Antigen Reports Samples For Students

Abstract

The aim of the experiment is to plot the calibration curve for deteremination of antigen 4D5 by performing ELISA experiment. The primary antibody was blocked by bovine serum albumin, then it reacted with IgG (secondary antibody). Then, the system was washed with carbonate, blocking and substrate buffers. Three replicate experiments were performed, and the experiment was performed with the serial dilutions. By measuring the optical density of the diluted solutions, the calibration line was obtained and the region with the linear dependence “concentration – optical density” was determined. The callibaraion line can be used for the practical tasks: for detecting ...

Introduction

Immunotherapy is a process through which diseases related to or rather affecting the immune system are treated by simply ensuring the capabilities of the immune system are improved.

Such is possible through various ways but the most common method of enhancing the immune system and ensuring its effective is through vaccination. This can also be made possible by eliminating or alternatively controlling some immune responses as evident when dealing with allergy issues. According to Edwin Smith Papyrus, treatment of most complex diseases was effected through surgery as it was considered the most effective method of treatment. This was mostly as ...

Introduction

In the mouse and other mammalian species, the pool of primordial follicles becomes established during fetal or neonatal life (Oktay et al., 1995). Over the mammal’s lifespan, the follicles undergo growth and development, and eventually the initial reserve of follicles begins to diminish during the mammal’s midlife. Briefly, follicular cells move from a primordial phase, in which little DNA synthesis is taking place, to enter a primary and then secondary stage, in which the granulose cells undergo intense proliferation and high levels of DNA synthesis can be observed. Antral and post- ovulation stages follow (Oktay et al., 1995). Folliculogenesis ...

Introduction

Mutation induces the formation mRNA strands that are formed later that they are converted into a native protein. Mutational causes the peptide formed develop a structure similar to a protein. Antimicrobial peptide formed after mutation depends on the chemical property. Mutation causes bacteria to increase in the peptide leading to low hemolytic function. Bacteria lead to anoplin to causing the shape of peptide to form a protein like shape that is spectroscopy.
Mutation causes the chain of the peptide to form a protein structure that later forms a tertiary that is the antigen molecule responsible for forming the body immune system. The chains amino ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract
Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

Introduction

- Discuss New Jersey State health initiatives and their relationship to the DHHS National Prevention Strategy and Healthy People 2020.

List the state-mandated reportable communicable diseases.

Discuss the status of measles cases in the state with accompanying epidemiologic data.
Discuss the status of pertussis cases in the state with accompanying epidemiologic data.
Explain the Organizational structure of the state’s official public health agency.
Present an overview of the state’s immunization program to include discussion of IIS (Immunization Information System).
Conclusion Summary
- Post of a summary of the paper to the Discussion Board in Blackboard with response to at ...

Report for product transporting

Introduction
Preparation of protein EcCLpS in lab environment requires much time as well as controlled environment. ClpS protein is a member of the Clp family of proteins displaying modular structure. It is essentially a bacterial adaptor protein. Artificially prepared CLpS protein is sought by companies, especially pharmaceutical companies, for research; especially for ELISA, western blot experiments, antibody production, Right-Over –Expression Lysate and Left Control. It is researched for its application as antigen standard for colipase. As it is a specialized production, care has to be taken in its handling and dispersing to the end client. Specific conditions need to be maintained ...

Introduction

In any blood bank laboratory, definite tests must be done on all donated blood. This incorporates typing to verify the donor's ABO blood grouping and Rhesus factors in addition to several screens to guarantee the safety of the blood. Screening is usually conducted for unanticipated red blood cell antibodies that could cause severe reactions in the recipient and bacterial contamination in components of platelets as well as present and precedent transmissible infections. Each unit of donated blood is usually tested for Hepatitis C, HIV, Human T-Lymph tropic Virus, Syphilis, West Nile Virus and Malaria. But before any test is ...