that Blm10 enhances degradation that is proteasome-mediated in vitro using hypophosphorylated unstructured tau-441 as the substrate.

The objective was achieved using various stains such as BY4741, yMS63, yMS94, yMS122b, yMS131, yMS152, yMS159, and yMS476 among others. To start with, the proteasomal activity was assayed by determining gate opening in yeast CP/20S proteasome by the C terminal peptides. Phenotypic analysis was also done by growing the stains overnight in YPD and diluted to a density ...

Part I

The Zone diet is a diet that advocates the consumption of calories from protein, fat and carbohydrates using a balanced ratio. In this way, the diet aids one in increasing wellness and loosing fat by decreasing cellular inflammation. This is how the diet is based on the principle of controlling hormones to maintain good healthy conditions within the whole body system. It also controls gene expression to ensure a better and longer life.

Hormonal balance is essential since it affects and influences all the components of wellness: blood chemistry, energy utilization, body composition amongst other components. The diet considers food as a drug. ...

Introduction

CD4 is a cellular immune response co-receptor that raises the avidity of relationship between an antigen-presenting cell and a T-cell through interaction with non-polymorphic parts of the complex between T-cell receptor and major histocompatibility class II molecules. It directly contributes to transduction of signal via cytoplasmic relationship with the lymphocyte kinase. CD4 also plays a role as the high-affinity receptor for attachment of cells as well as entrance of HIV. The CD4 extracellular part consists of domains that resemble immunoglobulin D1 to D4. This component of the CD4 has been qualified as a recombinant soluble protein, and crystal structures have been identified for ...

The human body is constituted in about 20% by protein. Proteins, on their hand, are constituted by amino acids. Therefore, the intake of proteic food is very important so that the proteins of such foods may be metabolized and the amino acids and energy gathered to maintain the body’s protein levels.

The existence of correct amounts of amino acids in the body is essential for several functions:

- the body’s skeletal muscle constitution; - for transport of nutrients to the same cells; - cell repair; - cell communication: - influence on organs’ good function; - eliminating residues from metabolism; - amino acids are also nutrients in itself, ...

Severe acute respiratory syndrome is a life threatening condition that is caused by a virus from the Coronaviridae family. The SARS virus is believed to have originated from the Guangdong province of southern China before spreading to Hong Kong, Canada and the United States of America. The virus has an incubation period of 2 to 7 days but it may last up to 10 days. Like other respiratory disease, SARS is spread through direct contact; long range and short range. The major agent of transmission is the droplet nuclei. The symptoms of the disease include: fever, chills, malaise, cough, dyspnea and ...

It was advised that i stat home and whenever it is important to eat out, the 'Eating Smart Restaurant Guide' should be my companion.

1 .Reduction in quantity of processed sugar, saturated fat and trans- fat consumed. 2 Increased consumption of vegetables. 3 I will have to increase the number of times I eat at home and reduce my visits to the restaurants. The acceptable macronutrient distribution range for carbohydrates in adults is 45-65%, while for proteins it is 10-35% of the calories taken daily by the person. The acceptable macronutrient distribution range for fats is ...

Introduction

Liver is an important organ in the digestive system. The selected infection is hepatitis C. This viral infection affects the liver and then results in the liver enlargement. This form of hepatitis is one of the major challenges worldwide since its discovery and this could be attributed to the absence of a vaccine against the viral infection. Another important contributory factor is that of the prolong period of inactivity of the virus within the host before clinical manifestation. These all contribute to the level of destruction of the liver caused by the virus.

Etiology

The main aetiology is by the hepatitis ...

Abstract

Factor V Leiden is a genetic mutation that confers resistance to the action of activated protein C and thus predisposes to venous thromboembolism. It’s inherited as an autosomal dominant trait and is prevalent amongst Caucasians with a carrier rate of 4%. Deep venous thrombosis and pulmonary embolism are the most common clinical manifestations of FVL related VTE although it can also affect other sites. Genetic testing for FVL is used for diagnostic as well as predictive purposes. Diagnosis of FVL is based on protein assays and

DNA analysis technology. Familial genetic testing for FVL is generally not recommended except in ...

