Essays on Chromatography

Questions to Answers

- What is the difference between Active and passive sampling?
Active sampling is different from passive sampling in that it employs an air sampling pump that serves to actively pull air and pass it through a collection device, for instance a filter. On the other hand, passive sampling does not need active air movement, like the one generated by a pump. Instead, vapors and airborne gases are collected through a physical process including permeating via a membrane or diffusion through a layer of static air.
- What is the difference between Instantaneous (real-time) and integrated (continuous) monitoring?
Instantaneous monitoring

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Instrumental Analysis

Mephedrone molecular structure
Nephyrone molecular structure
There are several structural differences between the three chemical substances that make adrenalin distinguishable from both Mephedrone and Nephyrone. The adrenalin molecular structure has a more stable structure more than both Mephedrone and Nephyrone. The adrenalin structure has an extra OH molecule that makes it distinguishable from the Mephedrone and Nephyrone. Several analytical tests can be used to determine this difference.
There are various chromatographic techniques that can be used to test the purity of the adrenalin tablet. The first method is paper chromatography. This chromatographic method is used to analyze chemicals that

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Introduction

TLC or Thin-layer chromatography refers to a technique that is applied in the separation of mixtures that are not volatile (Reich & Schibli, 2007). The process is done on a sheet of plastic, glass, or aluminum foil that has been coated using a thin layer of material that is adsorbent such as aluminum oxide, silica gel and cellulose (Sherma & Fried, 2003; University of Colorado, 2014). This adsorbent layer is referred to as the stationary phase. The sample is usually applied using a capillary tube on the plate, and the sample is taken up the

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Chromatography is a laboratory technique which involves the partitioning of molecules of plant pigments. In this technique a mixture is dissolve in a fluid which carries the unknown analyte to a structure which can hold the material causing the two components to separate. This technique can be categorized as preparative or analytical. By preparative chromatography it means, the separation of a mixture for the purpose of advance utility in this case, the mixture undergoes a purification process. Analytical chromatography on the other hand is prepared in smaller doses of materials and is performed casually to measure the relative proportions

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Identification of Codeine by Gas Chromatography – Mass Spectrometry (GC-MS)

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Aim
The present practical exercise aims at examining a commercial Panadeine tablet for the presence of codeine and paracetamol using GC-MS.
Introduction
Methyl morphine, codeine, occurs in opium at low concentrations. Codeine is a pro-drug because it undergoes metabolism or demethylation, in vivo, to form the active analgesic morphine. The experiment 3 involves a pH dependent extraction technique. The process separates the active constituents of Panadeine. Before extracting Panadeine, however, Eosin B undergoes extraction in order to demonstrate the effect of pH on the solubility of the compound. The GC-MC allows for the separation of a mixture

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Purpose

The experiment aims to identify two unknown amino acids using paper chromatography. To be specific, the retention factor or Rf values of standard amino acids was used to see which one matched that of the unknowns.

Introduction

Chromatography can be defined as the separation of a mixture into various fractions by distribution between a mobile phase, which can be a gas or a liquid, and a stationary phase, a liquid or solid. Different substances have different affinities for the stationary and mobile phases. A substance that has no affinity for the stationary phase will remain in the mobile phase and

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Introduction

Chromatography is a term that refers to the separation of chemicals in a mixture so that they may be used or detected separately. Gas chromatography involves separation of compounds with comparatively low boiling points for easy detection in complex mixtures. The separation compound is based on their variations in vapor pressures and level of affinity to solid material at the test instrument. Since the vapor pressure of each compound depends on the intermolecular forces pulling molecules together, gas chromatography utilizes any of the properties of matter. In this technique, a sample of test chemical is injected through a syringe

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Abstract

Instance of countries introducing restrictions on the amount of harmful gases released to the atmosphere is on the rise. Countries like the United States have introduced policies which govern motor vehicle pollution. Gasoline is the main component of debate. As a result several studies have been done to determine the components of gasoline. In this experiment, components of gasoline are determine by an analysis process called gas chromatogram.
Samples of gasoline used in the experiment are collected from four stations BP, Marathon, Moto-Mart and Sunoco from Ashland. The samples are then analyzed using a gas chromatogram. 10 ml was

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Dihydrofolatereductase (DHFR) is an important enzyme in the formation of DNA (Goodsell, 2002). DHFR controls the amount of tetrahydrofolate in the cells, which in turn is an important cofactor for purine and thymidylate synthesis and ultimately in the growth and proliferation of the cell (Davidson &Sittman, 1999). The enzyme is common to many species on earth. Human and bacterial DHFR have a relatively low rate of primary sequence agreement at 26%. However, they both retain a common general structure.The key role that the enzyme plays in biology as well as the variations that the enzyme displays makes it an

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Introduction to Use of Silica in Sampling of Plant Compounds

Liquid chromatography is the most widely accepted and fundamental methodology for the separation and sampling of plant compounds. Liquid chromatography has the abilities to separate and sample a huge variety and diverse range of organic compounds ranging from proteins and peptides to small molecule protein drugs. The liquid chromatography is a technique that works by placing or injecting a minute volume of liquid sample into a silicon tube which is packed with porous substances or particles. This phase is called the stationary phase. The next phase is the extraction phase where the individual compounds present in the sample are

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- Purpose
Paper chromatography is a technique which is used to distinguish between the various components of a mixture. It helps to separate out the components of a solution and identify individual components. In Paper chromatography, filter paper is used as the primary material for conducting the experiment.
- Procedure
For the experiment, a beaker of 1L capacity is taken and filled with 100mL solvent. The beaker is known as chromatogram tank. Next, a filter paper around 12 inches long and 9 inches wide is taken. A light line which is 2cm apart from the bottom of the paper

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