Essays on Base Pair

Choose a gene that has been implicated in a human developmental disorder

FOXP2 is a gene that codes for a member of the transcription factors’ forkhead /winged-helix family, FOX. It is situated from base pair 114,086,309 to base pair 114,693,771 on the chromosome 7’s long (q) arm at position 31. The gene has 42 distinct introns, which include 41gt-ag and 1 other. The first FOXP2 report depicted 19 exons, of which two, namely 3a and 3b, are variably spliced into transcripts that cover about 300 kb on the chromosome 7q31 (Lai, Fisher and Hurst).

Several alternative transcripts, as well as protein isoforms, have been predicted on the ...

Exam #1 (50 points)

1a ) Natural selection is the process by which adaptive traits of species increases through generations. In Cadotte’s research, he had several combinations of the 17 kinds of plots and he found out that those which had the most variation, survived the most. These were the plants with the most distance from each other. The adaptive traits of these plants were inherited by its offspring. Since the plants with traits that survived the most produced more offspring then these were the ones that flourished the most. The reason, Codette explained was that these plants had different needs therefore they ...

Introduction

The discovery of restriction enzymes (REs), bacterial transformation, and agarose gel electrophoresis caused the sudden increase of popularity of molecular biology in the 1970s. Restriction enzymes allow the manipulation of specific sites of an organism’s genes and are commonly used in many laboratories worldwide. Restriction enzymes cut DNA in many different ways and can produce sticky ends and blunt ends (Weaver, 2012). The most abundant type of restriction enzymes is Type II, which require magnesium cations as cofactor (Pingoud et al., 2005). Aside from their popularity is recombinant DNA technology as a “cut and paste” tool, estriction enzymes can ...

- Ingestion of fava beans increases the formation of free radicals in red blood cells which in turn causes disruption on the normal functioning or reduction of Glucose-6-Phosphate Dehydrogenase enzyme. Consequently, glucose-6-phosphate dehydrogenase reduces and therefore the red blood cells are incapable of defending themselves from the detrimental effects of reactive oxygen species. This makes the damaged cells to breakdown and rupture prematurely hence hemolysis.

Glucose-6-phosphate dehydrogenase pathway

The G6PD gene is located from base pair 153,759,605 to base pair 153,775,795 on the X chromosome. - Fatty acid synthesis occurs in the cell cytoplasm. Fatty acid synthesis ...

Abstract

A-kinase anchoring proteins (AKAPs) are a group of structurally varied scaffold proteins which are found to regulate the PKA pathway by localizing the protein kinase A and some specific enzymes at sub cellular level. So far several of the AKAPs have been found to play critical role in the regulation of phosphorylation of several PKA dependent substrates which are necessary for the modulating the cardiac function. Abnormalities in these AKAPs genes have been found to cause various forms of cardiac disease. The most prominent AKAPs are the AKAP9 and AKAP13. Mutations in the AKAP9 gene has been found to be ...

Determining the size of gene fragments

1) Alul restriction enzyme cuts the protease gene at restriction sites with base position 62 and position 1149 respectively. Considering the fact that the gene has 1216 base pairs, three possible fragments are generated with Alul restriction digestion.

The first digestion fragment length ranges from base pair 1 to base pair 62= 61 base pairs.

The second fragment length ranges from base pair 62 to base pair 1149 in length=1087 base pairs The third fragment ranges from base pair 1216 to base pair 1149 in length= 67 base pairs.

2) Sa/l restriction enzyme cuts the protease gene at ...