Analysis of Culture Cell KU812F with Three Protocols
Abstract
Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...
