Essays on Mrna

Gene regulation involves the cellular process that aims to prevent and control the manner in which gene expression occurs. Gene regulation includes a range of mechanisms used by the cells to decrease or increase the production of specific products of the genes. Many sophisticated programs of gene expression are observed in biology that includes response to the environmental stimuli and triggering of other developmental pathways. Steps involving expression of genes can be controlled from the initiation of transcription, to processing the DNA, and to the mechanisms involved in the post translational modification of the proteins. Gene regulation is important for ...

The process of DNA replication at biochemical level

DNA or deoxyribonucleic acid is a biopolymer that encodes all the structures needed for the organism’s lifespan and development. In a cell it is located in the nucleus, mitochondria and chloroplasts. DNA consists of four types of deoxyribonucleotides, the sequence of which is used to store the data.

The essential characteristic of DNA molecule that allows it to pass the stored information to the next generations of cells or organisms is the way of self-reproduction known as replication. The molecule consists of two complementary chains that are held together with the help of hydrogen bonds between the complementary bases. ...

DNA (Deoxyribonucleic Acid)

Deoxyribonucleic acid, DNA, is the primary structure that carries the genetic material in cells of living organisms and some viruses. The DNA is made up of strands of nucleotides that are made up of nitrogenous bases, a phosphate compound and, a ribose sugar. The nucleotides form a ring or ladder that over lapses to form double helix strands of polynucleotides. DNA aggregates to form the chromosomes that are strictly found within the nucleus of the cell. Some DNA are found in the ribosomes outside the nucleus. In the DNA structure, the series of the bases is arranged, and the sequence in one ...

Gene expression refers to the mechanism by which information from a gene directs the synthesis of proteins. This process is utilized by eukaryotes and prokaryotes. Gene expression involves various steps. These includes; transcription and translation. The central dogma indicates the flow of genetic information from the Deoxyribonucleic Acid to Ribonucleic Acid and synthesis of the required protein.

Transcription is the production Ribonucleic Acid copies of Deoxyribonucleic acids in the nucleus. The DNA consists of two anti parallel complementary strands, each having 5’ and 3’ ends. The strands serve as a template strand for the production of an RNA transcript. The ...

The paper with the title “Hemoglobin: A Gas-Transport Molecule That Is Hormonally Regulated in the Ovarian Follicle in Mice and Humans” has been researched and written by researchers from University of Adelaide, Australia. Some of the important points related to this paper have been presented under the questions.

Introduction and Background:

Hypothesis of the paper

Researchers hypothesized and explored the temporal variations of hemoglobin expression and the genes controlling heme biosynthesis. They have also hypothesized that the possible function of hemoglobin is oxygen transport during in vitro maturation under low oxygen incubation conditions and tested it.

Why authors worked on this experiment?

Initially researchers found Hbb and ...

Deoxyribonucleic acid, DNA, is involved in the manufacturing process of all proteins in the body. It synthesizes proteins that repair and replace worn out tissues in the body. Stem cells (undifferentiated cells with the ability to differentiate) researches have shown that cells from some regions of the body can differentiate into different tissues. Therefore, they can replace worn out tissues and hence function as a repair mechanism. These cells can be induced to become tissue or organ cells. Scientists argue that perhaps the stem cells may carry out similar functions as DNA hence can replace it. This paper seeks to ...

Akash, K., Jay, S., & Nelson, P. (2011). Mutations Genome Interrupted: Sequencing of Prostate Cancer Reveals the Importance of Chromosomal Rearrangements. Genome Med , 3-23.

Prostate cancer is a huge public health menace but despite it being the most popular diagnosed cancer, very little is known about its cause due to the heterogeneity in the cells of the prostrate and also lack of an organized on the genes expressing in this tissue. In this article, Akash, Jay and peter try to show sequencing of prostate cancer reveals the importance of chromosomal rearrangements thus paving a way into a discovery of a remedy of the ...

