Essays on Antibody

Abstract

The aim of the experiment is to plot the calibration curve for deteremination of antigen 4D5 by performing ELISA experiment. The primary antibody was blocked by bovine serum albumin, then it reacted with IgG (secondary antibody). Then, the system was washed with carbonate, blocking and substrate buffers. Three replicate experiments were performed, and the experiment was performed with the serial dilutions. By measuring the optical density of the diluted solutions, the calibration line was obtained and the region with the linear dependence “concentration – optical density” was determined. The callibaraion line can be used for the practical tasks: for detecting ...

Introduction

The paper compares the financial performance of Abcam and Novozymes for the FY 2015. The company (Abcam) spends a lot of cash on research and development. Therefore, it provides easy access to products, high development on the platform of e-commerce. The sales of Abcam increased significantly between FY 2015 and 2016. That is it almost made 90% of sales in other currencies such as the Japanese Yen, and Euro. The change in these foreign currencies during the recession period is favorable for the firm. Moreover, revenues of the product increased by over 15% on the reported basis during FY ...

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Immunotherapy in Treating Human Cancer

The immune system of our body is a complex network of tissues, cells, and organs that act mutually to protect our body from unknown foreign trespassers. It recognizes the harmful particles entering the body and facilitates the destruction of those particles in order to shield the body system from any ailment. Due to any complexity or disturbance often the immune system targets the wrong particle considering it as a dangerous particle and this anomaly of the immune system may result in various autoimmune diseases like arthritis, allergies, malignancies, and AIDS (Understanding the ...

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Description of HIV

Human Immunodeficiency Virus (HIV) is a virus that weakens and destroys the immune system of the body so that the body cannot defend the infection. HIV positive person also possesses AIDS (Acquired Immunodeficiency Syndrome), and if HIV is treated at its early stage, the risk of AIDS can be averted. If an HIV-positive person is diagnosed with AIDS, it means that the complete destruction of the immune system has been accomplished by the HIV and that increase the vulnerability of the patient towards various opportunistic infections. The ...

Rocky mountain spotted fever is tick-borne infection. In endemic areas, the prevalence of the diseases is 15 per 100,000 persons. The disease is characterized by sudden onset of fever and headache. It is caused by gram-negative intracellular bacteria Rickettsia rickettsii. The bacteria have a high predisposition for the endothelial cell. Actin protein polymerization in the cells is important for the spread of bacteria from one cell to another. (Raoult & Parola, 2007) The bacteria cause lymphohistiocytic vasculitis and necrosis of the infected cells. It typically takes 5-7 days from the day of tick bite to the onset of symptoms. ...

Overview: Overview: Antigen-antibody reactions are commonly used in immunological assays. In a traditional agglutination assay, the end product is a visible antibody-antigen complex that settles as visible particulate matter in the solution. In other cases, the reaction is invisible and wants the use of a specific enzyme or isotopic probes that can amplify the signal created by the antigen-antibody reaction, so that they can be detected with the naked eye or an ELISA/RIA reader1. Hemagglutination is a visible antigen -antibody assay in which soluble antibodies react with antigen on the surface of (red blood cell) RBC to form a ...

Influenza Vaccine on the Elderly

Introduction Based on current evidence, there is an overwhelming response with respect to the clinical efficacy, safety, and feasibility of influenza vaccine in the elderly. The impact of the vaccine is observed in elderly patients with chronic diseases with an influence on reduced mortality and hospital admissions. Based on a recent publication, the influence of the vaccine is also observed among health elderly population around the globe. Researchers and healthcare providers state that the declining age is associated with poor antibody response coupled with various confounding factors. However, these factors have not been clearly stated in literature. On the ...

Abstract

Systemic lupus erythematosus (SLE), otherwise known as lupus, is an autoimmune disease caused by varying and often unknown sources. There are different types of lupus, with SLE being the most common. Other types include lupus nephritis, cutaneous lupus erythematosus, drug-induced lupus, and neonatal lupus. SLE can affect different organ systems including the skin, kidneys, liver, blood cells and brain. There is no direct known cause for this disease, but it is thought to be caused by a combination of factors such as certain medications, hormones, genetics and the environment. There are currently few treatments available for this disease and ...

