Rocky mountain spotted fever is tick-borne infection. In endemic areas, the prevalence of the diseases is 15 per 100,000 persons. The disease is characterized by sudden onset of fever and headache. It is caused by gram-negative intracellular bacteria Rickettsia rickettsii. The bacteria have a high predisposition for the endothelial cell. Actin protein polymerization in the cells is important for the spread of bacteria from one cell to another. (Raoult & Parola, 2007) The bacteria cause lymphohistiocytic vasculitis and necrosis of the infected cells. It typically takes 5-7 days from the day of tick bite to the onset of symptoms. ...
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During the second week, the CRP analysis was performed in the class. Blood testing for C-reactive protein aids in the rapid identification and verification of the progress and effectiveness of the treatment of infections, injuries and inflammation. The analysis was conducted to determine the acute infectious diseases, to monitor the effectiveness of treatment of chronic diseases (amyloidosis), monitor the effectiveness of antibiotic treatment (pneumonia, septicemia, neonatal sepsis, meningitis, etc.) (Arinzon Z. et al., 2011, p.365). In addition, it helps to define the determination of the risk of cardiovascular events in patients with atherosclerosis, diabetes but also on chronic hemodialysis ...
Major Achievements in Biology
Researchers and scientists are always intrigued by biological processes and activities, and strive to utilize them for benefit of mankind. Antibiotics, monoclonal antibodies, vaccines and several therapeutic agents, high yielding as well as disease tolerant plants and animals are all results of extensive research in the fields of cell and molecular biology, immunology, biotechnology and genetic engineering. Treatment to several diseases, even genetic disorders are available today only due to breakthrough discoveries in biology. Three such important discoveries are revelation of structure of ribosomes through X-ray crystallography, identification of tumor specific antigens (TSA) expressed on the surface of cancer ...
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Transformation remains, and hugely significant tool in extending the borders of life sciences and medicine. It can be referred as fixing intracellular DNA into a bacteria or organism through the vector, thus transforming it. This paper uses plasmid pUC18, self-reproducing circular/spherical strands of genes that may be modified. After the bacteria absorb the vector, some proteins not usually synthesized will occur. In this study, a system has been developed to monitor the transmittance of different DNA (genes) from a genetically transformed strain of Saccharomyces cerevisiae to E. coli. This mechanism is centered on yeast strains that contain several integrated ...
Panther I.D.:
“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids” Abstract The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...
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