Essays on Proteasome

Vid30 complex participation in the degradation of CDC25 in yeast and their role in Ras/ cAMP/ PKA pathway

Background: Unicellular organisms such as Saccharomyces cerevisiae in its total life cycle is exposed to diverse and changing nutrient conditions. Thus, in order to survive and achieve a competitive advantage, it has adapted itself such that it not only uses the wide range of available nutrient sources but also the sources that are nutrient rich. It mainly uses fructose and glucose by rapidly fermenting to alcohol. In response to these nutrient conditions, the intracellular trafficking transcription and turnover of protein from nutrient transporters (e.g. Hxts) get regulated. Therefore, when the availability of the glucose increases, the expression of low- ...

Abstract

This paper is about the investigation of Parkinson’s disease, and in particular is involved with the examination of its symptoms, stages, causes, prevalence, diagnosis and treatment, and social impacts. Finally, the paper analyzes and evaluates two ongoing on Parkinson’s disease researches, which are also examined in respect to their impact on Parkinson’s disease patients. Key words: Parkinson disease, Parkinson disease causes and impacts, Parkinson disease symptoms and prevalence, Parkinson disease diagnosis, and Parkinson disease ongoing research.

Idiopathic Parkinson disease (PD) is a progressively evolving disease with main manifestations resting tremor, muscle stiffness, slow movement and loss ...

The title of the paper that is reviewed is: “Sumoylation and Phosphorylation of α-synuclein”.

It is a research article that explores the molecular mechanism underlying αSyn toxicity. αSyn is a protein that is seen in the inclusion bodies of dopaminergic neurons in patients with Parkinson’s disease. Parkinson is an important neurodegenerative disease, and the cure of the disease is still unknown. In-depth understanding of the molecular pathogenic of the disease will help in determining suitable cure. Through the insight obtained from a literature review and previous studies, the authors identified that post translational modification of αSyn, could affect its physical characters like the ability to form aggregates, solubility in cytoplasm and its ...

The experiment aimed to show that Blm10 protein elevates the turnover of proteasomal substrate through an active gating mechanism. This was to be done by showing that Blm10 enhances degradation that is proteasome-mediated in vitro using hypophosphorylated unstructured tau-441 as the substrate.

The objective was achieved using various stains such as BY4741, yMS63, yMS94, yMS122b, yMS131, yMS152, yMS159, and yMS476 among others. To start with, the proteasomal activity was assayed by determining gate opening in yeast CP/20S proteasome by the C terminal peptides. Phenotypic analysis was also done by growing the stains overnight in YPD and diluted to a density ...