Immune System

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Immune System

Introduction

The human immune system is a complex system consisting of a network of numerous cells and cell types, tissues, and organs working together to defend the human body from foreign bodies such as invading microorganisms, infections, and tumors, while ignoring the normal working and healthy parts of the body.

Discussion

Working of the immune system The immune system has different ways of identifying problems, communicating with other cells, and performing their activities. The immune system has the ability to distinguish healthy cells from unhealthy cells as it can recognize a number of different “danger” cues ...

Definition:

Ebola virus, well known for its hemorrhagic effects produced inside as well as outside of body. This virus progresses with bleeding, which leads to organ failure and if not treated properly can also cause death. ("Ebola virus and Marburg virus - Mayo Clinic", 2016). Ebola is also termed as Ebola Hemorrhagic fever because of its hemorrhagic property and it is also known to cause the death of 90% effected people ("Ebola Virus: Symptoms, Treatment, and Prevention", 2016). There are 5 strains of this virus and four of them are known to cause deadly symptoms like hemorrhage. Ebola have its ...

Major Achievements in Biology

Researchers and scientists are always intrigued by biological processes and activities, and strive to utilize them for benefit of mankind. Antibiotics, monoclonal antibodies, vaccines and several therapeutic agents, high yielding as well as disease tolerant plants and animals are all results of extensive research in the fields of cell and molecular biology, immunology, biotechnology and genetic engineering. Treatment to several diseases, even genetic disorders are available today only due to breakthrough discoveries in biology. Three such important discoveries are revelation of structure of ribosomes through X-ray crystallography, identification of tumor specific antigens (TSA) expressed on the surface of cancer ...

Myasthenia gravis (MG) is a type of neuromuscular disease that causes certain muscles to fatigue and weaken. The disease is considered to affect voluntarily controlled muscles such as those involved in chewing, facial expression, speaking, eye and eyelid movements and swallowing. The disease occurs in all sexes and age groups. However, it commonly affects women under 40 years and people aged between 50 and 70 years in both sexes, though it is known to affect older people at large (Sieb, 2014). Within the UK, it is estimated that in 100,000 people, there are 15 cases of the disease. ...

Flu is a viral disease that influences for the most part the nose, throat, bronchi and, once in a while, lungs. Disease typically goes on for around a week, and is portrayed by sudden onset of high fever, hurting muscles, cerebral pain and extreme disquietude, non-beneficial hack, sore throat and rhinitis (WHO, 2016). The name "flu" started in fifteenth century Italy, from a plague ascribed to "impact of the stars." The principal pandemic, or overall pestilence, that unmistakably fits the portrayal of flu was in 1580. No less than four pandemics of flu happened in the nineteenth century, and ...

Based on the initial symptoms, the patient most likely suffers from hypothyroidism or low thyroid hormone disorder. Further clinical tests and an examination of the family’s medical history reveals that the particular disorder could be Hashimoto’s disease; also called Hashimoto thyroiditis. Hashimoto thyroiditis is classified as an autoimmune disease. In autoimmune disease, one's immune system attacks the body’s tissue specifically the thyroid gland (Skugor & Wilder, 2006, p.2). By attacking the thyroid gland, the disease reduces the gland’s ability to produce thyroid hormones of substantial quantity. This condition leads to hypothyroidism. Thyroid hormone deficiency presents with ...

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Introduction

Lupus is a chronic autoimmune that can impact any part of the body resulting in a range of clinical symptoms. The affected part by Lupus may include brain, lungs, kidneys, skin, joints, blood vessels and other internal organs (Pisetsky, Gilkeson & St Clair, 1997). Lupus impacts the immune system by interfering with its capability of differentiating between antigens and normal healthy tissues. This incidence results in the misinterpretation by the immune system, and it directs the antibodies against the healthy tissues causing pain, tissue damage, and swelling. It ...

Immunology

The rearrangement of T cell receptor (TCR) genes g and d occur in series of cells expressing variable Vg and Vd gene segments. The lymphoid precursor cells originate in the bone marrow and move to the thymus. Preliminary T cells are devoid of TCR, CD4 and CD8 molecules and are called double negative thymocytes . These double negative T cells may be further categorized into four phases of differentiation (DN1, CD44+CD25-; DN2, CD44+CD25+; DN3, CD44-CD25+; and DN4, CD44-CD25-) . CD44 is a cell adhesion molecule and CD25 serves as the IL-2 receptor. Passage into the DN2 and DN4 stages ...

