Essays on Vitro

Cell culture is an in-vitro technique of cultivating cells under controlled conditions and in sterile conditions. The technique was first developed by Ross Harrison in 1907. During the process of cell culture, the cells are detached from their tissue of origin and segregated from each other, either by mechanical or enzymatic means. The detached cells are then transferred before culturing them in cell culture plate or flask. The first generation of producing cell through this method is called primary cell culture. The cells from the primary culture are transferred to a new culture plate with a fresh medium to ...

O'Neill, K. E., Senapati, S., & Dokras, A. (2015). Use of gonadotropin-releasing hormone agonist trigger during in vitro fertilization is associated with similar endocrine profiles and oocyte measures in women with and without polycystic ovary syndrome. Fertility and sterility, 103(1), 264-269.

Comprehension

The comprehension phase of the quantitative critique process is characterized by the identification of the main terms and concepts of a quantitative study (Grove, Burns & Gray, 2014). The steps of the research process are also identified at this phase, including the purpose, design, methodology, analysis, and results. The comprehension phase of quantitative critique process was achieved by reading ...

Abstract

The field of Nanoscience has evolved due to the other advancements in the other fields of study such as the Nanotechnology. While the latter is a subset of the former, there is credible evidence that points to the fact that Nanotechnology has been developed thanks to various areas such as biology, material science, engineering physics, chemistry and much more. It is the foundation on which the Magnetic Nanoparticles have been embedded. MNP is nanoparticle that has the magnetic manipulation ability. Thus, this paper focuses on the study of the Magnetic Nanoparticles especially how they are formed or synthesized and ...

The concepts presented in the film, Gattaca are not entirely fictional. Advances in science and technology have presented some exceptional cures and treatments for the human species. There are numerous discoveries and inventions that have made the quality of one’s life better than what was once possible. However, along with the numerous phenomenal breakthroughs in medicine, there are also some developments that come into question as unethical. The premise of the movie was structured around genetic engineering of embryos’ to be designed into the perfect human based on the requests of the parents-to-be. The main character Vincent was ...

Synthetic biology is the latest trend that integrates biology with engineering. It deals with the making of biological molecules or systems that does not natural in the biological world. These molecules or system that are constructed, help to complement natural biological systems and help in performing particular biological task (Khalil and Collins 367-379). A simple example of synthetic biology would be manipulation of bacteria through recombinant technologies to produce recombinant protein. A vast number of applications are possible with synthetic biology. With the advancement that are currently happening in the area of bioinformatics, it is possible to reprogram human ...

The website explains in detail how the drug development takes place and how investments made in pharmaceutical companies are affected by this process. The website begins by asserting that there is higher probability of getting 12 male children in a row than a drug seeing the shelf of a pharmacy (Williams par. 1). With that noted, it is therefore imperative for an investor in the health sector to understand the dynamic of drug-manufacturing. The initial stage is the drug discovery which involves identify a molecule e.g. a protein or gene that participates in a disease (Williams par. 2). If ...

In global societies, discussions concerning the use of animals in medical tests for research are uncomfortable and controversial. Modern technology often broadcasts horrific photos of procedures being conducted on helpless creatures accompanied by claims of untrustworthy results and skewed statistics. In the long run, animals will continue to be used for testing in laboratories until the value of the practice is diminished. “The benefits of animal research have been enormous and it would have severe consequences for public health and medical research if it were abandoned “ (Festing and Wilkinson, n.p.). This paper will present information regarding whether animal testing ...

Average velocity

The average velocity reported for microtubules propelled by kinesin expressed in insect systems is much higher than that reported for kinesin expressed in E. coli with a calculated mean and median of approximately 2.9330μm/s and 2.7974μm/s respectively for kinesin expressed in insect systems and 1.1579μm/s and 1.0903μm/s respectively for kinesin expressed in E. coli. This result is similar to result obtained by Korten et al. (15: 64) and Agayan et al. (29: 2265–2272) and the lower velocity observed in microtubules propelled by kinesin expressed in E. coli is likely due to the presence of ...

Please insert your name here

Please insert your professor’s name here Please insert course number here Introduction Kinesins are proteins which serve as molecular motors for the directional transport of various cargos (Hirokawa et al. 10: 682-696). They perform this important function using different mechanisms to recognize, bind, determine the direction of transport and unload the cargos with an energy efficiency which can exceed 50%, thereby equipping them to play important roles intracellularly in the transport of organelles and vesicles, in biosensing as well as in the development of other bioengineering solutions (Agarwal 11; Hirokawa and Takemura 301: 50–9; Hirokawa et al. ...

