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Essays on Boric Acid

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Extraction Of Plasmid DNA Using Alkaline Lysis Method And Analysis By Agarose Gel Report

ABSTRACT:

A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...

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Concentration
Gene
Enzyme
Water
DNA
Genetics
Thinking
Buffer
Gel
Restriction
Electrophoresis
Tube
12 Pages

Standard Operating Procedure For Fasterbettermedia LB Essay Example

Principle Gel Electrophoresis is a method used in the laboratory for the separation of charged molecules like nucleic acids (DNA and RNA) and proteins that differ in size or charge (Agarose gel electrophoresis (basic method)). LB (Lithium Borate) buffer is the widely used buffer in agarose gel electrophoresis. The buffer solution comprises of Lithium hydroxide monohydrate and boric acid. LB buffer has low conductivity which can run at a high speed (5-50V/cm) than the gels which are made of TBE or TAE buffers (5-10 V/cm) which in turn reduces the run time drastically (Brody 6).

Experimental Required

Materials: Gel Electrophoresis Unit (Gel casting ...

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ADHD
DNA
Gel
Buffer
Electrophoresis
Running
Apparatus
Solution
Acid
Turn
Preparation
Power
2 Pages
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