Introduction

The total number of patients with cancer keeps soaring globally with around 14 million cases diagnosed by 2014. Each year, about 8 million people die of cancer-related illnesses globally. There are quite some remedies that have been proposed to mitigate in the cancer menace. This study is focused on cancer treatment by curcumin through tyrosine kinase inhibition. Curcumin is the primary coloring agent found in turmeric, a tuber of the herb Curcuma longa Lin. It has widespread use as a flavoring agent (Aggarwal and Bokyung 87). It is also well known for its medicinal value commonly used to suppress ...

Multiple sclerosis is an autoimmune disorder, which affects the central nervous system. It was first described as a separate disease in 1868 by Jean-Martin Charcot (Compston and Coles, 2008). The average prevalence of the disease globally is 30 people from 100000 (Milo and Kahana, 2010). However, the incidence of multiple sclerosis varies greatly depending geography. While in the African countries it happens comparatively rarely, its frequency is increasing consistently in Asia and America. In Europe, multiple sclerosis happens with the highest rate in the world. There are four clinical forms of multiple sclerosis (Compston and Coles, 2008). Relapsing-remitting MS ...

Introduction and Overview

The Rhesus factor (Rh) refers to the red blood cell’s(RBC) surface antigen;the name Rhesus comes from the small Indian monkeys in which it was first discovered. Rh incompatibility,sometimes referred to as Rh disease, is a condition that occurs when a woman with the Rh-negative blood type is exposed to Rh- positive blood cells, leading to the development of Rh antibodies. This condition can have fatal outcomes including haemolytic disease of the foetus or newborn, autoimmune haemolytic anaemia and haemolytic transfusion reactions (HTRs). These conditions have, in the past, raised a lot of concerns, and some ...

RESULTS

Section 1 All samples were found to be positive for the anti-Ro antibody with higher than 240 IU/ml, except Sample ID # 13 and 32, which showed lower concentrations (Figure 1.). These samples also exhibited occurrence of Smd and Dn respectively, albeit at low concentrations. Sample Id# 12 and 20 also showed presence of anti-La antibody. The autosera of Patient Id # 32 suffering from SS displays a faint 52kd IgM band, which is representative of 52kd anti-Ro antibody and is fainter in ethanol treatment sample. Patient ID # 42 expressing a higher than 240 IU/L of anti-Ro antibody in the clinical ...

The American College of Obstetricians and Gynecologists, the American Academy of Primary Physicians, the American Academy of Pediatrics, and the World Health Organization recommend that mothers exclusively breastfeed their infants for the first six months after delivery (Stuebe). In fact, the World Health Organization states that even after solid foods are started at six months, the mother should continue breastfeeding her child for at least two years or as long as she is able to produce milk (Breastfeeding Action Group 1). These organizations are quite correct in issuing these recommendations for as opposed to formula milk, breast milk and ...

Lyme disease was identified in the 1975 and was named by the town Lyme, Connecticut, where a number of cases were registered. Experts say the disease existed long before the 20th century. “Scientists in Europe first knew and wrote about a disease like Lyme disease in 1883. German doctor named Alfred Buchwald wrote about a rash that looked like the bull’s-eye on a target” (Donnelly, 2000, p. 7-8). People did not know what caused these symptoms and used wrong treatment because of that. Today doctors have much more information about this issue. Lyme disease is a representative of ...

Mechanisms by which Incompatible Red Cells may be Cleared from Circulation

Introduction One of the leading causes of transfusion-associated mortality is related to haemolytic transfusion reactions.1 Many factors that account for haemolytic transfusion, and complement activation is the most widely studied and documented feature linked with such reactions and fatalities, and many researchers have focused on the role of complement initiation, activation and regulation with respect to these reactions. Others have focused on the underlying strategies involved in mitigating the complement modulation, in the event of an incompatible red blood cell transfusion.1 Many reports have stated that incompatible red blood cell (RBC) transfusions are the leading cause of death in ...

What is Hepatitis B?