Introduction

The large production of bisphenol A (BPA) in the world has been documented because of the increased usage of the same in various industries. The most critical industries that have been using this BPA include those manufacturing containers for foods and beverages, cigarette filters, thermal receipts as well as paper currency. With such a huge provision, it is critical that the basis of such developments only emanates from making the right choices over its uses because its production to a total of 2.7 million tons could be disastrous to the body. The main reasoning here is that the chemical ...

Stem cell research and therapy are not new and continue to be a controversial topic. The benefits of stem cells, and what most scientists agree are the potential benefits of stem cells, in curing disease and regenerating tissue are seemingly limitless. In the past bone marrow transplants were the primary method used to obtain stem cells for research into cures for contemporary diseases such as cancer, heart disease, Parkinson’s, Alzheimers, multiple sclerosis, and diabetes as well as spinal cord injuries. However, Embryonic Stem Cell Research (ESCR) is much more controversial. There has been a recent shift to use ...

Abstract:

This research aimed at determining the ethical issues that surround surrogacy. Surrogacy being an issue that has elicited much debate is gaining traction because of the many childless couples out there that either suffer from infertility or are involved in same sex marriages (Gunputh & Choong, 2015) It both has the positive and negative which have been explored in this research. The research involved going through already existing information provided in accredited books, journals and websites. The research first explores the information that surrounds the positive side of surrogacy before delving into the negative side of the issue. Further reviews ...

The activity of AMPK supports the normal function of the endothelial barrier (Xing, Wang, Coughlan, Viollet, Moriasi, & Zou, 2013), while on the other hand, the exposure of LPS ensures that there is inhibition of AMPK, thus accentuating lung injury and endothelial barrier dysfunction (Kratzer et al., 2012). It is a fact that LPS increases the permeability of the endothelium, which occurs once there is a decrease in the AMPK activity. This is consistent with the fact that the activation of AMPK activator, AICAR, emphasizes LP, once it is applied in vitro to test the hyper endothelial permeability. Experiments done ...

Abstract:

This research was aimed at determining the ethical issues that surround surrogacy. Surrogacy being an issue that has elicited much debate is gaining traction because of the many childless couples out there that either suffer from infertility or are involved in same sex marriages. It both has the positive and negative which have been explored in this research. The research involved going through already existing information provided in accredited books, journals and websites. The research first explores the information that surrounds the positive side of surrogacy before delving into the negative side of the issue. Further reviews of these ...

{Author Name [first-name middle-name-initials last-name]} {Institution Affiliation [name of Author’s institute]}

Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

Cloning is the process that a cell or an organism is produced asexually; meaning that no sex is involved in their formation and the product is identical to a specific ancestor. Animal cells are cloned simply because they can be produced from one fertilized cell and they are also genetically identical. In simpler terms, cloning is the process of producing many duplicate cells of an organism. In some cases, cloning is a natural process shown bacteria which are single celled, as well as identical twins in human beings and mammals. The process is also made possible to the animals ...

In the Society of Toxicology’s (SOT) public policy statement, toxicology is defined as the study of the interaction of chemical substances with living tissue in the context of the following issues: how they affect a biological organism’s processes, how to reduce the chemical substance’s potential harm to the organism, and what are safe levels of exposure (Society of Toxicology, 1999). The purpose of this paper is to evaluate the SOT’s position statement on animal testing. The topics addressed in the paper are how toxicity levels are determined, the application of SOT principles to course material, ...

The recent developments in technological advancements and scientific breakthroughs have presented modern ways of creating new things and doing things. One such development relates to the introduction of technology-based medical procedures, which have made it possible to achieve feats that yesteryear medical scientists could only dream about. The field of medicine has shown tremendous improvement as a result of the incorporation of new technological meth-ods into the field of medicine. Genetic engineering procedures have become common not only in the creation of medicines and vaccines but also in disease management before and after birth. Scientists have come up with ...

Article #1:

The article focussed on the breeding behaviours of emperor penguins that are recently discovered with the help of satellites. Mostly it is believed that penguins lay their eggs on sea ice that is fast and stable and that remains intact under the chick fledge. Fretwell and co-workers conducted this study on forty-six colonies emperor penguins. The recent observation revealed that few colonies of emperor penguins breed on ice-shelves instead of sea ice. Due to rapid climate changes and the incidence of ice melting the penguin population has declined and very close to extinction. It is a big concern that ...

