Essays on Polymerase Chain Reaction

Definition:

Ebola virus, well known for its hemorrhagic effects produced inside as well as outside of body. This virus progresses with bleeding, which leads to organ failure and if not treated properly can also cause death. ("Ebola virus and Marburg virus - Mayo Clinic", 2016). Ebola is also termed as Ebola Hemorrhagic fever because of its hemorrhagic property and it is also known to cause the death of 90% effected people ("Ebola Virus: Symptoms, Treatment, and Prevention", 2016). There are 5 strains of this virus and four of them are known to cause deadly symptoms like hemorrhage. Ebola have its ...

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Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

Introduction

Tooth development involves a complex process whereby teeth are formed from embryonic cells, and then eventually mature after erupting as evident in the mouth (Eckhardt, Jágr, Pataridis, & Mikšík, 2014). The development usually originates from mesoderm and ectoderm. At the ectoderm found in the oral cavity, there is the development of enamel and other tissues also come from associated mesenchyme. All teeth don’t develop at the same time, but rather in stages (Eckhardt, Jágr, Pataridis, & Mikšík, 2014). First buds are always observed in the region of anterior mandible, followed by anterior maxillary area then finally ...

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In archeology, DNA analysis has been used as an investigatory tool since it became available to scientists. DNA analysis, which is a constantly developing and expanding field, has allowed archaeologists to precisely and decisively obtain results that solve archaeological, historical, and evolutionary questions. Unfortunately, the methods for using ancient DNA analysis tend to destroy the samples from which the DNA is extracted. The results can easily be affected by external contamination as well, which requires the research to be conducted within specialized laboratories by trained laboratory personnel (Arandjelovic, Guschanski, Schubert, Harris, Thalmann, Siedel, & Vigilant, 2009). DNA analysis has been ...

Improving The Efficiency of Cytomegalovirus (CMV)

Abstract The Cytomegalovirus (CMV) is known to be an important pathogen among patients with prolonged confinement to the intensive care unit and longer hospital stays (Vincent, 2010). The virus is recognized to be a common form of the herpes virus, which the Centers for Disease Control described to be present in about 50% to 80% of the people in the United States who are in their 40’s (Mandal, 2014). The infection can actually affect children and adults alike, however the virus has the greatest impact among certain individuals, especially among children and those with a compromised immune system and ...

Abstract

A polymerase chain reaction also known as PCR refers to a technique that is used in the amplification of a DNA fragment making huge numbers of the original fragment. The process uses an enzyme known as DNA polymerase to multiply the DNA fragment. Other components that are necessary for a PCR reaction are the DNA template that is provided by the sample being multiplied, the primers, and nucleotides. The experiment aimed to examine a number of human DNA polymorphisms, the PV92 Alu polymorphism and the mitochondrial control region using human DNA. This was achieved by isolating the mitochondrial DNA from the mouth cells ...

Question 1

The type of bloodstain evidence at the scene is a cast off pattern. According to Becker (2005), a cast-off pattern normally results in blood striking nearby surfaces such as walls, floors and ceilings. A cast-off pattern indicates that a blunt force trauma to the head. The large wound on top of the head is indicative of repeated use of the blunt force weapon on the victim. The first step in the documentation process of the room would involve conducting an initial scan of the room to ascertain the evidence present. This would then be followed by photographing of the scene in ...

1. In your own words explain why this paper is important or significant The article is very informative and significant since it introduces another breakthrough in DNA sequencing and analysis. It highlights the polymerase chain reaction which is the new way of identifying mutant genes in both homozygous and heterozygous organisms. The technique uses primers to amplify segments of the DNA by many folds such that it clearly identifies the mutant genes faster than all the other methods. The paper points out important milestones that genomics and gene sequencing has undergone over the years. Geneticists usually use the ...

Abstract

This research paper will discuss the significant contribution of DNA fingerprinting in the criminal justice system for being able to provide a reliable basis for conviction. DNA fingerprint analysis is referred as an accurate indicator to determine the guilt and innocence of an accused. The history, importance and various DNA fingerprinting techniques shall be discussed. In addition, DNA fingerprinting is considered as more superior than other forensic science methods since the technology has improved over time. DNA fingerprinting has allowed the test of smaller amounts of material, faster testing procedures and more conclusive results. Finger ridge analysis is science and ...

Polymerase Chain Reaction

Polymerase chain reaction known as PCR was developed by Kary Mullis in 1983. It is a Molecular Diagnostic technology used for amplification of DNA. A single copy of DNA can be amplified to higher magnitudes with a thousand copies of a single DNA sequence. PCR has now been developed as a Molecular Diagnostic tool for Cancer and mainly to detect the early onset of leukemia or lymphomas. It is widely used as a diagnostic tool in the field of microbiology and is also utilized in detection of infectious micro-organisms y detecting specific disease causing genes. In the year 1993, Mulis was awarded the Noble ...