Hepatitis is an infection which damages the liver organ known to be responsible for processing of different nutrients, filtering of the blood, and fighting of infections. Hepatitis B is a continuous type of hepatitis infection caused by a double-stranded DNA virus known as Hepatitis B virus (HBV) (Trépo, 2014). Hepatitis B infection can either be acute or chronic. Acute Hepatitis B occurs during the first 6 months of infection while Chronic Hepatitis B is the term used to describe the instance where the virus stays in the body and the person develops a lifelong disease (Centers for Disease ...

Hemolytic Disease of the Newborn

Hemolytic Disease of the Newborn (HDN) is a blood-related disorder that occurs in a fetus or a newborn infant. This problem usually arises, when a mother has different blood type than her unborn baby, i.e. a condition known as “incompatibility”. Most common form of this incompatibility is ABO incompatibility, which is not very severe condition, and the least common form of the incompatibility is Rh incompatibility. Due to these differences in blood types, antibodies in the blood of mother attack the red blood cells of the developing baby resulting in the destruction of blood cells of the baby causing ...

Introduction

Breast cancer is a genetic disorder so common that it has claimed the lives of many women all over the world with statistics showing that it’s concentrated on the age of 45 - 55 years. Breast cancer, along with the other category of this disease is the product of the occurrence of a metastasized tumor. Neoplasm forms when genes are expressed abnormally such that the natural outcome of somatic cells is associated. Mutation, deletion and amplification of genes that control cellular growth and differentiation also cause the formation of a neoplasm. Thus, it is very critical to understand the genetic components ...

Introduction

Proteins are food substances that are consumed with intention of maintaining the human hygiene and improving the status of health of living beings. However, there is a differentiated class of proteins that is referred to as the deceptive proteins. Grammatically, the term deceptive means something that is likely to create a notion that is different from the truth. In this case, the term deceptive protein is used to mean a category of protein that is intended to ensure balanced diet but instead creates a different impact in the performance of the body organs. For instance, deceptive proteins are said to create ...

The human population has had lengthy struggles trying to understand cancer. It has baffled many as it represents itself in numerous forms, and its features are exceedingly varied. Cancer requires specialist aggressive prognoses, innovative approach to its management, and early and fast response to it treatment. All these have dire economic implications to patients and their families. It is possible that a single type of cancer may behave entirely different in two patients presenting the likelihood of two types of diseases (Mukheerjee, 2010, p.1). There is no uniform theory regarding cancer that gives the common characteristics of various types of ...

1.A. Penicillins are β-lactam antibiotics in structure. The remodeling process takes place in the bacterial cell walls due to the presence of a layer called peptidoglycan layer, which includes the breakdown of β-(1,4) linked N-acetylmuramic acid and N-acetylglucosamine and cross-linking peptide chains . Cross-linking peptide chains provide rigidity to the fluid cell wall. Transpeptidase, an enzyme used in the breaking of peptide chains also reforms the peptide bonds after the restructuring of the cell wall. The penicillin inhibits the enzyme transpeptidase, which prevents the peptide bonds from reforming. Thus, penicillin makes the cell walls weaker, which results ...

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Abstract

Asthma and allergic reactions result in inflamed and swollen lung airways. Asthma is of two types, atopic and non-atopic. The asthma symptoms include excessive production of mucus, coughing, tightness in chest, wheezing, and difficulty in breathing. The triggers for atopic asthma are allergens and the hyper-responsiveness of IgE immunoglobulin. Genetic factors are involved in non-atopic asthma that relies on the propensity of developing asthma and hypersensitivity of airways. Epithelial cytokines, macrophage, eosinophils, and T cells collectively influence the fibroblast production resulting in muscle mutagenesis and bronchial ...

Isoforms of Dj-1 / Park7 Protein and Gp100

Isoforms of DJ-1 / Park7 protein and gp100 DJ-1 is simply a multifunctional protein that has a very crucial importance in oxidative stress, synucleinopathies such as Parkinson disease and cell death. Levels of DJ-1 generally decreases in cerebrospinal fluid, but they do not have a different composition in human plasma especially with patients with Parkinson illness. The total amount of DJ-1 has their attributes in the red blood cells, and they have the potential of being biomarkers of the Parkinson disease. In determining whether DJ-1 can be used as a biomarker of PD severity or PD diagnosis, we check on its abundance ...

