Sometimes Forensic Casework items consist of less than 100 picograms (pg) of template DNA which, if we assume 3.5 pg of DNA per haploid cell, can be equated to approximately thirty haploid cells or fifteen diploid cells are termed as LCN DNA (low template DNA or LT DNA). (Gill 17) Such low copy number (LCN) samples exist due to several factors like damaged or degraded DNA, oligozoospermia or aspermic perpetrators or extended interval post coital samples, where there is loss of sperm over time because of the effects of drainage or host cell metabolism. From crime scenes, Trace ...
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