Essays on Plasmid

The advances in biotechnological field have made it possible to understand the genetic role behind molecular mechanisms to large extents. This in depth understanding of biological processes has paved ways to treat the untreatable aiding the human existence in multitudes of aspects like food, health, bioremediation, mining, biopharmaceutical production and much more. This microbial world is vast and has been a platform for human beings to explore their biological systems as they are simpler cells and can be manipulated with much ease than complex systems. The DNA harnessed within the cells are the molecular entities that have been targeted ...

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Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

Commercializing Biotechnology in Early-Stage Ventures.

Introduction: Ajinomoto Althea, Inc. is a drug manufacturing and contract development organization that provides clinical drug manufacturing and process development services to various global pharmaceutical and biotechnology firms. The company claims to be capable of fully supporting early stages clinical requirements via commercial manufacturing.

History and Background Information:

Althea Inc. was founded in 1998 by Francois Ferre, Ph.D. and Magda Marquet, Ph.D. and its first products were gene quantification assays and plasmids kits. The company delved into manufacturing microbial biologics in 2000, and by 2003, the company was now offering finish and fill manufacturing services to meet client demands. In 2008, due to ...

Major Achievements in Biology

Researchers and scientists are always intrigued by biological processes and activities, and strive to utilize them for benefit of mankind. Antibiotics, monoclonal antibodies, vaccines and several therapeutic agents, high yielding as well as disease tolerant plants and animals are all results of extensive research in the fields of cell and molecular biology, immunology, biotechnology and genetic engineering. Treatment to several diseases, even genetic disorders are available today only due to breakthrough discoveries in biology. Three such important discoveries are revelation of structure of ribosomes through X-ray crystallography, identification of tumor specific antigens (TSA) expressed on the surface of cancer ...

Abstract

Transformation remains, and hugely significant tool in extending the borders of life sciences and medicine. It can be referred as fixing intracellular DNA into a bacteria or organism through the vector, thus transforming it. This paper uses plasmid pUC18, self-reproducing circular/spherical strands of genes that may be modified. After the bacteria absorb the vector, some proteins not usually synthesized will occur. In this study, a system has been developed to monitor the transmittance of different DNA (genes) from a genetically transformed strain of Saccharomyces cerevisiae to E. coli. This mechanism is centered on yeast strains that contain several integrated ...

Panther I.D.:

“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids” Abstract The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...

Virulence and Pathogenesis, Treatment and Prevention

InstitutionVirulence and Pathogenesis, Treatment and Prevention of Opportunistic Bacterial Infections We live in a day that was much like 100 years ago, before antibiotics existed at all. The reason for this is that almost as soon as a new antibiotic is introduced, bacteria develop resistance to it. In 2008, a type of bacteria was found in China that has 45 specific genes meant solely to confer resistance to antibiotics just in its plasmid. Plasmid is a cellular material generated by bacterial colonies that are hard to penetrate with antibiotics, even without resistance genes (Lutz, 2016). As the search for ...