Essays on E. Coli

Introduction

The situation is getting out of control at Good Health Hospital (Tampa Bay) since the outbreak of Escherichia coli, a specie of bacteria that can be found in many daily items. The incident involved Ward 10, which is located in the second floor. A recommendation from the Centers for Disease Control and Prevention (CDC) stated that the investigation of the bacterial source must begin with the hospital kitchen as the bacterium can be transmitted through contaminated vegetables and delicatessen meats (e.g. salami and other cold cuts). The Epidemiological Summary provides a brief description of the data obtained. Environmental review ...

ABSTRACT

There are so many things in the world that can make people very sick and in some cases can claim their lives. That being said if there are ways to avoid sickness then it should be embraced. Foodborne illnesses are all too common in modern society. Again, they are very common, but also highly preventable. If businesses and the general public dedicate themselves to greater efforts in food safety then these illnesses would never really be a threat. Understanding foodborne illness better it is best to look at some of the most common sources of foodborne illness, including Salmonella, ...

Between 19th October and 1st December 2015, the CDC reported an outbreak of the Escherichia coli O26 (STEC 026) in Delaware, California, Illinois, Oregon, Kentucky, Maryland, Minnesota, Washington, New York, Pennsylvania, and Ohio. The CDC linked the outbreak to Chipotle Mexican Grill restaurants.

The intestinal symptoms of E. coli infection show up within the first five days after infection and may include:

Loss of appetite Gas Nausea Vomiting Fatigue Sudden watery diarrhea that may turn to bloody stool Cramping of the abdomen Fever In severe cases, the skin becomes pale, urine in blood, a decrease in urine output, and dehydration (Pietrangelo and Kim). Infected people per state include Another short outbreak of a different strain of STEC O26 ...

Indwelling Catheter-Associated Urinary Tract Infection

In the article, Poudel, Baniya and Pokhrel (2009) conduct a prospective study to investigate the microbiological profile as well as the resistance pattern of the strains that cause urinary tract infection associated with the use of Foley’s Catheter. In the study, 100 catheterized adult patients, all above the age of 16, in a hospital participated. The patients used the indwelling Foley’s catheters for both short and long intermediate purposes. 3 ml urine samples were aseptically taken from the patients at the repeat of the catheterization and repeated on unevenly in subsequent days and weekly until the patient ...

Average velocity

The average velocity reported for microtubules propelled by kinesin expressed in insect systems is much higher than that reported for kinesin expressed in E. coli with a calculated mean and median of approximately 2.9330μm/s and 2.7974μm/s respectively for kinesin expressed in insect systems and 1.1579μm/s and 1.0903μm/s respectively for kinesin expressed in E. coli. This result is similar to result obtained by Korten et al. (15: 64) and Agayan et al. (29: 2265–2272) and the lower velocity observed in microtubules propelled by kinesin expressed in E. coli is likely due to the presence of ...

Please insert your name here

Please insert your professor’s name here Please insert course number here Introduction Kinesins are proteins which serve as molecular motors for the directional transport of various cargos (Hirokawa et al. 10: 682-696). They perform this important function using different mechanisms to recognize, bind, determine the direction of transport and unload the cargos with an energy efficiency which can exceed 50%, thereby equipping them to play important roles intracellularly in the transport of organelles and vesicles, in biosensing as well as in the development of other bioengineering solutions (Agarwal 11; Hirokawa and Takemura 301: 50–9; Hirokawa et al. ...

Food.inc is a movie produced by Jeff Skoll and Diane Weyemann in 2008. It's a documentary move. The purpose of this movie is to make people aware of what they eat and to expose the dark side of food industry. It shows how the food industry changed and how it changed farmers two. The movie also shows us the animal mistreatment. A chicken in 2008 is twice bigger than a chicken in 1950. It’s growing bigger and faster than a chicken in 1950. In 48 days the chicken will be bigger than a 70 days chicken in 1950. ...

Application of Epidemiology – Case Study

Application of Epidemiology – Case Study

Information Summative

The statistical analysis of the Good Health Hospital’s medical records reveals the progression of Escherichia coli infection among four hospitalized patients who reported bloody diarrhea, abdominal pain, vomiting and nausea, loss of appetite, flatulence and pyrexia since last week. The summary of these cases presented below in the context of their further analysis. The findings by the CDC revealed that the E. coli manifestations emanated among the affected patients after consuming the spoiled food items attributing to meats and vegetables.