Improving The Efficiency of Cytomegalovirus (CMV)

Abstract The Cytomegalovirus (CMV) is known to be an important pathogen among patients with prolonged confinement to the intensive care unit and longer hospital stays (Vincent, 2010). The virus is recognized to be a common form of the herpes virus, which the Centers for Disease Control described to be present in about 50% to 80% of the people in the United States who are in their 40’s (Mandal, 2014). The infection can actually affect children and adults alike, however the virus has the greatest impact among certain individuals, especially among children and those with a compromised immune system and ...

CAUSAL RELATIOSHIP BETWEEN AUTISM & MMR VACCINE

ABSTRACT This paper discusses about the causal relationship between Autism and the MMR (Mumps, Measles and Rubella) vaccine. We hypothesized that there is some relationship between the two. We observed some cases very closely with proper investigations and diagnostic tests. Total of 5876 children were selected from different parts of the United States, among these children, some were already vaccinated and some were about to get vaccinated within the time span of few days. All of these children were subjected to different physical tests, behavioral tests and diagnostic procedures. The children, who were apparently normal at the time of ...

The binding of antibodies to red blood cells can lead to potentially fatal outcomes such as hemolytic transfusion reactions, autoimmune hemolytic anemia, and hemolytic disease of the fetus/newborn.1 Information regarding the first steps of hemolytic transfusion reactions is well documented. It is, however, unclear why the severity of hemolytic transfusions experienced by different individuals ranges from mild to very severe.2 The pathophysiology of the final pathways and downstream events of red blood cell hemolysis are also yet to be fully elucidated although research is ongoing.2 A recent study by Liepkalns et al. (2012)1 has reinvigorated debate on the ...

Specific Aims

Activation-induced cytidine deaminase (AID) encodes a DNA-editing deaminase, which is a group of the Cytidine Deaminase family (Bello). This protein is concerned in somatic gene conversion, hypermutation, and class-switch recombination of Ig (immunoglobulin genes). Activation-induced cytidine deaminase (AID) has been expressed in B cells at germinal center and starts the events, which lead to class switch recombination of immunoglobulin genes and somatic hypermutation. Additionally to this primary responsibility in immune diversification, deviant targeting of Activation-induced cytidine deaminase activity leads to translocations and point mutations of oncogenes linked with B-cell lymphoma. Lately, AID has been related in active DNA demethylation (Sariah). ...

Circulating Angiogenic Cells (CACs) play a significant role in restoring, repairing and regeneration of Hereditary Hemorrhagic telangiectasia (HHT) vascular disorder. Hereditary Hemorrhagic telangiectasia (HHT) is an autosomal overriding vascular disorder. CACs are released from bone marrow into the circulation, and home to the injury site to exert their regenerative and angiogenic effects. Both clinical data and animal studies have reputable that alterations in ALK1 and ENG are dependable for the configuration of the atypical vasculature detected in the mass of patients with HHT. Idiosyncrasy of endothelial cells because of ENG or ALK1 mutations is linked, and well-matched with the vascular ...

Connective tissue growth factor induces collagen I expression in human lung fibroblasts through the Rac1/MLK3/JNK/AP-1 pathway

Many lung inflammatory diseases present pulmonary fibrosis as one of the symptoms, which is characterized by shortness of breath, chronic coughing and discomfort in the chest. It causes modifications of the lung tissues due to overgrowth, hardening and scarring. This occurs mainly due to deposition of collagen, an extracellular matrix (ECM) protein, whose turnover is regulated by fibroblast. Fibroblast is an important cell regulator that is enlisted by a site of injury. This process is driven by release of transforming growth factor-β (TGF-β), platelet-derived growth factor, interleukin-6 (IL-6), and IL-8/CXCL8. Previously, fibroblast was thought to express no or very ...

Proposal for enhancing the sensor performance or make nanowire sensor more selective and sensitive for cancer cells or for DNA.

Abstract An experiment to show how different amount of estrogen receptors requires detailed and sensitive procedures to reveal the difference between subtle in DNA binding affection. Si-NW biosensors better exposure in finding out nuclear withdrawals in cancerous cells. Extreme hypersensitive disclosure of protein-DNA synergy. A proposal in making Nanowire sensors being more selective and sensitive. Electrical implementation of Silicon-nanowire field-effect sensitive for usage in discovering cancer. Array relationship between receptors and nanowire sensors.

