Essays on Globin

Introduction

Sickle Cell Anemia or Sickle Cell Disease is an inherited blood ailment. Those suffering from Sickle Cell Disease have abnormal S/ sickle hemoglobin in the red blood cells. Hemoglobin is the protein in the blood that aids in carrying oxygen throughout the body. The ailment takes its appellation from the shape of the red blood cells of a person affected by Sickle Cell Disease, which have the shape of a sickle. They are stiff and adhesive rather than a disk like normal cells. The misshapen cells tend to get trapped in the small blood vessels hindering the movement of ...

Abstract

In the last century, the knowledge of molecular biology has increased, opening new opportunities for addressing different diseases that have been affecting all populations. Before, genome and proteomic studies were seen as complicated field due to inability to get significance understanding of disease components at the molecular level. However, with the expansion of molecular biology, different techniques and tools have been developed to aid diagnosis and treatment of diseases. In this study, these tools have been investigated to understand how the knowledge on molecular diagnosis helps in detection and treatment of diseases. This study focused on the application of ...

Introduction

Blood consists of erythrocytes and leucocytes suspended in it. Blood is pumped from the heart to the body through the arteries and it enters back in the heart through the veins. Blood without cells is known as plasma while serum is the straw colored liquid obtained after the removal of the clot. Plasma contains different proteins, nutrients and waste materials. There are different cases of blood disorders observed in humans.

Blood cells and platelets

The most abundant cells in the blood are the anucleate, biconcave erythrocytes. They contain a red pigment known as hemoglobin (Hb) that carries dioxygen (O2). Hb contains four subunits each ...

Beta thalassemia is an inherited blood disorder triggered by the absence or reduced production of hemoglobin. It is regarded as a genetic or hereditary insufficiency in the permutation of beta globin chains (Galanello and Origa). A newly born child needs two globin genes from each of the parents to generate beta globin chains. If one or two of these genetic factors or materials are deficient, it will form into the beta thalassemia disease. Moreover, beta thalassemia also hinges on how many genes are transmuted. Beta thalassemia minor is produced if only one of the globin genes is mutated. If both ...

Objective 2

Introduction 2 General background 3 Resistance mutants 4 Hemoglobin mutants 4 Sickle Cell Anemia and Genetics: Background Information 5 Connection of Sickle cell with Malaria 6 Method: 7 Introduction of Recombinant DNA technology in diagnosing disease: 8 Discussion (implementation of recombinant DNA technology to solve the issue) 9 Conclusions 10

References 11

Objective This report targets to study a group of people that has developed resistance to malaria. It will focus on the use of recombinant DNA technology to study this cohort and gain insights into the nature of this observation. This ...

Hemoglobinopathies: Analytic Diagnostic Techniques

Hemoglobinopathies and Thalassemia’s are a kind of Genetic disorders caused mainly by a mutation or deletion of Globin genes in Hemoglobin. Hemoglobin consist of α or β Amino acid chains. An alteration in α chain production leads to α Thalassemia and alteration in β chain causes β Thalassemia. Hemoglobinopathies on the other hand may be caused due to the structural abnormalities or alteration of amino acid sequences in the Globin genes. These are mainly hereditary and constitute to around 4 to 5% of the world population. (WHO Statistics, 2012) Each year around 250,000 to 300,000 new born children have either hemoglobinopathies or thalassemia. Both ...

Introduction

An article published by the Cochrane Collaboration and posted in the Cochrane Library in 2012 caught my attention. The article stated that no quasi-randomized or randomized clinical trials of gene therapy for sickle cell disease have been documented. The article further stated that no conclusions and recommendations are attributed to the application of gene therapy on sickle cell disease (Olowoyeye & Okwundu, 2013). This statement inspired me to evaluate the current developments in gene therapy that targets sickle cell disease. Sickle cell disease is one of the oldest genetic disorders and yet few attempts have been made to develop gene ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

Epidemiology of Thalassemia among the population of Malta

This discussion is about epidemiology of thalassemia in Malta. Thalassemia is the given name of a category of genetic blood confusions typify by anemia because of augmentation of red blood cell destruction (Barnes, Plotnikoff, Fox, and Pendleton, 2000). Hemoglobin, it is the conducting of oxygen of the red blood cells dwelling by the two diverse proteins which are the alpha and beta. In case that the body doesn’t construct sufficient with these two proteins, the red blood cells is inadequate and cannot bring enough oxygen (Sembulingum K. Et Al, N.D.). It is a severe with a more than few of health ...

The cDNA that was used in the experiment is the transfer tRNA. The coding region refers to the gene portion (DNA or RNA) that has axons in it and they code proteins. It’s also known as the coding sequence or the coding DNA sequence. The coding region sequence is surrounded near the 5th end of the start codon and the 3rd end with a stop codon. Therefore, the total addition of the genome of a living thing consists of the gene coding regions. The identification of the cDNA coding region sequence is not straight forward because the existing cells only ...

Recent studies have shown that mouse and human fibroblasts can be re-encoded into an advanced stem cell–like state when a combination of transcription aspects is introduced. Conversely, the salutary probable of induced pluripotent stem cells have remained indefinite. One of the things that make this article interesting is that it explores how the mice can be salvaged after uprooting by use of cultivated sickle cell anemia mouse model. This article also exclusively stipulates how this was achieved after rectification of the human, sickle hemoglobin. The article also presents the major goal of human therapy, which is developed, using methods ...

The differences between the oxygenated and deoxygenated states of hemoglobin

a. Red Blood Cell with oxygenated hemoglobin (diamagnetic) has no effect on the magnetic field b. Deoxygenated Red Blood Cell with deoxygenated hemoglobin which is paramagnetic affects magnetic field Erythrocytes of the blood act as carriers of hemoglobin; a four unit protein that through binding process with oxygen molecules, thereby forming oxyhemoglobin, transports oxygen to various part of an animal’s body. Oxygenated and deoxygenated states of hemoglobin have different quaternary structure. The disparity in quaternary structures of these two kinds of hemoglobin is attributed to the binding of oxygen with hemoglobin. The beta chains in oxygenated hemoglobin ...

The differences between the oxygenated and deoxygenated states of hemoglobin

Erythrocytes of the blood act as carriers of hemoglobin; a four unit protein that through binding process with oxygen molecules, thereby forming oxyhemoglobin, transports oxygen to various part of an animal’s body. Oxygenated and deoxygenated states of hemoglobin have different quaternary structure. The disparity in quaternary structures of these two kinds of hemoglobin is attributed to the binding of oxygen with hemoglobin. The beta chains in oxygenated hemoglobin are much closer to each other compared to the ones in the deoxygenated hemoglobin (Campbell &Farrell, 2009). In the same light, the quaternary structure is affected by the hemoglobin’s ...