Genes are the hereditary units that express proteins in a cell to carry out body metabolism and overall growth and survival. They are the functional units on DNA that is packages inside the nucleus within chromosomes. Humans consist of 23 pairs of chromosomes where 22 are autosomes and one is sex chromosome. These chromosomes have been studied to comprise of around 25,000 protein coding genes. There are continuous phenomenon of changes and aberrations occurring in bases and chromosomes that alter its composition and sequences. These changes or mutations can be harmful if it modifies the gene information as ...

{Author Name} {Institution Affiliation} {Date}

Introduction

Genome imprinting is the latest technique that has genetically modified the face of infertility; it is an epigenetic phenomenon through which the parental origin of a gene is determined based on its expression. 1 There are a vast number of genes in mammals that are marked with their parental origin, and the result of this phenomenon is a single parental allele. These marked genes are known as “imprinted.” They rely on the epigenetic machinery determining their parental identity and maintaining their origin-specific gene expression. There are 100 imprinted genes estimated in human and 200 ...

Introduction

Nanotechnology is a new venture for engineering field and has been seen as a breakthrough regarding materials thus providing a greater opportunity in enhancing both innovations as well as discovery in engineering and science fields. The primary application within the engineering sector have been; to create room for new nanofabrication technologies to improve nanostructures designing and construction which have by-passed the current technology. Additionally, it provides a chance to apply nanostructures in various devices such as magnets, optics, electronics and biotechnology devices (Dvir et al., 13-22). The opportunity is significant because the technology enhances innovation of materials as well ...

Introduction

The total number of patients with cancer keeps soaring globally with around 14 million cases diagnosed by 2014. Each year, about 8 million people die of cancer-related illnesses globally. There are quite some remedies that have been proposed to mitigate in the cancer menace. This study is focused on cancer treatment by curcumin through tyrosine kinase inhibition. Curcumin is the primary coloring agent found in turmeric, a tuber of the herb Curcuma longa Lin. It has widespread use as a flavoring agent (Aggarwal and Bokyung 87). It is also well known for its medicinal value commonly used to suppress ...

Is this an appropriate reason to become pregnant?

Advance in technology can often raise issues regarding their application in everyday life. There are various moral considerations that arise in association with the ability to choose specific features of children when they are born. The advent of in vitro fertilization (IVF) has given parents the opportunity to decide specific characteristics of their children at an early stage. The underlying issue of the case is whether or not they should have a child that is matched with their sick child's tissue type so that they can obtain a transplant. While this would be of great medical benefit it presents ...

Introduction

Quality is the characteristic of a service or a good to be fit for use by the customer or the consumer. The pharmaceutical quality means the production of a product that is free of contamination that gives the therapeutic benefit that is labeled in the container to the customer. This quality is either evaluated in vitro or vivo by the tests performed. In quality design, the vitro product is ensured to have good performance and the same provide a similar quality in the vivo performance test. In this case, the product performance is related to the product performance.

Quality by Design (QbD)

This ...

In Vitro Models of Skin for Topical and Systemic Drug Delivery Applications

The drug delivery is a significant process in medicine development. The development of topical or systemic drugs requires models that present the mechanism of the drug activity. Topical delivery of medicine has a long story of application. The advantages for the topical delivery are as follow: self-administration and simplicity of application, absence of side effects, high surface area for drugs absorption. However, the method has recently been recognized as far less efficient than the systemic drug delivery (oral or injectable) (Kulkarni, 2009). The paper presents advances in understanding the drug delivery using in-vitro models.

Topical Drug Delivery Models

The artificial in-vitro models are ...

Immunogenic cell death: the role of SAGE

Immunogenic cell death: the role of SAGE

Introduction

Millions of cells are always being removed in the human body not only to regulate and coordinate the body’s biochemical pathways but also to keep the cells healthy. This particular systematic removal of cells occurs through a selective mechanism called cell death or apoptosis process. Apoptosis is not a new concept in the understanding of the biological workings of the body. This homeostatic death of body cells has been defined as a tolerogenic or a non-immunogenic event for many [1]. However, new discoveries have unearthed the existence of a different concept ...

