Essays on Subunit

Vid30 complex participation in the degradation of CDC25 in yeast and their role in Ras/ cAMP/ PKA pathway

Background: Unicellular organisms such as Saccharomyces cerevisiae in its total life cycle is exposed to diverse and changing nutrient conditions. Thus, in order to survive and achieve a competitive advantage, it has adapted itself such that it not only uses the wide range of available nutrient sources but also the sources that are nutrient rich. It mainly uses fructose and glucose by rapidly fermenting to alcohol. In response to these nutrient conditions, the intracellular trafficking transcription and turnover of protein from nutrient transporters (e.g. Hxts) get regulated. Therefore, when the availability of the glucose increases, the expression of low- ...

Major Achievements in Biology

Researchers and scientists are always intrigued by biological processes and activities, and strive to utilize them for benefit of mankind. Antibiotics, monoclonal antibodies, vaccines and several therapeutic agents, high yielding as well as disease tolerant plants and animals are all results of extensive research in the fields of cell and molecular biology, immunology, biotechnology and genetic engineering. Treatment to several diseases, even genetic disorders are available today only due to breakthrough discoveries in biology. Three such important discoveries are revelation of structure of ribosomes through X-ray crystallography, identification of tumor specific antigens (TSA) expressed on the surface of cancer ...

Introduction

Cloning is a term used to describe different methods of duplicating biological materials. Plasmids can be used to ‘clone in a gene’ and then express a given protein in mammalian cells. The type of protein used here is called AWP1 protein which is associated with PRK1. This type of protein is a universally expressed protein and the AWP1 gene is switched on during the early development of a being. The AWP1 may play a regulatory role in the mammalian signal transduction pathways. The AWP1 posses a conserved zf-A20 zinc finger domain at its C-terminal. When it is expressed in COS – 1 cells, the Myc-tagged ...