Get exemplary FREE essays about Apoptosis to encourage your personal muse

- Cell Culture and Cell Lines:
Three breast cancer cell lines: CAL120, EVSAT and MDA-MB-231, were cultured individually, in RRMI 1640 supplemented with 20% Fetal Bovine Serum (FBS), 100U/ml Penicillin, 100U/ml Streptomycin and L-Glutamine. The cells were cultured and maintained in an incubator at 370C supplied with 5% CO2. For subsequent experiments, the cells were further cultured till confluence, in 6 well and 96 well plates using RPMI+20%FBS.
1.2 Treatment of Cells:
For treatment, 1x106 cells were seeded in 6 well culture plates, and incubated for 24 hrs. After 24 hours, the old media was replaced with

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What is the evidence that convinces you Cancer Stem Cells are a subpopulation of tumour cells with distinct tumour‐initiating capacity?
What are cancer stem cells (CSCs)? They are cancer cells that have similar characteristics as normal stem cells. CSCs have the potential to give rise to other cells types, and thus are tumorigenic in nature. These cells can generate tumors via stem cell processes of self-renewal, as well as differentiation into different cells (Wang, Chiou & Wu, 2013). CSCs have been found to persist in tumors in the form of a district population and often cause relapse and

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Regulation of Xiap Protein in Breast Cancer
Breast Cancer
Breast cancer is the type of cancer that develops in the tissues of the breast. Two most commonly reported types of breast cancer are “ductal carcinoma” and “lobular carcinoma”. Ductal carcinoma is initiated in the lining of the milk ducts, which are thin tubes involved in carrying of milk from lobules of the breast to the nipple. Ductal carcinoma can be in the form of Invasive ductal carcinoma (IDC) as well as Ductal carcinoma in situ (DCIS). IDC is the most commonly found type of breast cancer with nearly

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DNA DAMAGE

1. Introduction
The p53 gene is a tumor suppressor gene that is activated whenever a cell is subjected to cell damage. The p53 gene determines whether a cell should survive a damage or should undergo apoptosis. These decisions are regulated by posttranslational modifications. Phosphorylation of the serine 46 residue is one such modification that is relevant to this study. However, it is unclear why a Ser46 phosphorylated p53 favors apoptosis over survival (Feng et al. 2006, p. 2812; Taira et al. 2014, p. 717).
Studies have shown that the elusive molecular mechanism of Ser46 phosphorylation of p53 induce apoptosis

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The title and abstract suggest that the paper is an attempt to show that in the cartilage growth plate, abnormal chondrocyte apoptosis is influenced by genetic background as well as deletion of CHOP. The research is interesting because it enables the discovery of whether whether the abnormal chondrocyte apoptosis that is seen in the mutant mice growth plate is CHOP-mediated. The research builds on the research done by Delot, King, Briggs, Wilcox & Cohn (1999) and Hecht et al. (2001), who demonstrate that type 3 recurring mutations are responsible for most of the Pseudoachondroplasia (PSACH) cases, which are believed to

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Introduction

Antigen Presentation
Antigen presentation plays a pivotal role in the immune system through which pathogenic antigens are processed and coupled along with major histocompatibility complex (MHC), and presented to T cells for their effector response. Macrophages, B cells and dendritic cells are the primary antigen presenting cells (APC’s). Intracellular antigens generated by viruses, or some cytoplasmic bacterial antigens are digested by the host cells and coupled with MHC Class I by Transporter associated with antigen Processing complex (TAP), which transports them to the cell surface and presents to CD8+ cytotoxic T cells (Germain, R 1994). These cells elicit

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