Keywords : Gene therapy, Sickle Cell Anemia, Stem cells, lentivirus, β-globin

Project type: GBE-492 Genetic Engineering Project I. INTRODUCTION, BACKGROUND and AIM Sickle cell anemia is a devastating genetic disease which causes premature death. Sickle cell disease (SCD) is caused due to single amino acid change (glutamine to valine at position 6) in β chain of hemoglobin molecule, which causes the hemoglobin molecules to aggregate. These aggregated hemoglobin molecules make the red blood cells (RBCs) stiff and deformed (sickle shaped). These deformed cells block the normal blood flow. Although this disease is well-characterized, but no long-term treatment 1 is still available for this disease. The discovery of the sickle ...

1. When placed on an agar slab and incubated at 30°C for 2 days, a single yeast cell proliferates into a colony with a diameter of 2 mm containing 108 yeast cells. If the cell has a functional Ade2 gene (Ade2+), the colony will be white; if Ade2 is non-functional (Ade2–), the colony will be red. You take a strain with a mutant Ade2– at the regular chromosomal position (red colonies) and insert a second, functional Ade2+ gene near the telomere. You find that the colonies are a mixture of red and white patches or sectors. Figure 1 shows some colonies; the black regions represent the ...

Background

Early scientists and nutritionists believed that the most vital nutrient in living organisms, for building and maintaining the body was the protein. The role of proteins as enzymes came into existence several years later. This was around 1926 when experiments showed that enzymes were proteins. Insulin was the first protein to be sequenced. Hemoglobin was the first structure of proteins to be resolved. Myoglobin was then resolved later.

X-ray diffraction analysis (X-ray crystallography) led to the determination of the structure of the proteins (three-dimensional structure). The purification of proteins can be made possible from cellular compounds. Several methods have ...

Abstract

Proteins are made up of amino acids and have major structural as well as functional roles in the body. Amino acid chains are synthesized from DNA sequences through transcription and translation. Proteins become fully functional only after co-translational or post-translational modification of the amino acid side chains and folding into appropriate three-dimensional conformations. Even slight changes in the DNA code could drastically alter the protein folding, alter its functioning, and eventually lead to certain diseases. Inherent cellular repair mechanisms, protein degradation pathways and molecular facilitators such as chaperones try to keep proteins in their native, stable conformation by acting ...

Protein synthesis is the process through which the biological cells produce new protein molecules. The process helps in balancing the cellular proteins that are lost through export or degradation. The synthesis of proteins occurs through a series of processes known as translation where the ribosomes are the organelles that are involved in the creation of proteins. Translation is a part of gene expression using the messenger RNA or mRNA that is produced through transcription of the DNA molecule. The produced mRNA is then decoded to produce a chain of specific amino acids using the ribosome complex. The amino acid chain produced is ...

Lab 1: Ethical Issue in the Movie Gattaca. The epicentre of ethical issues in the movie Gattaca is the manner in which an individual ought to be judged. Should it be by the genetic composition one is born with or by the character traits, personality attributes and ambitions chosen by an individual? Vincent is born with genetic weaknesses that could result in ailments in the future. His genetic composition denies him access to his dream job despite the fact that he achieves the required grades in school and is a strong and athletic young man. As a result, Vincent designs a plan to ...

A review of the article by Jankowska, et al., 2008, titled “Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells” Jankowska, et al in their article published in the journal “Molecular Cancer” demonstrate the expression of hCG-beta in cervical cancer and examine the functional significance of hCG- in-vitro cervical cancer cells by making use of modified U1snRNA to silence the mRNA of hCG-. Silencing of the maturation of mRNA by use of a novel silencing technique results in inhibition of growth and apoptosis of cervical cancer cells. Lipofectamine 2000 ...

Human Immunodeficiency Virus, a main cause of worldwide AIDs, belongs to the Lentivirus genus of Retroviridae family. Pathogen causes chronic type of symptoms, taking a long time of latency it constantly keeps replicating inside human body. HIV is categorised into two types, HIV-1 and HIV-2. The genome of this retrovirus consists of two single-stranded RNA molecules. Though, both are involved in AIDS, HIV-2 seemed less virulent and demonstrated more frequent connections with the disease of central nervous system (Fanales-Belasio et al. 5). Like other retroviruses, the genes located on HIV chromosome are gag, pol, env. The functions of gag, pol ...