Article Review

Synergistic effect of human plasma and antimicrobial peptidomimetics and antibiotics on pathogenic bacteria: The Application is a promising tool in enhancing the efficacy of future antibiotics. This work aims at summarizing and critiquing the Research in Microbiology journal research paper titled ‘Improved In vitro evaluation of novel antimicrobials: potential synergy between human plasma and antibacterial peptidomimetics, AMPs and antibiotics against human pathogenic bacteria’ .

Abstract

Condisering the hurdles of cytotoxicity and adverse side effects posed by existing antibiotics and the ever rising resistance of microorganisms to these compounds, there is a dire need for development of innovative antimicrobial peptidomimetics (AMPs) . Despite ...

Immunogenic Cell Death: The Role of RAGE

Introduction Millions of cells are always being removed in the human bodyin order to regulate biological pathways and keep cells healthy through a particular mechanism called cell death or apoptosis. Through apoptosis, such homeostatic death of cells has been defined as a tolerogenic or a non-immunogenic event for many years [1]. However, during the past few years, scientists have discovered the occurrence of a different concept of cell death – immunogenic cell death (ICD), where the death of a cell stimulates a response from the immune system against antigens of a dead cell, and this mechanism of cell death may ...

Bidens Pilosa is a perennial herb that is found to grow across the various temperate and tropical regions of the world. The plant is found to have extensive uses as a medicine and also as a food without any known side effects. The origin of the plant is in tropical America and is a slender erect annual herb that is found to grow up to heights of 1.5m.The plant is found to be covered with yellow flowers which produce black seeds in all directions. The plant is popularly referred to as Cobbler’s pegs generally grows well in moist ...

Introduction

Millions of cells are always being removed in the human body in order to regulate biological pathways and keep cells healthy through a particular mechanism called cell death or apoptosis. Through apoptosis, such homeostatic death of cells has been defined as a tolerogenic or a non-immunogenic event for many years [1]. However, during the past few years, scientists have discovered the occurrence of a different concept of cell death – immunogenic cell death (ICD), where the death of a cell stimulates a response from the immune system against antigens of a dead cell, and this mechanism of cell death may ...

Embryonic stem cells are produced from embryos, human or other animal embryos

.most of such cells have developed from embryos that develop from eggs which have been fertilized in vitro fertilization clinics. Such embryos have been donated for the purpose of research with the informed consent of the donors, and they are certainly not developed from eggs fertilised in a woman’s body. (Bethesda, 2015)

At the outset it has to be accepted that the embryonic stem cell research is a modern

day scientific marvel which can change the ways the various presently day incurable human ailments, are treated in the future. Diseases like cancer, Parkinson’s, Alzheimer’s, premature aging, stunted growth may altogether be eliminated in the distant future.

Moral and Ethical issues of Stem cell Research

However, there are moral and ethical issues ...

Specific Aims

Alpha Amylace (-Amylase) which is the most abundant enzyme in human saliva contributes to the formation of plaque (film of bacteria and mucus deposited on teeth that encourages the formation of dental caries). The alpha-amylase does this by acting as an ion or a molecule for the binding of Amylace-Binding Streptococci (ABS) to teeth. The aim of this project is to address the association of ABS on dental plaque which can be applied in future studies and clinical applications. The association will use quantitative real-time PCR (qRT-PCR) to determine the amount of ABS relative to the total bacteria in ...

Abstract

The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...

Executive Summary

The objective was to compare effects of GnRH antagonist and standard long protocol agonist on number, and the quality of Metaphase II oocytes, and on reducing risk of hyperstimulation, in patients who are affected by Polycystic ovarian syndrome. Polycystic ovarian syndrome is a common endocrinopathy of the women who are in reproductive age, which is a first cause of the anovulation. GnRH agonists are used in the IVF to avoid premature LH surge before the retrieval of oocyte. Research findings from this study can be significant to various Centers for Reproductive Medicine, and also to all the institutions that practice ART. ...

Full title- A COMPARISON OF THE INHIBITORY EFFECTS OF METAL IONS

Enzymes are an essential part for the functioning of the human system. Most of the enzymes require binding of metal ions and other non-protein components for their appropriate functioning. Such non proteins, which facilitate the functioning of enzymes, are called cofactors. The cofactors can be an organic cofactor as Thymine Pyrophosphate (TPP), Flavin Adenine Dinucleotide (FAD), Nicotinamide Adenine dinucleotide (NAD); or inorganic cofactors like metal ions, Mg2+, Cu2+, Mn2+, Zn2+ and Iron-sulphur clusters. When the enzyme is bound to a cofactor, ready to carry out its function, it is known as a ‘Holoenzyme.' An enzyme without a ...

