Essays on Agar

Abstract

Handwashing is an important process in controlling microorganism transmission and infectious diseases. The purpose of this experiment was to determine the effectiveness of different antibacterial hand cleaners (soap, alcohol hand sanitizer and ethanol soaked wipes) on killing bacteria. Hands were sampled without cleaning with antibacterial hand cleaner (control), and after cleaning with antibacterial soap, alcohol sanitizer, and ethanol soaked wipes. Samples were taken with a sterile cotton swab and inoculated on agar plates. Each experiment was done three times. Colonies were counted, mean values and standard deviation were calculated, and data was reported. Alcohol and sanitizer and ethanol soaked ...

INTRODUCTION

Klebsiella pneumoniae belongs to Klebsiella species of Enterobacteriaceae and constitutes the normal flora of the human mouth and intestine. K. pneumonia is the most predominant and clinically critical organism. Infections with K. pneumoniae are mostly contracted from the hospitals and principally occur in patients with damaged host defenses. The objective of this study is to perform techniques to identify the bacterium in the given sample and confirm its presence by staining and microscopic analysis.

Streaking may be performed on the clinical sample using Mac Conkey agar or blood agar with the goal of isolating colonies of the organism from ...

Abstract

Transformation remains, and hugely significant tool in extending the borders of life sciences and medicine. It can be referred as fixing intracellular DNA into a bacteria or organism through the vector, thus transforming it. This paper uses plasmid pUC18, self-reproducing circular/spherical strands of genes that may be modified. After the bacteria absorb the vector, some proteins not usually synthesized will occur. In this study, a system has been developed to monitor the transmittance of different DNA (genes) from a genetically transformed strain of Saccharomyces cerevisiae to E. coli. This mechanism is centered on yeast strains that contain several integrated ...

Panther I.D.:

“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids” Abstract The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...

QUESTIONS ON BIOCHEMISTRY

Questions on Biochemistry Describe the difference between an obligate and a facultative aerobe. Obligate and facultative aerobes are the same in terms of their ability to use oxygen in their respiration process. However, obligate aerobes requires oxygen to oxidize substrates such as sugar and fat to produce ATP (energy). Obligate aerobes are more efficient in converting substrates to ATP. The key process in aerobic respiration is the conversion of sugar to ATP shown in reaction 1 below:

In the reaction 36 molecules of ATP is produced per molecule of glucose. It is actually a slow process which comprises 3 ...