The advancement of technology has precipitated a revolution in scientific understanding over the last several decades. Advancements in research tools has led to new insights into every facet of scientific discovery within the realms of chemistry, physics, and biology and all the sub-disciplines forming their constitution. Increased discovery has brought with it opportunities for cross discipline cooperation which has resulted in the formation of new disciplines and has galvanized scientists to press the boundaries of research and application as has never been seen in human history. One such field testing those limits is genetics. Today’s geneticists have expanded beyond ...

Introduction

This report is completed after a survey of cardiovascular and human papillomavirus disease that are found among the united states women.Reference The main source of this report is from the national health and nutrition examination survey that was conducted from 2003 to 2006.Kind of research The research was experimental as sample of participants had to be collected and then examined. Some test was conducted in order to now the real result of the expected research. From the data and result that were collected, a lot of analysis was done and conclusions reached at in a decisive manner. Purpose This ...

Introduction

The emergence of real-time polymerase chain reaction (PCR) assays was much of a relief in the medical field especially given the fact that this model offers the most accurate way in the process of determining false-negative outcomes (Burggraf & Olgemoller, 2004).This ideology also finds support in the works of Nolte (2004) who asserts that, following the discovery of PCR and its application in a clinical setting, it has become possible to use nucleic acid amplification methods as transitions from research to clinical laboratories. This makes it possible to have a wide array of application in addressing issues relating to genetics, pharmacology, oncology ...

Abstract

A-kinase anchoring proteins (AKAPs) are a group of structurally varied scaffold proteins which are found to regulate the PKA pathway by localizing the protein kinase A and some specific enzymes at sub cellular level. So far several of the AKAPs have been found to play critical role in the regulation of phosphorylation of several PKA dependent substrates which are necessary for the modulating the cardiac function. Abnormalities in these AKAPs genes have been found to cause various forms of cardiac disease. The most prominent AKAPs are the AKAP9 and AKAP13. Mutations in the AKAP9 gene has been found to be ...

Introduction

The most widely used model when investigating the breast cancer is T47D cell lines. These cell lines are easily spread, easily manipulated genetically and when used in the experiment, it provides good results. They have been used to examine new therapies and the and the breast cancer pathobiology1. Over many years HER2 has 25% - 30% of human breast cancers have been caused by gene amplification. The monoclonal HER2 and the EGFR are the two HER2-targeted therapies that are used for approval in the most clinics. During growth, HER2 is used to regulate the cell growth and assist ...

Sometimes Forensic Casework items consist of less than 100 picograms (pg) of template DNA which, if we assume 3.5 pg of DNA per haploid cell, can be equated to approximately thirty haploid cells or fifteen diploid cells are termed as LCN DNA (low template DNA or LT DNA). (Gill 17) Such low copy number (LCN) samples exist due to several factors like damaged or degraded DNA, oligozoospermia or aspermic perpetrators or extended interval post coital samples, where there is loss of sperm over time because of the effects of drainage or host cell metabolism. From crime scenes, Trace ...

Methodology

The first step involved ligation of digested polymerase chain reaction (PCR) products into pGLUX1 proteins. Thereafter, the resultant proteins were transferred onto nitrocellulose filter membrane (NCF). This is where the ligation reaction was used to transform E. coli XL10-gold cells. Thereafter, specific cells were selected to undertake DNA plasmid isolation through being subjected to 200 volts for 50 minutes. In confirming complete ligation of the cells, restriction digest using XhoI and KpnI enzymes were used. The digested products were then subjected to agarose gel electrophoresis. In this case, the transfected culture was grown at 37°C under drug selection ...

Fungal endophthalmitis (FE) is endophthalmitis caused by any of a wide variety of fungal pathogens. Generally, endophthalmitis is an inflammation of the anterior chamber and vitreous (the substance that composes most of the posterior chamber) of the eye and can spread to the retina (the layer of the eye contiguous to the vitreous) and the choroid (the layer behind the retina) (Wu).

Causes

In FE, the fungal infection may be either endogenous, i.e., spread from some other site of fungal infection in the body) or, more commonly, exogenous (acquired directly from outside the body, such as by trauma or at the ...

Abstract

The main Babesiosis diagnostic tests available are microbiological and serological. Unfortunately these tests have low specificity and sensitivity. Two molecular tests, the Polymerase chain reaction (PCR) and Fluorescent In-Situ Hybridization (FISH) tests, developed by IGeneX have great potential. Using 100 mice models the sensitivity and the specificity of the two tests will be determined and compared with the serological test.

1.0 Introduction

Babesiosis is a disease caused by an intracellular parasite, Babesia, which infects erythrocytes. Four main species of this parasite are known to infect humans; Babesia microti, B-MOI, B. divergens and B.duncani. Babesiosis is transmitted by ticks and has symptoms such ...