Selection from an 8 year-period of pulmonary valve replacement and cross sectional follow up of a commercially available biological valve, assessed with established and a new method

What This Thesis Is About

Pulmonary valve replacement is a procedure necessary in many congenital heart defects and in-valve degeneration cases. Surgical results are excellent, but the longevity of artificial valves is very limited. Our goal was to give a retrospective overview of methods and results from the year 2000 until 2008 at Rikshospital, as well as the prospective five year follow-up of a biological valve made from bovine pericardium. Follow up included clinical examination with ECG, echocardiography, spirometry, treadmill testing, echocardiography ...

- Summary After a heart attack, the restoration of the cardiac function depends on the ability of the heart to generate cardiomyocytes, or new muscle cells. The ability is, however, weak and wanes with age. The alternative strategy involves delivering the newly grown cardiomyocytes to the damaged heart tissue. It requires the use of either induced pluripotent stem (iPS) or the embryonic stem (ES) cells. Embryos are the only sources of ES cells. On the other hand, the in vitro reprogramming of adult differentiated cells transforms them into undifferentiated stem cells. The method allows the production of iPS cells ...

The paper with the title “Hemoglobin: A Gas-Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans” has been researched and written by researchers from University of Adelaide, Australia. Some of the important points related to this paper have been presented under the questions.

Introduction and Background:

Hypothesis of the paper

Researchers hypothesized and explored the temporal variations of hemoglobin expression and the genes controlling heme biosynthesis. They have also hypothesized that the possible function of hemoglobin is oxygen transport during in vitro maturation under low oxygen incubation conditions and tested it.

Why authors worked on this experiment?

Initially researchers found Hbb and ...

I am responding to an article dated 16th December, 2013 posted on Articlesbase under the caption health medical tourism written by Sanjaygupta. It is entitled ‘Go Surrogacy India a place to where dreams come true.’ The author’s argument advances that the options offered for surrogacy in Indian are phenomenal because the process is 100% successful; far less expensive than when conducted in United States of America and other developed countries; laws are more parent-friendly; quality of treatment is internationally compatible and surrogates are easier to find (Sanjaygupta, 2013). This is definitely commendable, but the article while discussing the ...

The experiment aimed to show that Blm10 protein elevates the turnover of proteasomal substrate through an active gating mechanism. This was to be done by showing that Blm10 enhances degradation that is proteasome-mediated in vitro using hypophosphorylated unstructured tau-441 as the substrate.

The objective was achieved using various stains such as BY4741, yMS63, yMS94, yMS122b, yMS131, yMS152, yMS159, and yMS476 among others. To start with, the proteasomal activity was assayed by determining gate opening in yeast CP/20S proteasome by the C terminal peptides. Phenotypic analysis was also done by growing the stains overnight in YPD and diluted to a density ...

1. Personal perspective of the future of epidemiological research

Epidemiology is a wide and diverse field that requires a researcher to narrow down to one of the several paths. My perspective of the future epidemiological research has to do with medical research. There are several emerging infectious and noninfectious disease that continue to threaten the very existence of the human race. Of particular interest, the so called lifestyle disease such as cardiovascular disorders, cancer, diabetes, obesity and many others, have an immense impact on the quality of human life due to the fact that many reports indicate that the prevalence of these diseases (once thought to be diseases of old ...

Kathyann

Abstract Dental care substances with stannous fluoride as an active ingredient may be helpful in allowing re-hardening or in increasing the resistance of teeth surfaces to further acidic or mechanical impacts. However, stannous fluorides are applied together with brushing and depending on how and when tooth-brushing occurs. This research focuses on the potential harmful and helpful effects associated with the use of stannous concerning erosive wear. Active ingredients like fluorides or agents that have unique anti-erosive properties were shown to offer protection against erosion and combined erosion and abrasion to some extent. The abrasive effects of dentifrices may ...

Neupogen is a granulocyte colony-stimulating factor that is in man-made form. The bacterium that is used in making this drug is Escherichia coli. Granulocyte colony-stimulating factor substance is produced by stimulation of the body cells. Neupogen drug has been widely used and has shown great effectiveness previously in patients suffering from cancer. It is preferred to people fighting cancer since it can stimulate the replication of the white blood cells in the body mostly referred to as the neutrophils. The benefit of the production of more blood cells among these patients is because the latter reduces their body’s’ vulnerability ...

