Antepartum testing seeks to prevent fetal death, especially in high-risk mothers. Thorp and O’Neill (2013) stated that the evaluation identifies risks for an intrauterine injury that may lead to death with the aim of offering timely interventions. Apart from this primary role, could the assessment be of benefit in human genetic engineering?
Although some scientist opines that genetic engineering in humans has positive effects on humanity, there are many adverse effects. For instance, human genetics tend to mutate over time. As a result, the choice of the “perfect baby” may not be a viable idea on the long-term ...
Cloning Reports Samples For Students
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The transformation of bacterial cells using Puc18 plasmid as a vector
Abstract
In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...
ABSTRACT:
A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...
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Abstract
The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...
Introduction
The discovery of restriction enzymes (REs), bacterial transformation, and agarose gel electrophoresis caused the sudden increase of popularity of molecular biology in the 1970s. Restriction enzymes allow the manipulation of specific sites of an organism’s genes and are commonly used in many laboratories worldwide. Restriction enzymes cut DNA in many different ways and can produce sticky ends and blunt ends (Weaver, 2012). The most abundant type of restriction enzymes is Type II, which require magnesium cations as cofactor (Pingoud et al., 2005). Aside from their popularity is recombinant DNA technology as a “cut and paste” tool, estriction enzymes can ...
Introduction
The most widely used model when investigating the breast cancer is T47D cell lines. These cell lines are easily spread, easily manipulated genetically and when used in the experiment, it provides good results. They have been used to examine new therapies and the and the breast cancer pathobiology1.
Over many years HER2 has 25% - 30% of human breast cancers have been caused by gene amplification. The monoclonal HER2 and the EGFR are the two HER2-targeted therapies that are used for approval in the most clinics. During growth, HER2 is used to regulate the cell growth and assist ...
Abstract
The diamond industry has gained popularity as well as the industry becoming a lucrative one for the selected few. A great advantage fell on those companies that were formed at the onset of realization of the value of diamond. De Beers was one among those that were lucky to strike gold with the rich deposits in South Africa. This realization made the company to gain dominion over the market as far as the amount of diamond in the market was concerned. With respect to this, De Beers was placed in a position in the market to influence the prices to their ...
