Essays on Denaturation

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Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

I. Introduction Advances in recombinant DNA technology and bioinformatics-based data analysis have driven the explosion of structural biology in the last two decades (Kelly, Jess, and Price 119). This has resulted to the identification of at least 2000 proteins annually and a growing need in performing structural studies in these new proteins. The current trend involves the concern of analyzing protein structures close to its natural conditions (and other conditions) and of measuring their rates of structural changes, an essential factor in their biological functions. The technique of circular dichroism (CD) has gained an increasing popularity in addressing these ...