Essays on Nitroaniline

4 – Nitroaniline

INTRODUCTION

4-Nitroaniline is an organic nitro aromatic compound. It is a yellow powder with a pungent odor and is highly soluble in hot water, methanol, ethanol, acetone etc (Lide 1994). Its chemical formula is C6H6N2O2. It has the following chemical structure: (Courtesy: PubChem 2016) It is also known as P-Amino nitrobenzene, P-Nitraniline or Benzene amine (PubChem 2016). It explodes on decomposition to emit toxic nitrogen oxides (Lewis 1996). 4-Nitroaniline is used in the manufacture of textile dyes such as the red azo dyes. It is also used in preparation of antioxidants, fuel additives, pesticides, pharmaceuticals and poultry medicines ( ...

The absorbance measured using standard samples were as shown in Table 1 below.

The absorbance measured in determining trypsin rate of activity in the absence of inhibitor was recorded in Table 2 below.

The absorbance measured in the determination of trypsin in the presence of inhibitor was recorded in Table 3 below.

The results of absorbance of the different standard samples were used to plot a graph of absorbance against p-nitroaniline concentration as shown in Figure 1 below. Figure 1: A standard curve on the relationship between absorbance and p-nitroaniline concentration Figure 2: Relationship between absorbance and p-nitroaniline concentration Figure 3: Relationship between rate of reaction and p-nitroaniline concentration in the presence of an inhibitor

Reciprocal of the data used to plot Figure 2 and 3 was calculated and used to plot Figure 4 and 5 respectively.

Figure 4: Lineweaver-Burk plot in the absence of inhibitor

Determination of Vmax and Km was conducted as follows:

y = -302.55x + 514.35 1V = ...