Essays on Hydrolyze

Introduction

Bacillus Subtilis are the organisms that are aerobic, Gram Positive, spore-forming belonging to the Bacillus genus. The organisms are together with other species that are closely related play a crucial role in disease, food poisoning as well as food spoilage. Lack of methods that are standardized for the identification of the organisms whether coming from the food or environment has made the tests to be difficult. Use of morphological as well as physiological tests have offered the best ways to identify organisms in the laboratory although there are inconsistent and lack of reliance in these methods. Identification of an ...

Abstract

Amylase from Bacillus Subtilis and Aspergillus oryzae were studied as representative candidates for bacterial and fungal enzymes, respectively. The activities of these two enzymes were studies comparatively over a period of 10 minutes and at temperatures of 0, 40, 60 and 95°C. The experiment was conducted to find the most effective temperatures for bacterial amylase and fungal amylase to catalyze starch hydrolysis. The data suggest that fungal enzyme was relatively more active, particularly in the first 6 minutes, than the bacterial counterpart at 0°C. On the other hand, the fungal enzyme activity deteriorated more rapidly than that ...

Introduction

An eEnzyme is kind of a protein and, like all proteins, proton is composed of a chain (or chains) made up of the 20 different amino acids found in most living organisms. The sequence of the amino acid chain(s) determines the 3-D shape of the protein, and its shape to a great extent determines its properties. and it has a 3-D shape. Enzymes act in aqueous solutions to facilitate used in biochemical reactions by lowering the energy barrier, but they are not themselves changed or consumed by the reaction. This is referred to as catalysis, and enzymes are biological catalysts. to make ...

Lab partners

The transformation of bacterial cells using Puc18 plasmid as a vector Abstract In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...