My current eating habits include approximately 2600 calories a day, based on what I entered into my Food Diary CheckPoint. Within my diet, I often eat vegetables whenever I think to, including bell peppers, onions, carrots and the like. I often have meat with many meals, including lean chicken breast and ground beef, sometimes ground pork. White rice is a substantial starch I find in my meals. My soda intake consists of almost strictly diet sodas, but they are still a nutrient lacking food that I should restrict. More nutrient-rich foods I occasionally eat include skim milk and Greek yogurt, as well ...

Protein synthesis is the process through which the biological cells produce new protein molecules. The process helps in balancing the cellular proteins that are lost through export or degradation. The synthesis of proteins occurs through a series of processes known as translation where the ribosomes are the organelles that are involved in the creation of proteins. Translation is a part of gene expression using the messenger RNA or mRNA that is produced through transcription of the DNA molecule. The produced mRNA is then decoded to produce a chain of specific amino acids using the ribosome complex. The amino acid chain produced is ...

A 36-year-old woman is married and has two adopted children. She is diagnosed with polycystic kidney disease (PKD), and she will soon have to go on dialysis or consent to a renal transplant. 1. What additional data should the nurse collect to help the patient make the best decision for herself? The nurse would need to collect previous health records of the patient. Gathering information on the cause of kidney failure and the medications used would help the nurse analyze the health issue in a precise manner. Gathering information on the patient’s psychological health and family would help ...

Marketing Plan for Coca Cola plus protein

INTRODUCTION Coca Cola is one of the biggest players of carbonated and non carbonated soft drink market in the world. It is one of the most valuable and iconic brands in the world; Forbes has listed Coca Cola as the world’s third most valuable brand (World’s Most Valuable Brands, 2014). It is one of those companies that invest heavily in its marketing initiatives. In 2013, the marketing spend of the company stood at $4 billion (Team Trevis, 2014). Coca Cola is a market leader of the soft drinks market and has a brand portfolio that included drinks like ...

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs. Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

Re-uptake involves the reabsorption of neurotransmitters by any of the neurotransmitter receptors or transporters. The transporters involved are usually of pre-synaptic neurons. Re-uptake occurs after the neurotransmitter has transmitted a neural impulse. Re-uptake is very crucial in order for the normal functioning of synapses to take place. This is because it allows the neurotransmitters to be recycled, thereby regulating their levels in the synapse. This in turn dictates the length of time it takes for a signal - generated by neurotransmitter release - to last. Neurotransmitters are usually large hydrophilic molecules that cannot pass through the neuronal membranes. For this reason, ...

- The stromal cells that are found in the developing gonads of tetrapods begin from the medulla from the peritoneal wall and the cortex from inter-renal blastema or mesonephric. Hence the gonads have a dual origin. - Spermatogenesis occurs in a syncytial complex because it involves the formation of sperm from undifferentiated cells that are first attached to rachis. Syncytial cell complex or syncytium result when cells with single nuclei fuse to for a cell with nucleus. Syncytial cell complex is vital in oogenesis in that it assists in the differentiation of cells. One other developmental model is vitellogenesis. - ...

Pasteurization is a process that involves heating of raw milk to a certain temperature within a given set of time. This process kills harmful bacteria found in unpasteurized milk, for example, Salmonella, Listeria and E coli responsible for food borne illnesses. These bacterial organisms are responsible for human diseases such as Typhoid, diphtheria, Listeriosis and tuberculosis. The process was first initiated by Louis Pasteur in the year 1864. Pasteurized milk can stay for a certain period without refrigeration. However, refrigeration especially after opening the seal, is still necessary because of some nonpathogenic bacteria that because food spoilage and is non-receptive for heating. ...

