Introduction:
In 1976, Marion Bradford designed the Bradford method that is widely used in measuring the concentration of protein in a solution. When Coomassie Blue is added to a solution containing protein, the solution colour changes from brown to blue and this is attributed to the formation of a protein complex. The concentration levels of protein can therefore be measured by observing the dye color, which fully absorbed at a certain wavelength. The standard curve is used to calculate the concentration of protein because it represents the known BSA (bovine serum albumin) dilution. (Harvard, 2012) A study by Daiet (2008) analysed murinae in ...