- Describe the role of the following in protein synthesis - DNA, nucleotides, RNA, ribosomes, rough ER, and Golgi apparatus. DNA or deoxyribonucleic acid is a double stranded hereditary molecule containing the genetic code from which specific protein molecules are synthesized. First step in protein synthesis is synthesis of single stranded complementary RNA. RNA also contains all the components of DNA except a ribose sugar. Special RNA called messenger RNA (mRNA) carries the message to ribosomes. Under mRNA’s direction, transfer RNA (tRNA) delivers amino acids to ribosomes and ribosomal RNA (rRNA) attaches each amino acid to form the final ...

Investigating the Functions of Proteins

The steps made so far in the process of gene mapping has opened up ways in which more studies are done on the human DNA. ENCODE and Genome researchers have so far sequenced more than 90 genomes. With the understanding of genes and their loci, it has been easier to locate and carry out studies on a single gene. Most genes code for various proteins in the body of an organism; it has become paramount that the roles of these genes be identified and prescribed out. The knowledge of the functions of each gene, or the protein it codes for, ...

Abstract:

DNA (Deoxyribonucleic acid) is hereditary material found in most organisms and carries the genetic information required to build and maintain a living organism. DNA was first discovered in the late 1800's, but it was not until scientists unraveled its structure in the 1950s that its importance in genetics and the whole field of biological sciences became realized. This paper assesses the structure, history and applications of DNA into solving real world problems in medicine, agriculture, biochemical research and forensics. DNA can replicate itself which ensures that genetic information and consequently biological traits can be passed on from one generation to ...

Reason for doing the research

The goal of the paper was to investigate whether pharmacological treatment with the pan- Peroxisome Proliferator-Activated Receptor (PPAR) agonist bezafibrate would correct a deficiency of PPAR--coactivator 1α (PGC-1 α) and apply beneficial effects in a transgenic mouse having Huntington’s disease (HD). A number of mice with a deficiency of PGC-1 α were fed on a special diet comprising of bezafibrate and another group was left untreated. The mice were examined for a long period and then euthanized with CO2 inhalation. They died after some time and were dissected with several internal parts (brain, muscles, liver etc) being examined.

Hypothesis

The hypotheses was based on the evidence ...

Abstract

Bones are formed through a process known as osteogenesis from three different lineages. This process takes place through two different methods, which are intramembranous ossification and endochondral ossification. A large increment in bone mass takes place in childhood, as well as puberty, through the formation of endochondral bone. At this point, growth hormones are known to play a role. This paper will focus on the process of cartilage and bone formation and the stimulation of bone formation by the growth hormone. There are a number of factors that regulate the formation of bone, while growth hormone affects a number of factors related to ...

Research Plan

Introduction Toxin-antitoxin (TA) systems incorporate pervasively in prokaryotic genomes that have also been suggested to perform a role in numerous critical cellular operations (Van, & Bast, 2009). These systems characteristically comprise of a two-gene operon encoding a toxic protein molecule which aims at an important cellular purpose and an antitoxin that connects to and hinders the toxin. Control of toxin action is attained via differential constancy of the stable toxin as well as the unstable antitoxin (Kamada & Hanaoka, 2005). In several occasions, the antitoxin also operates as a transcriptional autorepressor of the operon, whereby the degradation of the antitoxin leads to transcriptional introduction ...

Most chemotherapy drugs are designed to block DNA replication; majority of the generally and successfully drugs are DNA-damaging agents. The DNA targeting has proven to result in comparatively potent and discerning distribution of cancerous cells. Compounds which are empirically identified with anticancer activity have been indicated to target DNA as the crow flies or through enzyme inhibition which controls the integrity of the DNA of offers the DNA building blocks. With the revelation of the DNA structure various therapeutic modlaities have been established namely; the antimetabolites which, depletes the nucleotides comprising folic acid, an example being methotrexate. Alkylation agents ...

Chapter 2 – Literature Review

Chapter II The Review of the Related Literature Introduction During the past decades, breakthroughs in the study of Vitamin D and findings pointing to its importance in maintaining health have been made. Apart from the found importance of Vitamin D in human skeletal development, further evidence is attributing the benefits of adequate Vitamin D to the development of other tissues in the body. In addition, the prior belief regarding the amount of Vitamin D needed in the body was changed encompassing new findings that the human body needs more Vitamin D than usually thought (Holick, 2004). ...