Introduction

[2] Cancer detection or finding cure for DNA disease have become two of the famous areas in medicine, which scientists are likely to investigate in. ...

- Cell Culture and Cell Lines: Three breast cancer cell lines: CAL120, EVSAT and MDA-MB-231, were cultured individually, in RRMI 1640 supplemented with 20% Fetal Bovine Serum (FBS), 100U/ml Penicillin, 100U/ml Streptomycin and L-Glutamine. The cells were cultured and maintained in an incubator at 370C supplied with 5% CO2. For subsequent experiments, the cells were further cultured till confluence, in 6 well and 96 well plates using RPMI+20%FBS. 1.2 Treatment of Cells: For treatment, 1x106 cells were seeded in 6 well culture plates, and incubated for 24 hrs. After 24 hours, the old media ...

Immunology Disorders

The immune system is responsible for protecting the body from various parasites, bacteria, virus etc. that try to destabilize the homeostasis. Naturally, the immune system is considered one of the most important mechanisms that help us recuperate from diseases. When the immune system malfunctions and starts destroying self-cells, it leads to certain disorders. In other circumstances, the immune system may be suppressed and fail to kill disease causing cells. Here we will discuss a few disorders of the immune system.

HYPERSENSITIVITY TYPE I

Allergy or hypersensitivity type I is also known as allergic reaction or intolerance, in this scenario the immune system creates a ...

Abstract

Organ transplantation is an important life-saving procedure for those people, who have some problems in their organs. However, the problem of organ transplant rejection is an important issue in this case. In this problem, patient’s immune system starts rejecting the organ as a foreign body. This problem of rejection of the organ can be acute or become chronic. Several diagnostic procedures have also been developed to reduce the problem of rejection of an organ. Scientists have done a lot of research in this regard. Acute rejection needs immediate care. In order to reduce the problem, specialists will check the area ...

The success of America as a country is a fact acknowledged widely by all around the globe. However, if one may ponder over the reasons behind the success of this great nation then it would mainly be accounted to two major factors: the people and the various opportunities available to them. America is known for its freedom of choice and place where dreams come true. It is highly successful nation which is famous for its ability to bring out the best in people. American laws, systems, securities and liberties are not perfect but they are the most unusual ones worldwide. They ...

Literature Review

Obesity is associated with a higher risk of cancer death, specifically deaths caused by cervical cancer. According to Wee, Phillips, & McCarthy (2005), there are about 4000 deaths every year that are caused by cervical cancer leads despite the availability of outstanding screening tests that are available in the market to detect the disease. The article of Wee, et al., (2005) is important for the study since it will explain the fact that despite early stage detection screenings that were conducted, there is no guarantee that the preventive treatment will improve the survival of patients with cervical cancer (Wee, et al., ...

The complexity of managing hyperthyroidism is largely attributable to the multi systemic effects, a diverse range of symptoms and a wide clinical spectrum ranging from subclinical to severe disease. This work briefly touches on the trends in management of hyperthyroidism and key research findings that have guided clinical approaches over the last 2 decades. Literature review highlighted some niche areas that have predominantly been the focus of research. These include, evaluation of diagnostic criteria, interpretation of laboratory results and prognostic implications of subclinical thyroid disease.

Diagnostics

Thorough clinical assessment of patients continues to be indispensible with serum TSH level being the standard ...

Meadowbank TAFE

HLTAP501A/B/C Analyze Health Information HLTCOM510B Provide Services to Clients with Chronic Disease or Conditions PATHOLOGY ASSIGNMENT –ASTHMA– Abstract Asthma is a chronic problem that influences lung function. Clinically asthma is identified by the variation in expiratory airflow and abnormal respiratory symptoms such as wheezing, chest congestion, cough, and shortness of breath. The aetiology of asthma is linked to a mixture of genetic and environmental factors, though; heredity or atopy also plays a role in asthma. Asthma is categorized into two types: extrinsic (allergic) and intrinsic (nonallergic). Allergic asthma is an outcome of the reaction of an antibody ...

