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Use of Restriction Enzymes in Pharmaceuticals Restriction enzyme, which is also referred to as restriction endonuclease, is a class of enzyme that is helpful in cutting DNA at restriction sites. Restriction sites are particular recognition nucleotide sequences on double stranded DNA having the length of 4-5 base pairs. Restriction enzymes are also known deoxyribonucleases (DNases). Presently, there are four different types of restriction enzymes: - Type I enzymes that are “classical” in nature, e.g. EcoKI, EcoBI, and EcoR124; - Type II enzymes that are “orthodox” in nature, e.g. EcoRI, HindIII, EcoRV; - Type III enzymes ...
