Dihydrofolatereductase (DHFR) is an important enzyme in the formation of DNA (Goodsell, 2002). DHFR controls the amount of tetrahydrofolate in the cells, which in turn is an important cofactor for purine and thymidylate synthesis and ultimately in the growth and proliferation of the cell (Davidson &Sittman, 1999). The enzyme is common to many species on earth. Human and bacterial DHFR have a relatively low rate of primary sequence agreement at 26%. However, they both retain a common general structure.The key role that the enzyme plays in biology as well as the variations that the enzyme displays makes it an important target ...
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