Calculations for ΔG and KeqΔG=-NFECELL=-296.4850.45=-86836.5ΔG=-RTlnKEQ-86836.5=-8.3144293lnKEQlnKEQ=-86836.5-2436.1192lnKEQ=34.65e34.65=KEQKEQ=3.545 Based on the numerical value for Ecell (measured), the reaction is spontaneous. Being that the redox reaction has a positive value for Ecell, the reaction is spontaneous. It would cease to be spontaneous if the Ecell value is negative (Aus-e-tute, p.1). Based on the numerical value for delta G (calculated), the reaction is spontaneous. Delta G, or Gibbs free energy can have a positive, negative or null value. A reaction is non spontaneous when delta G is positive, and spontaneous when the value is negative. A reaction is said to be at ...
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Lab Report: Ethanol Determination Experiment
Objective: “To Determine the Ethanol content in several beverages using the enzyme alcohol dehydrogenase.”
The Chemistry behind this experiment is simple enough to understand. In the reaction carried out in the experiment, Nicotinamide Adenine Dinucleotide (NADH) is formed which can be analyzed quantitatively to find the concentration of Ethanol. The reaction involves oxidation of Ethanol to Acetaldehyde. This oxidation takes place with concomitant reduction of NAD+ to NADH. This formation of NADH can be seen in the Δ absorption at 340 nm micro level. At this wavelength, the reduced co enzyme absorbs at a very fast rate than the oxidized one, ...
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