Introduction:

The function of many living organisms is coupled with a vast array of cells .These units in turn serve as role playing agents for the fundamental physiology that determines the well being of organisms. However, variations in cellular environment are thought to regulate cell growth, division and survival. A network of molecular pathways or signaling events are interlinked with the cellular functions .Alterations in this specific functional aspect could lead to unnecessary complications which may lead to health abnormalities. In such context , the present description is concerned with highlighting about mTOR Signaling in Carcinogenesis. mTOR stands for mammalian target ...

Abstract

IFITM3, a protein induced by interferon, restricts reovirus infection in host cells and inhibits the viral replication. It affects the proper penetration of the virus by altering the dynamics of the viral uncoating in the endosomes. This study is the first in characterizing the anti-viral activity of IFITM3 on non enveloped viruses that depend on endosome for cellular entry. Following contagion with a virus, the body triggers the release of a class of cytokines, interferons that are involved in first line of defense against infection. Type I interferons are specifically induced in the cells following insult with both DNA and RNA ...

a) Background and Significance Type 2 Diabetes Mellitus is a chronic disorder characterized by two main features: high blood glucose level (hyperglycemia) and insulin resistance. The disorder was formerly referred to as non-insulin-dependent diabetes mellitus. The pathophysiology of type 2diabetes is progressive, characterized by decreased insulin sensitivity, deteriorating cell function, and decreased incretion function (Phielix and Mensink, 2008; Misra, 2008). In human studies, people with type 2 diabetes have a decreased PGC-1α expression compared to healthy subjects (Wagner et al., 2008; Cantó and Auwerx, 2009; Kang, 2012). The skeletal muscles of people with type 2 diabetes and those of their ...

Introduction:

Living organisms are equipped with robust machinery for executing a myriad of tasks to meet the day to day biological challenges. This machinery in turn relies on one or more interconnected pathways or networks composed of nucleic acid, proteins and other essential constituents. To say, it is the precise function of these components which determines the physiological well being organisms. Mammalian target of rapamycin (mTOR) kinases is one such known component that performs diverse physiological roles through its associations with the molecular machinery. mTOR is a protein that responds to metabolism and ageing, intercellular stress to growth regulation, growth factors ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

- Heterogeneous nuclear ribonucleoproteins is a protein that comprises of RNA binding proteins. It is a complex made up of RNA & proteins that is characterized by binding of premRNA. This occurs in the nucleus during the transcription and post transcriptional stages of preMRNA. The protein has different roles which include; prevent formation of secondary structures by preventing folding of pre-mRNA, inhibit the interaction with other proteins, usually has a link with the splicing apparatus and transportation of the mRNA away from the nucleus. This type of protein is a multidomain protein with multiple RNA binding proteins. RNA recognition Motif which is the commonest ...

Genetic testing also known as DNA based test, involves direct examination of DNA molecules to test for genetic disorders and diseases (Gene testing, 2010). According to the Human Genome Project Information (2010), Genetic testing can be used for various reasons to include: - Identifying people who carry one copy of genes causing diseases such as Huntington’s disease. - Pre-symptomatic testing of adult onset diseases/disorders such as Huntington’s disease - Forensic testing and determining paternity. - Newborn screening and pre- natal testing. DNA stands for Deoxyribonucleic acid, and these are chemical Data bases ...

- Describe the phases of meiosis II Meiosis II has 4 phases, prophase, metaphase, anaphase, and telophase. At the end of meiosis I, microtubules move each member of the centriole pair to an opposite spindle pole in both daughter cells. During prophase II, the microtubules attach to the chromosomes causing them to recoil and shorten. During metaphase II, the microtubules, duplicated chromosomes, and motor proteins, interact in a manner that positions all the duplicated chromosomes halfway between the 2 spindle poles. During anaphase II, the connection between the 2 sister chromatids of every chromosome breaks and each of the ...

The cDNA that was used in the experiment is the transfer tRNA. The coding region refers to the gene portion (DNA or RNA) that has axons in it and they code proteins. It’s also known as the coding sequence or the coding DNA sequence. The coding region sequence is surrounded near the 5th end of the start codon and the 3rd end with a stop codon. Therefore, the total addition of the genome of a living thing consists of the gene coding regions. The identification of the cDNA coding region sequence is not straight forward because the existing cells only ...

