Panther I.D.:
“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids” Abstract The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...
