Essays on EDTA

Introduction

The restoration of the Sistine Chapel, perhaps one of the most famous examples of western art by Michelangelo began in 1965 as an initiative by Deoclecio Redig de Campos, who would later become the Vatican Museums’ general director in 1971. The scientific aspects arising in this project would be handled by Fabrizio Mancinelli as well as Gianluigi Colalucci, Maurizio Rossi, Bruno Baratti and Giorgio Bonetti master restorers. The cleaning of these frescoes would include only the depictions of the scenes with Jesus and Moses in the fifteenth-century paintings. At this time, the two 16th century frescoes located at the ...

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ABSTRACT:

A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...