Consumption of 17g of arginine for two weeks has been found to enhance collagen production, according to a recent study (Crowe & Brockbank, 2009). But, what is a wound? Brown (2013) suggests that wounds are injuries on tissues or the skin, and are prominent in bony areas. Various factors contribute to the development of wounds, but this paper will evaluate the role of calorie malnutrition. This paper examines how high calorie diet promotes wound healing. Enteral and oral nutritional support is of vital significance according to Crowe and Brockbank (2009). A randomized study that was centered on arginine analyzed how nutritional ...

Aim: To separate Green Fluorescent Protein (GFP) using poly-acrylamide gel electrophoresis (PAGE).

Introduction: Bioluminescence observed in many marine organisms is due to a protein called as Green Fluorescent Protein (GFP). Since its discovery, GFP has been useful in biotechnology due to its versatility and ease of transformation in E. coli (Chalfie et al., 1994). GFP is a 26.87 kilodaltons (kD) protein composed of 239 amino acids. It has a barrel structure with a fluorescent chromophore embedded deep in the interior of the protein. The chromophores in GFP are excited at a wavelength of 395 nm, and when the electrons of chromophores drop to a lower energy state they emit fluorescent light of 509 nm (Ormo et al., ...

Connective tissue growth factor induces collagen I expression in human lung fibroblasts through the Rac1/MLK3/JNK/AP-1 pathway

Many lung inflammatory diseases present pulmonary fibrosis as one of the symptoms, which is characterized by shortness of breath, chronic coughing and discomfort in the chest. It causes modifications of the lung tissues due to overgrowth, hardening and scarring. This occurs mainly due to deposition of collagen, an extracellular matrix (ECM) protein, whose turnover is regulated by fibroblast. Fibroblast is an important cell regulator that is enlisted by a site of injury. This process is driven by release of transforming growth factor-β (TGF-β), platelet-derived growth factor, interleukin-6 (IL-6), and IL-8/CXCL8. Previously, fibroblast was thought to express no or very ...

There are more than 5000 genetic disorders up to date. These genetic disorders are most evident at birth or during the onset of early childhood. This results to admission in different programs in rehabilitation centers, school programs in order to help the victims affected by such conditions. These disorders can primarily affect the body system, muscles and the respiratory system. One such disorder is the Rett syndrome. Rett Syndrome is a phenotypically distinct progressive X-linked dominant neurodevelopmental disorder that affects females (Volkmar et al, 2007). There are different types of Rett syndrome. These include the classical Rett syndrome, provisional ...

Recorded intake of macronutrient. Based on my daily intake of recorded foods, foods that provided proteins include chicken, beans, dairy (including yoghurt and milk), egg, bread etc. Carbohydrates were provided by bread, pasta, cereal, dairy, beans and fruits. Lipids or fats were provided by chicken and dairy, eggs and the oil that was used in preparation of food. Based on the three day recorded protein intake the amount of carbohydrate and protein (approximately 55 gm protein and approximately 180-200 gm carbohydrates) compared well with the recommended dietary reference intake for an adult. However the level of lipids or ...

Digestion occurs in the gastrointestinal system, which is composed of the gastrointestinal tract (GI tract) and the associated accessory organs.The process of digestion occurs through six steps, namely ingestion, secretion, mixing,digestion, absorption and defecation. (Tortora&Derrickson, 2008).

Anatomy of the digestive system

The GI tract or the alimentary canal is a long winding tube that measures approximately 30 feet (9 meters) in length. The mouth, esophagus, pharynx, stomach, small intestine, large intestine and the anus make up the GI tract.The salivary glands, tongue, teeth, gallbladder, pancreas and the liver make up the associated accessory organs (Tortora&Derrickson, 2008). The mouth. The mouth starts with the ...

