- Cell Culture and Cell Lines: Three breast cancer cell lines: CAL120, EVSAT and MDA-MB-231, were cultured individually, in RRMI 1640 supplemented with 20% Fetal Bovine Serum (FBS), 100U/ml Penicillin, 100U/ml Streptomycin and L-Glutamine. The cells were cultured and maintained in an incubator at 370C supplied with 5% CO2. For subsequent experiments, the cells were further cultured till confluence, in 6 well and 96 well plates using RPMI+20%FBS. 1.2 Treatment of Cells: For treatment, 1x106 cells were seeded in 6 well culture plates, and incubated for 24 hrs. After 24 hours, the old media ...
Cancer Cell College Essays Samples For Students
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Environmental Hormone Mimics
Hormone mimics are types of chemicals in the environment that mimic the natural hormones in the body. Wagner and Oehlmann (1999) list three pathways xenohormones (foreign hormones) enter the body. When the xenohormones enter the body it is called “xenohormones uptake.” They are (1) breathed in from the air, (2) absorbed from body lotions, perfumes and deodorants, and (3) eaten with food that has been stored in containers or plastic wrap containing the chemicals. Estrogen, androgen and thyroid hormones are “chemical messengers” made by glands (like the thyroid gland and adrenal gland) to regulate different processes in the ...