Background Information (Indicating the Requirement of Further Investigation)

Georgia Department of Public Health (GDPH) describes various nosocomial infections reported within a ...

News that the man who was supposed to recover the company Chiptole was arrested on the charges of drug possession does not surprise me to a certain level. The reason for this is that he had a lot of burden on his shoulders and was responsible for regaining the good ratings and the customers’ loyalty after the outbreak of E. coli. According to the article (2016), “Chipotle saw sales at established locations drop 30 percent in the first quarter of the year“ (Choi). The executive had to regain the trust of the customers and even though that was his ...

{Author Name [first-name middle-name-initials last-name]} {Institution Affiliation [name of Author’s institute]}

Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

Introduction

E,coli bacteria is a commonly found within the human colon and it is a rod shaped enteric bacterium that grows in ideal conditions of 370 C. The bacterium can be cultured or grown in liquid or solid culture media within the lab. When grown in the ideal conditions, say LB broth, they tend to take a specific pattern while when grown in large amount of media they tend to take a lag phase where growth is minimal or none at all (Ding, Rahman, Purev & Oh, 2010). In the ideal environment, the bacteria tend to grow exponentially with their ...

Abstract

Transformation remains, and hugely significant tool in extending the borders of life sciences and medicine. It can be referred as fixing intracellular DNA into a bacteria or organism through the vector, thus transforming it. This paper uses plasmid pUC18, self-reproducing circular/spherical strands of genes that may be modified. After the bacteria absorb the vector, some proteins not usually synthesized will occur. In this study, a system has been developed to monitor the transmittance of different DNA (genes) from a genetically transformed strain of Saccharomyces cerevisiae to E. coli. This mechanism is centered on yeast strains that contain several integrated ...

{Author Name [first-name middle-name-initials last-name]} {Institution Affiliation [name of Author’s institute]} Urinary tract infection (UTI) is the most widespread problem, especially among women. It is observed the second most common incidence in primary and secondary care that requires antibiotic treatment (Shah & Goundrey-Smith, 2013). The UTI incidences are high among women and around 50% women face it once in their lifetime. The statistics suggest that women are more prone to UTIs in comparison to men and data shows that 7-8% women between the age of 60-80 and around 20% women above the age of 80 years are more susceptible ( ...

Panther I.D.:

“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids” Abstract The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...

Effect of Chipotle’s new strategies

Effect of Chipotle’s new strategies

Introduction -

Chipotle has so far secured the industry leader position by positioning itself as a food chain that sources fresh products and natural ingredients. The recent E.coli outbreak that made 55 people sick led to drop in sales of the organisation. The company is now taking steps to strengthen its market position and survive competition (Newman & Jargon, 2016).

Porter’s Five Force Model Analysis of Chipotle

Threat of new entrants - (High) - In the fast casual food industry the threat of new entrant is very high as there are less entry barriers and a food business requires low start-up costs. The ...

E COLI O157

WHAT IS ECO157? Enterohemorrhagic Escherichia coli O157 or otherwise known as “EHEC O157” is also called as the “verocytotoxin” which produces “shiga toxin” and the end result is EcO157 (Islam, et al., 2014, p.1). The E coli O157 has been reported as a food-borne pathogen known world-wide. One of the harmful effects of E coli O157 is that it can create hemorrhagic colitis that carries severe sequelae such as the hemolytic uremic syndrome as the product of the “shiga toxin” that came from the organism that can act on the human kidney, intestine and other parenchymatous organs (Islam, et ...

Introduction

Bacterial meningitis is an infection that affects the meninges, the cerebrospinal fluid and the spinal cord that causes death and disability in a number of people worldwide. This is a highly contagious airborne disease that is spread through the transmission of three organisms present in the respiratory secretions: Neisseria meningitides, Haemophilus influenzae, and Streptococcus pneumoniae. The onset or origins of the disease varies among different age groups and geographic locations. According to estimates, 200,000 people die annually of meningitis. Case fatality rates vary with age at time of illness and also depend on the particular bacterial strain causing the infection ( ...