- Cell Culture and Cell Lines: Three breast cancer cell lines: CAL120, EVSAT and MDA-MB-231, were cultured individually, in RRMI 1640 supplemented with 20% Fetal Bovine Serum (FBS), 100U/ml Penicillin, 100U/ml Streptomycin and L-Glutamine. The cells were cultured and maintained in an incubator at 370C supplied with 5% CO2. For subsequent experiments, the cells were further cultured till confluence, in 6 well and 96 well plates using RPMI+20%FBS. 1.2 Treatment of Cells: For treatment, 1x106 cells were seeded in 6 well culture plates, and incubated for 24 hrs. After 24 hours, the old media ...

With reference to Biological studies, homology is the presence of shared pedigree between living organisms in terms of evolution. The homology might be in terms of genetical constitution, or physical constitution, to name a few. Here, I have described the evolutionary homology between various phyla supporting the description with a phylogenetic tree.

Genetic code is the combination of DNA and RNA sequences that constitute the amino acid sequences used in the synthesis of an organism's proteins. It is the biochemical basis of heredity and generally, protein coding amino acids is very similar in all eukaryotes and prokaryotes. The principle differences occur ...

Introduction

The study explored the homological characteristics of 22 species. The organisms were human, pygmy chimpanzee, common chimpanzee, gorilla, orangutan, white-handed gibbon, macaque (Cercopithecine monkey), horse, bison, cat, dog, bird, bat, frog, rattlesnake, alligator, fish, whale, wolf, pig, bacteria, and cow. The species were categorized as mammals, reptiles, birds, fish, or amphibians. However, humans and apes were further classified as primates. Moreover, the research sought to determine whether the species share characteristics that link them to a common ancestor. The research hypothesis was that if the evolutionary path of the 22 organisms were traced, then a common ancestor would be observed.

Universal Genetic Code

The ...

Introduction

One of the most important part of protein assay is the determination of protein concentration. There are diverse methods used for protein concentration determination, but the most widely used are the spectroscopic methods of analysis or spectroscopy. Spectroscopy usually involves the determination between the correlations of the degree of interaction of light with the analyte, which is the proteins in this particular experiment. There are different types light to analyte interactions and one of these is the absorbance of light or electromagnetic radiation by the analyte. This type of spectrometry is called absorbance spectrometry.

Principle involved in absorbance spectrometry and spectrometers

The majority of substances absorb ...

Human Immunodeficiency Virus, a main cause of worldwide AIDs, belongs to the Lentivirus genus of Retroviridae family. Pathogen causes chronic type of symptoms, taking a long time of latency it constantly keeps replicating inside human body. HIV is categorised into two types, HIV-1 and HIV-2. The genome of this retrovirus consists of two single-stranded RNA molecules. Though, both are involved in AIDS, HIV-2 seemed less virulent and demonstrated more frequent connections with the disease of central nervous system (Fanales-Belasio et al. 5). Like other retroviruses, the genes located on HIV chromosome are gag, pol, env. The functions of gag, pol ...

Obesity refers to the condition of having more than average fatness that is different from being overweight, which is the condition of having too much weight. This increase in weight mostly comes from bone, muscle, fat, and body water. Obesity is a global problem, and its prevalence has approximately doubled between 1980 and 2008. Nearly, 2.8 million people die annually due to the problem of being overweight or obese (World Health Organization (WHO), (n.d.). Australia has reportedly highest rates of obesity in the world. Nearly quarter of the children in Australia and 63% of the adult population are overweight as ...

Summary

Introduction The two articles focus on researches that were conducted so as to get some viable findings that involved analyzing the effects of amino acids and carbohydrates of muscular adaptations under different circumstances. Summary In the research by Ferreiraa et al. (2014), the most beneficial strategy of improving the performance of muscles is through the intake of nutrients, especially carbohydrates and protein during peri-exercise. Several methods and materials were used before coming to this conclusion. The methods used were supplementation protocol, experimentation approach and pretesting analysis. Resistance exercise protocol, muscle biopsies and blood sampling were highly ...