Background

Early scientists and nutritionists believed that the most vital nutrient in living organisms, for building and maintaining the body was the protein. The role of proteins as enzymes came into existence several years later. This was around 1926 when experiments showed that enzymes were proteins. Insulin was the first protein to be sequenced. Hemoglobin was the first structure of proteins to be resolved. Myoglobin was then resolved later.

X-ray diffraction analysis (X-ray crystallography) led to the determination of the structure of the proteins (three-dimensional structure). The purification of proteins can be made possible from cellular compounds. Several methods have ...

Managing Environmental Issues Simulation

Managing Environmental Issues Simulation Colney and Pitts is a California based pharmaceutical company generating annual sales of $50 million. It is the chief producer in America of herbal remedies for prostate illnesses like prostate cancer, prostatitis, benign prostatic hypertrophy, incontinence, dysuria, polyuria and nycturia. The company has learned that the Pygeum or Prunus Africana can be found in Aberdares mountain ranges in eastern highlands of Kenya. With this information, the company wants to build a manufacturing plant near the mountain ranges. It has been known that the Pygeum or Prunus Africana can be used as an alternative medicine for ...

Introduction

The neurophysiology of nerve pulses in the cell were the subject of the online laboratory experiment in order to better understand neuron communication from inside a cell to the outside of a cell. One of the incredible characteristics of the nervous system is its capability to communicate due to the existence of neurons. Neurons show a measurable response to their environment by producing an electrical signal. We can smell due to olfactory sensory neurons that can directly react to perfume, food or anything with an odor; these odors are the sensory stimuli. The function of the receptor potential is to trigger an ...

INTRODUCTION:

The stem cell research has been debated ever since the birth of it. However, the categorization of the stem cell research has been defined in a wrong manner. Moreover, the media is sensationalizing the topic. This has resulted in a wrong notion that all stem cell research is unethical. This is a wrong notion of the research because there are two types of stem cell research. The adult stem cell research is ethical than the embryonic stem cell research. Adult stem cell research refers to the usage of matured cells from mature adult human being without killing the host. On the ...

Complete Name of Professor

In his book entitled Redesigning Humans: Choosing Our Genes, Changing Our Future, Gregory Stock observes that genetic profiling and pre-conception screenings will soon become obligatory because of its supposed ideal advantages. He believes that humanity will soon be tracking the path to genetic manipulation. Many healthcare facilities in many parts of the world today offer pre-implantation genetic diagnosis, which aims to evaluate the health of embryos whether they are inflicted with diseases or have higher chances of developing genetic disorders. Also, there are medical procedures available today that would allow prospective parents to avail of sex selection. Stock finds ...

There exists about 300,000 plant species on earth, and every individual plant hosts one or more endophytes. However, it is just a few of these plants that have been researched relative to their endophytic biology. Hence, the chance for isolation of new, as well as, interesting endophytic bacteria in plants are important. Endophytic bacteria can be referred to as bacteria that can be isolated from tissues of a surface-disinfected plant or bacteria extracted from inside of a plant, which cause no visible negative effects to the plant. Almost every plant is populated by varied bacteria referred to as endophytes. Majority of ...

Discussion

Periostin, an ECM protein, was named because of its expression in the periodontal ligament and periosteum of adult mice.30 PN is expressed in tissue growth and remodeling, as in cardiac and valvular development and disease, wound repair, tumor tissues, and inflammation.31-37 PN is also widely expressed in various atherosclerotic vascular lesions: it could be located in the intima, preatheroma regions, and the extracellular matrix of advanced lesions with calcification. PN variants are associated with extent of atherosclerosis in young persons, which suggest the importance of PN in the development of atherosclerosis in the arterial wall.38,39 This study used ...

A review of the article by Jankowska, et al., 2008, titled “Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells” Jankowska, et al in their article published in the journal “Molecular Cancer” demonstrate the expression of hCG-beta in cervical cancer and examine the functional significance of hCG- in-vitro cervical cancer cells by making use of modified U1snRNA to silence the mRNA of hCG-. Silencing of the maturation of mRNA by use of a novel silencing technique results in inhibition of growth and apoptosis of cervical cancer cells. Lipofectamine 2000 ...

