Essays on Nuclease

Introduction

The treatment for multitudes of diseases and disorders resulting from genetic reasons have been ambiguous since long as the only cure could be the correction of genes and DNA in the specific region. Gene therapy gave a promising horizon to the correction of such genetic defects whereby molecular tools are applied to reverse the mutation or any other changes in the DNA. The advancing biomedical research have offered several tools for gene therapy since last few decades that have been able to perform the desired roles but with one or the other limitations. Lately, CRISPR-Cas9 based tools for gene ...

Gene Editing Techniques

1. Gene Editing – General Background Gene editing entails inserting, deleting and replace the DNA in a genome of an organism utilizing engineered nucleases. Sections of the natural gene are replaced and completed using a synthetic DNA chain. Also, the natural repair process gets rid of the mismatches and gaps in the DNA. On the other hand, gene disruption is a technique by which the DNA fragment is utilized to replace a genome sequence with a chosen marker gene like the kanavanine resistance. DNA –binding proteins are proteins that consist of DNA-binding domains and hence have affinity for single and ...

Introduction

Trichomonas gallinae causes avian trichomoniasis infection, which is often a lethal disease. The parasite is considered to primarily affect columbiform birds like pigeons and doves, but can affect other wild and domestic birds as well (Stabler, 1954). Diagnosis of the infection can be reliably done by collecting and studying material from the bird’s mouth and crop.

I carefully collected oropharyngeal swabs from the oral cavities of hunter-killed Woodpigeons in eight areas of Norfolk and one area of Suffolk counties in the three months’ time period between April and June. Using sterile cotton swabs, I collected crop swabs from the ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...