Abstract

Human XPA is a protein engaged in DNA damage recognition and repair. A mutation in the gene encoding the protein causes an autosomal recessive disorder, xeroderma pigmentosum (XP), which renders an individual sensitive to sunlight and causes skin cancer. XPA is a 273 amino acid protein, which is predominantly localized in the nucleus. It is expressed at both high and moderate levels in several tissues. The protein serves as a damage sensor in a nucleotide excision repair mechanism, through which the damage to a DNA strand is verified and repaired by formation of a newly synthesized strand. Besides this primary NER mechanism, XPA in conjunction ...

Membrane transport refers to the mechanisms by which ions and other small molecules move across the lipid bilayer of the plasma membrane. The lipid bilayer of the plasma membrane allows the passage of certain molecules down their concentration gradient. This passage depends on the charge, size and polarity of the molecule. Non-polar molecules and small polar molecules diffuse easily across the cell membrane by the process of simple diffusion. However, it becomes difficult for charged polar molecules to enter the cell even though the concentration of the ion is more outside the cell when compared to the cytosol. At ...

Harvey requires enough energy to perform. Consequently, he must consume enough calories to meet all his energy needs. The actual requirement for calorie intake depends on one’s level of fitness and bodyweight. In addition, the intensity, frequency, and duration of physical activity also greatly influence one’s actual energy requirement.

Carbohydrates

Given that Harvey’s daily workouts involve high-intensity activities, his calorie intake should comprise of between55 to 70 percent carbohydrates. In this case, 3 grams per pound of body weight would be appropriate. Based on this recommendation, Harvey should consume approximately 480 grams of carbohydrates per day in ...

Introduction

Enzymes are proteins which have the ability to facilitate metabolic reactions without being used up in the reaction. The enzyme amylase is responsible for facilitating the hydrolysis of starch to form sugars that are then assimilated by the body. Amylase is an enzyme needed for the digestion of carbohydrates for nutritional purposes. Starch is one of the most important sources of energy in the body. Starch is a polymer made up of several monosaccharide and disaccharide monomers. However, starch cannot be absorbed and used in the body as it is (Cornish-Bowden, 134). Consequently, it has to be digested into smaller ...

Introduction

Cells are independent units of life. However, when a cell becomes part of an organism, it becomes part of a tissue and organ system. Cells in a tissue are joined to each other and to the extracellular matrix (ECM) by cell junctions. There are three kinds of cell junctions: Occluding junctions, anchoring junctions and communication junctions. Occluding junctions occur in the epithelium where the adjacent cells are so tightly sealed together that even small molecules cannot pass. Communicating junctions allow the exchange of chemical and electrical signals between cells. Anchoring junctions are the ones which attach a cell either to its neighbor or ...

The immune system has an immune arsenal that help in the combat of infections including the challenging viral infections (Iannello 2006). Upon infection by a virus, the host invariably reacts by activating its line of defenses. These immune defenses can fall into two main groups: the innate immune system (first line of defense) and adaptive immune response. The innate immune system is non-specific in nature, but the adaptive immune system is acquired following previous infections or vaccinations (Iannello 2006). However, viruses continue to undergo an evolution and have engineered strategies that evade the hosts’ immune antiviral responses. This paper examines ...

Introduction:

In recent times, there has been a rapid growth in the amount of data generated and collected on the human genome project and sequencing projects in other organisms. The rapid growth of biological data has consequently led to increased demand for data analysis and interpretation methods and tools. The evolving science of bioinformatics provides the necessary processes and tools to conduct this data analysis (Bayat, 2002). There are a number of specifically dedicated bioinformatics problem-solving tools and successful applications. However, despite the many existing applications, bioinformatics is still considered as a science in its infancy, and the process of massive integrating it ...