[First Last Name]

English [Number]

[Date Month Year]

Introduction Originally, 3D printing technologies were designed for nonbiological materials, such as metals, ceramics and thermoplastic polymers (Murphy and Atala 6). However, recent advances have enabled 3D printing of biocompatible materials, cells, and supporting components into complex 3D functional living tissues, giving rise to a new technology referred to as ‘3D Bioprinting.’ However, unlike non-biological 3D printing, it involves important complexities, such as choice of materials, cell types, growth and differentiation factors, and technical challenges related to the sensitivities of living cells and the construction of tissues (Murphy and Atala 1). Overall, 3D ...

Part I

C. Discuss Cherlin’s argument for why Americans have both high rates of marriage and high rates of divorce compared to other industrialized nations. Andrew Cherlin reviewed the historical changes while noting that marriage still remained the most common and effective living arrangement for bringing up of children. However, children are more likely to grow up in single-parent families, and to experience instability of families especially minority and poor children. His view why the US has high marriage and divorce rates, which is called family metabolism, is attributed to cultural and economic forces that have continually transformed family ...

Microbiology is the study of microorganisms. Microorganisms are those organisms that are less than a millimeter in size and require the help of a microscope to view them. Microorganisms can be unicellular or multicellular but have very simple cell structure, unlike the highly developed, complex organisms such as larger animals and humans. Microorganisms are classified based on criteria such as their cell structure, pattern of growth, nutrition, reproduction method, etc. However, microbial classification is a challenging task due to the dual nature of some microorganisms that exhibit both animal and plant like characteristics. Some microorganisms are photosynthetic like plants but they also ...

Lab report #

Introduction In recent times, synthetic antibiotics have caused resistance in microorganisms and also, have shown unwanted side-effects. This has demanded for alternate therapies to combat infections. Scientists have developed interest in plant extracts, especially the ones that are traditionally used as spices, for new antimicrobial agents. Spices are traditionally used to give a distinctive flavor to the food but, they also contain potential antimicrobials which are also documented in literature. Even Louis Pasteur demonstrated the antibacterial effect of garlic and onion extracts. Salsa contains three main ingredients which have potential antimicrobial compounds. First, Lemon (Citrus Limon) is reported to ...

Introduction

Composition of Dietary Fiber Dietary fiber is an essential component in nutrition and health (Mora et al., 2013; Klosterbuer, Roughead, & Slavin, 2011). Thus, the present study will explore the effect of particle size and source of fiber on the level of cholesterol in the human digestion system. Primarily, dietary fiber is a constituent of plant foods and includes materials that have diverse morphological and chemical structure. Such materials resist enzymatic action in the human digestion system (Dhingra et al. 2012). In the gastrointestinal tracts, the dietary fiber forms a matrix that has both amorphous and fibrous characteristics. The matrix’s ...

The product opportunity is based on the consistent on awareness on different types of cancer with a global date set to create global awareness annually. The awareness programs and campaigns are largely aimed at advocating need for testing among patients for early diagnosis in relation to creating a cancer free environment. However, the fact that those diagnosed are offered a new chance to fight the disease indicates the essence of chemotherapy drugs to facilitate full recovery of these patients. The significant problems that Chemotherapeutic Anti-cancer agents are likely to solve due to the compounds that it possess with potent activity within ...

Article Analysis: Ad5 Specific

Article Analysis: Ad5 Specific Since its discovery in 1983, scientists and researchers have been working hard to neutralize the threat of HIV against humans . This advance has included many strategies. Researchers have tried counterattacking the virus while it still hibernates in monkeys, once it was concluded that is where HIV originated . Other strategies have included offsetting the virus after it becomes active in humans. Unfortunately, some of these vaccinations, such as the Ad5 vaccination, allowed the vaccine to progress further, as seen in human trials . Other vectors, such as CMV were used in order to correct Ad5, and ...

Abstract

The primary aim of anticancer therapies is to induce the death of tumor cells. Cisplatin has done a good job in achieving this aim, which explains why it is the most commonly used adjuvant therapy of cancers. However, the success of applying CDDP and any other platinum compounds for cancer chemotherapy depends enormously on the ability to manage its side effects. This paper reviews the studies and literatures on cisplatin and some other similar platinum compounds. It presents its history and background, chemistry, mechanism of activity, as well as the side effects.

Introduction

The cisplatin has been described as a tiny little ...