Introduction: Glucagon-like peptide-1 (GLP-1) based therapy is a novel treatment for type 2 Diabetes. GLP-1 receptor agonists and inhibitors of dipeptidyl peptidase IV improve glycemic control. GLP-1 exerts multiple effects including modulation of a-cell proliferation, apoptosis and exogenesis. Inhibition of gastric emptying and food Intake with stimulation of glucose uptake by adipocytes has also Been reported.

Beneficial effects of GLP-1 on myocardial and endothelial functions. However, the mechanisms underlying these various effects are not fully understood. Therefore, we examined whether the GLP-1 analog liraglutide protects human umbilical vein endothelial cells (HUVECs) from the injurious effects of the proinflammatory cytokine tumor ...

Article 1

This article is basically a review of microRNAs and their role in cancer. It briefly introduces miRNAs and focuses on miRNA biogenesis and mode of action. It then looks at the functions of miRNAs with respect to their involvement in cancer, and their potential use in clinics. My literature review topic is about the role of miRNAs in tumorigenesis while this article is about the role of microRNAs in cancer. These two topics are technically the same. My review topic therefore shares a lot in common with this article, such as the miRNA biogenesis and mode of action and the ...

Reflection Questions

Question Number One:-

a) Sexual reproduction is defined as the formation of new organisms following the coming together of two independent gametes. Its main advantages includes the ability to produce wide ranging amounts of genetic diversity and ability to establish variable offspring’s that are different from the original parents, hence having high probability of survival in the changing environments . Other notable advantages include the ability to be reproduce offspring’s that develop from two parents ,hence reduced chances of inheriting deleterious mutations.

b) In my own perspective, I tend to ascertain that genetic testing to select embryos with certain traits ...

Introduction

This paper discusses Lassa fever, an infectious disease with regard to its causality organism, transmission, epidemiology, and factors causing the illness, clinical manifestation and diagnosis and the role of the nurse in controlling the illness. Lassa fever is a severe viral hemorrhagic infection caused by Lassa virus, which is affiliated to arenavidae virus family. Sierra Leone comprises of approximately 90% Africans of various tribal groups. The Temne people occupies approximately 30% of the total population in Sierra Leone and reside in the Northern part of the country. The current prevalence rate of the infection in the entire west Africa is ...

I. Abstract Fasciola gigantica and Paramphistomum cervi have been discovered to be composed of three important types of proteins these structural proteins that make them more adapted to their habitats and increase their immunity against their destruction by antibodies. This project is aimed at identifying these proteins contained in these two fluke genera using an analysis of soluble protein that elicit an immune response. II. Introduction

Biological research has shown that proteins like phosphagens contain high amounts of phosphates that release high amounts of energy that allow muscle movements like contraction and expansion. Other proteins like phosphocreatine ...

I. Introduction to exercise

Every year, nearly over 300,000 deaths are accredited to inappropriate diet and devoid of exercise, and a sedentary lifestyle has for long been recognized as an autonomous risk dynamic for cardiovascular disease. Even though physical dormancy escalates risk for both morbidity and all-cause mortality, it is one influence wherein alteration can create dramatic enhancements in health. Systematic exercise has been revealed to develop lipid aberrations, diabetes mellitus, obesity and hypertension control, with the paramount benefits recognized by sedentary persons who start to exercise (Braith et.al, 1995). Nonetheless, reactions to exercise interventions are regularly extremely adjustable among persons, and research ...

Introduction

Biology is broad and therefore embraces various topics in life that concern living organisms. Micro biology for instance deals with the study of very small organisms that are able to reproduce and sustain their generations. This is where the study of prokaryotes comes into the scene. The contrasting organisms to prokaryotes are referred to as eukaryotes. Contrary to prokaryotes, eukaryotic organisms have complex cell structures. Biology tends to full describe the complete nature of these organisms, their feeding habits, how they reproduce and where they thrive best, that is, their habitats.

Prokaryotes are single celled organisms whose cells lack ...