Abstract Chromatographic techniques are used to separate different components in a mixture based on their solubility in the mobile phase and adsorption on the stationary phase. The most soluble components move further with the mobile the components that adsorb more on the stationary phase move a shorter distance. The main component of green leaves is chlorophyll. However more information can be obtained on the content of leaf extracts by separating a homogenate made from a leaf sample. In this experimental report, spinach leaves were used to obtain a homogenate that was separated on paper chromatography to obtain the various leaf pigments. Isopropanol was ...
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