Introduction
Spectrophotometry is one of the most common and efficient means of chemical analysis currently used in estimating the concentration of some chemicals. The technique exploits the radiation absorption property of various chemical substances in determining their concentrations. Spectrophotometry is based on Beer-Lambert Law. Beer-Lambert Law is the linear relationship between the absorbance and concentration of an absorbing species (Nair, 2010; Burrows et al., 2013). The mathematical expression of Beer-Lambert Law is shown in equation1 below: A=aλ*b*c Equation 1 In the equation 1 shown above, A represents the measured absorbance, a is the absorptivity coefficient which depends on ...