Article Analysis: Ad5 Specific

Article Analysis: Ad5 Specific Since its discovery in 1983, scientists and researchers have been working hard to neutralize the threat of HIV against humans . This advance has included many strategies. Researchers have tried counterattacking the virus while it still hibernates in monkeys, once it was concluded that is where HIV originated . Other strategies have included offsetting the virus after it becomes active in humans. Unfortunately, some of these vaccinations, such as the Ad5 vaccination, allowed the vaccine to progress further, as seen in human trials . Other vectors, such as CMV were used in order to correct Ad5, and ...

Introduction

Scientific advancements have enabled antibodies that identify non-changing structures of the protein of influenza haemagglutinin (HA) to be identifiable. This development has strengthened the efforts of developing universal influenza vaccines. The goal of this research is to find out how HA stimulates naïve B cells into maturation and induces protective immunity. The researchers chose to investigate this phenomenon because the antibodies that can be used for the vaccine derive from a particular antibody gene of the region IGHV1-69. Currently, the manner in which influenza haemagglutinin stimulates naïve B cells into maturation and protective immunity remains unknown. This essay ...

Immunohistochemistry

Introduction The Human Papilloma virus (HPV) is an extremely common group of viruses that are found worldwide. There are more than 100 types of HPV worldwide. 13 types of HPV are associated with cancer. They are also termed as high risk type. (WHO, 2013). HPV is transmitted through sexual contact. More than 90% of cases are infected shortly after sexual activity. Cervical cancer is associated with HPV infection that are acquired through sexual contact. 70% of cervical cancer cases are caused by two types of HPV. HPV 16 and HPV 18 are the two strains associated with cervical ...

Introduction

Marburg virus disease (formerly known as Marburg hemorrhagic fever) is a severe zoonotic (animal-borne) disease, affecting both humans and non-primates. The source of infection for primates is unknown. However, research indicates that bats mainly infect the humans. The Ribonucleic acid (RNA) virus belongs to the filovirus family. Filoviruses belong to the order Mononegavirales. These viruses are enveloped, non-segmented, and negative-stranded. Marburg virus and Ebolavirus are the two genera of Filoviradae family. The only species in the genus Marburg virus is Lake Victoria margburg virus (Center for Food Security and Public Health, 2009, p.1). The virus has caused epidemics in ...

Prevention is crucial when it comes to combating rabies. Luckily, it is also quite easy. Prevention is classified into two methods: primary and secondary. Primary prevention: minimizing and avoiding exposure: One of the most vital steps in checking rabies is educating the endangered about sensible pet ownership as well as how they can avoid or minimize their rabies exposure. To begin with, dogs, cats and other pets must receive regular rabies vaccines. The conventional rabies vaccine was initially administered to dogs once annually, but desires to reduce vaccine frequency initiated the development of rabies vaccine that is now ...

VaccinationsName

Vaccinations: A public health controversy What is vaccination? Immunization is the point at which an infection, or microorganisms, is deliberately managed to you (for the most part by infusion) so that your resistant framework can plan to battle a future contamination. The ideal approach to do this is to open your body to murdered or debilitated infections or microbes (or parts of them), that can't result in disease. How can it function? • Most antibodies hold a smidgen of an ailment germ that is powerless or dead. Antibodies do not hold the kind of germ that makes many debilitated. A few antibodies ...

Yellow fever is a classic viral hemorrhagic fever and is an example of diseases of antiquity. It was first described in 1667 in Barbados. The name of the disease is derived from the appearance of those infected (usually a yellow tinted skin). It was earlier called as Yellow Jack because jaundice is a prominent sign in severe cases. Devastating epidemics of yellow fever were prevalent in the Americas, Europe and Africa from the seventeenth to the twentieth century. The mortality rate for yellow fever is about 20% (Narins 2003).

Cause

Yellow fever virus belongs to the genus Flavivirus (Family: Flaviviridae) and is the ...

The regeneration of nerves throughout the human body has been a subject of much interest in biology recently. The unique characteristics of both the central nervous system (CNS) and the peripheral nervous system (PNS) provide insights into the mechanisms occurring after once damage occurs. A good understanding of tThe differences between these mechanisms also may lead to new therapies for nerve-damaged patients, including stroke patients. Following injury, the nerves in the CNS of an adult mammal will not regenerate, while the PNS will. These differences appear in every stage of axon regeneration. Within hours of axotomy, the PNS begins ...