Abstract

Adequate nutrition is required for optimal wound healing. The normal processes that allow the progress of wound healing are disrupted due to nutritional deficiencies. Increased infection rates and decreased wound tensile strength are associated with malnutrition. Chronic on-healing wounds are formed in malnourished patients or individuals. Such patients can develop infections, pressure ulcers, and delayed wound healing. Morbidity and mortality among malnourished patients with chronic wounds constitute a serious clinical concern. Wound healing is considered as a complex process. It is the process of replacing injured tissue with new tissue (produced by the body). Production of new tissue is associated with ...

Organization:

The term biotechnology refers to any process that involves biology and technology. Though the term was coined as far back as 1919 by Karl Ereky (Verma et al., 2011), biotechnology was established as a field of applied science only in the late twentieth century with the advent of recombinant DNA technology. Recombinant DNA technology is the generation and application of recombinant DNA molecules or rDNA. In a nutshell, recombinant DNA technology involved the cutting of a fragment of DNA and its insertion into a specific circular DNA molecule called a vector. The DNA is cut by enzymes called restriction endonucleases, and joined to a vector ...

(Author)

Regulation of Xiap Protein in Breast Cancer Breast Cancer Breast cancer is the type of cancer that develops in the tissues of the breast. Two most commonly reported types of breast cancer are “ductal carcinoma” and “lobular carcinoma”. Ductal carcinoma is initiated in the lining of the milk ducts, which are thin tubes involved in carrying of milk from lobules of the breast to the nipple. Ductal carcinoma can be in the form of Invasive ductal carcinoma (IDC) as well as Ductal carcinoma in situ (DCIS). IDC is the most commonly found type of breast cancer ...

- Describe the role of the following in protein synthesis - DNA, nucleotides, RNA, ribosomes, rough ER, and Golgi apparatus. DNA or deoxyribonucleic acid is a double stranded hereditary molecule containing the genetic code from which specific protein molecules are synthesized. First step in protein synthesis is synthesis of single stranded complementary RNA. RNA also contains all the components of DNA except a ribose sugar. Special RNA called messenger RNA (mRNA) carries the message to ribosomes. Under mRNA’s direction, transfer RNA (tRNA) delivers amino acids to ribosomes and ribosomal RNA (rRNA) attaches each amino acid to form the final ...

Introduction

As a multi-functional protein, Cytoplasmic Actin can be found in all eukaryotic cells at higher concentration levels of over 100 μM. It is the most abundant protein found in most eukaryotic cells, which makes it a critical player in different cellular functions, such as maintaining cell shape and polarity to transcription regulation. The structure of Cytoplasmic Actin is distinguished by a large number of polymorphic proteins which form the filaments. In eukaryotic organisms, such as archaea and eubacteria, actin-like proteins activities are related to sub-cellular organization, motility, cell shape maintenance and cell cycle progression; they are either associated to cell membrane or ...

Introduction

Bacteria are the very first life forms in the history of our planet. Since the beginning they were exposed to extreme changes of temperature, pressure, pH and nutrients; hence, to survive, they had to adapt themselves. One strategy of survival is to go into suspension or dormancy, by converting themselves into a seed form or spores. When conditions become normal, these spores germinate back to vegetative form. This property is also seen in pathogenic bacteria that infect higher animals such as humans. One such group of bacteria is from the Genus Bacillus.When exposed to stress, these bacteria go into dormancy ...

Structure and Functions of Cytoplasmic Actin

Cytoplasmic actins refers to the conserved proteins. Actin can be found in all eukaryotic cells, and are involved in variety of cell motility. Protein structure is formed by combination of variety of amino acids by losing the molecule of water. Proteins get involved into variety of biological functions, and proteins have different types of structures depending upon the requirements. This paper intends to discuss different types of protein structures, their functions and other related aspects. The structure of proteins can be divided into three categories i.e. primary, secondary and tertiary. The structures of proteins are polypeptides-sequences that get ...