(Institute Affiliation) Ethics is an integral part for any organization as all organizations deal with human beings. Ethical theories are put in place by the system so as to protect human and animal rights. This paper discusses the ethical theories that are prevalent in the agriculture and food industry. The purpose of this ethical code of conduct is to protect the animals, the workers and the consumers. There are regulatory bodies such the FDA and the USDA that were set up to ensure that the companies followed a particular code of conduct. The paper analyzes the popular documentary Food ...

[Professor]

Introduction Food Inc. is a documentary film that is personally enlightening. As a common trait among the American population, I order the fast food more often without realizing how it affects my personal health. After watching the film, I felt remorse for myself and for ever negative impacts caused by the society that has given met much comfort. The film tries to confront how food business is affecting health and environment and reveals who really benefits from it. It showcased celebrated law suits against the giant fast food chain in the United States due to its health impacts that ...

Before the establishment of the food safety agencies the food industry was largely self regulated. This led to problems where some manufacturers did not provide the minimum safety leading to cases of food borne illness. After dealing with these problems, laws were enacted which led to the establishment of the food safety agencies. After the establishment of the agencies that monitor food safety, the situation on the ground has improved dramatically. The United States has one of the strictest food safety policies, which has resulted in very high standards of food safety. The United States is a global leader in ...

Chapter 1: The Founding Fathers

The chapter provides a historical backdrop of Schlosser’s discussion and provides a rather nostalgic look into the post-World War II era, a time when the economy was good and citizens were achieving their dreams. The founding of the Taco Bell is used as a reference point to discuss how the white Americans conjured up the images of Mexico. Suggestions are made on a leadership conspiracy to put the Mexican-American youths in jail in the mid-1940s for a false murder accusation.

Websites

- www.whistleblower.org – reports on corporate ethics - www.hsus.org – Humane Society that defend animal welfare. - www.hrw.org/reports/2005/usa0105 – watches the safety operations of the ...

Introduction:

In 1976, Marion Bradford designed the Bradford method that is widely used in measuring the concentration of protein in a solution. When Coomassie Blue is added to a solution containing protein, the solution colour changes from brown to blue and this is attributed to the formation of a protein complex. The concentration levels of protein can therefore be measured by observing the dye color, which fully absorbed at a certain wavelength. The standard curve is used to calculate the concentration of protein because it represents the known BSA (bovine serum albumin) dilution. (Harvard, 2012) A study by Daiet (2008) analysed murinae in ...

Lab partners

The transformation of bacterial cells using Puc18 plasmid as a vector Abstract In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...

Introduction and Background

In assessing the impact of microbes in the New South Wales (NSW) health system, it was discovered after laboratory testing evaluating Part A and B categories different types of colonies were discovered, for laboratory 1 evaluations. In laboratory 11specimens used in this experiment were blood, urine, cerebrospinal fluid; sputum, swabs from and

Mucous membranes.

Bacteria colony morphology:-. E Coli. This bacterium appeared to be creamy, shinny and rose to a level of 2-3 mm. M Luteus. This one was yellow shinny circular and rose to 1-2 mm. Fungal colony morphology; Saccharomytes cerevsaie ( yeast) and Aspergillus nidulans ( Mould ). Precautions must be taken while ...

HEALTH RISK ASSESSMENT

Health risk assessment in humans is used to approximate the type and likelihood of the occurrence of health effects on people that may be exposed to hazardous material in a contaminated environment. Not only does the assessment seek to establish the likelihood and type of effects but also whether there is a given concentration of the hazardous material that would not cause any harm to humans. The assessment also involves an analysis of the chances whether the effects of the hazardous material will be different in people based on their sex, age, or genetics (Glendon, Clarke & McKenna, 2006, p. 18). A ...

Introduction

Ice cream, a milk-based good, is an excellent medium for microbial growth owing to high nutrient value, virtually neutral pH value (~pH 6–7), and elongated storage interlude of ice cream. The components of ice cream may be of a diversity of amalgamations of milk, cream, condensed milk, dried milk, colouring materials, flavours, fruits, nuts, sweetening agents, eggs, and eggs products, and stabilizers. Any of these could add to microorganisms’ growth and influence the quality of the goods as evaluated by its bacterial load or the presence of different explicit bacteria species. Time reliant heating throughout the ice cream creation decreases ...