Cholera Toxin Enhances Vaccine-Induced Protection against Mycobacterium Tuberculosis Challenge in Mice

This experiment is an animal experiment made in accordance with the basic guideline the UK Animal Act 1986. Methods use was by collecting mice of 6-8wks. BCG Pasteur vaccine administered. Others vaccines are MVA85A and cholera toxin. Intradermal agents administered are the anti-IL-1A blocking antibody and the isotype IgG2a. Animals were then challenge with Biaera AeroMP aerosol nebulizer at a targeted pressure and dose. Organ harvest and stimulation for immunogenicity was then done. The lungs and spleen are the organs harvested aseptically. The organs were then processed with different steps such as mincing and digestion with DNase/collagenases. The cells collected ...

Abstract

It has been observed that cAC10 , a monoclonal antibody that is chimeric, has the capacity to cause the in vitro arrest of the growing of the CD30+ cell lines in severe combined immunodeficiency (SCID)in models of mice having Hodgkin disease (Chiarle et al. , 1999; Weiner and Adams, 2000 ). Thus, it can `be said that these antibodies have antitumor activity.

Introduction

The potency of CD30 +-targeted mAb activity was increased by generating an drug and antibody conjugate, “cAC10-vcMMAE”. MMAE is a synthetic analog of dolastatin 10. “cAC10-vcMMAE” has potent cytotoxic activity against CD30 expressing cell lines and has exhibited stability in physiological ...

Introduction

The immunological disorders in an individual can occur due to three different reasons. This includes immunological diseases in an immunocompromised individual, or “autoimmune diseases”, or finally an immunological hypersensitivity that can lead to tissue damage.

Immunodeficiency diseases

Immunodeficiency diseases occur due to malfunctioning of the organs or cells in an individual. They are further classified as primary immunodeficiency (PID) and secondary immunodeficiency (SID).

Primary immunodeficiency disease

They occur when there is a direct defect in the immune system. They are further classified into eight groups depending upon the site of the defect. Combined T and B deficiencies. Severe Combined Immunodeficiency (SCID) is an excellent ...

The human immune system is a proverbial double-edge sword. On one had humans depend on the immune system, and on the other hand there are diseases as a result of its improper function. These diseases due to its disorder in its function can range from immunodeficiency states to hypersensitivity . Put in simple terms, the disorders range from doing too little to an overreaction of the system. There disorders will be considered under the following heading: immune mechanism of tissue injury, autoimmune diseases, and immunodeficiency diseases. Before we go into the details of the diseases, we would like to describe what the ...

Abstract. The aim of the study was to validate the existence of vesicles in gram positive bacteria, bacillus subtilis. The visualization of the vesicles was carried out by various microscopy techniques. The measurement of vesicle size was performed and the amount of vesicles compared between various strains. The laboratory cultured strains was more effective in the study as they exhibited hyper production of the vesicles. The production of vesicles was also validated in environmental strain, however in much smaller amount. The vesicles were also visualized and isolated from biofilms that act as the natural environment of the bacteria. Proteomic ...

Abstract

IFITM3, a protein induced by interferon, restricts reovirus infection in host cells and inhibits the viral replication. It affects the proper penetration of the virus by altering the dynamics of the viral uncoating in the endosomes. This study is the first in characterizing the anti-viral activity of IFITM3 on non enveloped viruses that depend on endosome for cellular entry. Following contagion with a virus, the body triggers the release of a class of cytokines, interferons that are involved in first line of defense against infection. Type I interferons are specifically induced in the cells following insult with both DNA and RNA ...

Introduction

Sally Thomas is a twenty three years old mother and is comprehensively covered in this clinical scenario. The scenario covers aspects of her family history which are of essence to any medical decisions associated with any clinical procedure. The aspect of her past pregnancies will be comprehensively articulated in the case study. Her medical history will be analyzed and clinical solutions proposed for her problems. It is pertinent to note that the scenario will be comprehensive and will articulate each stage of the pregnancy. The scenario will be broken down into multiple sub section which includes the ante partum, fetal ...