Essays on Alanine

Thin Layer Chromatography of Amino Acids

Introduction The technique used for this experiment was thin layer chromatography (TLC). TLC is used to separate components of a mixture (Clark, 2016). The purpose of this experiment was to identify an unknown amino acid. The components will have different affinities to the stationary and mobile phases. The various components will travel at different rates based on their affinity to either the mobile or stationary phase (Clark). A polar stationary phase is used in TLC experiments and the mobile phase can be a combination of solvents or just one solvent (psu.edu, n.d.). TLC can also be used to monitor ...

Based on the initial symptoms, the patient most likely suffers from hypothyroidism or low thyroid hormone disorder. Further clinical tests and an examination of the family’s medical history reveals that the particular disorder could be Hashimoto’s disease; also called Hashimoto thyroiditis. Hashimoto thyroiditis is classified as an autoimmune disease. In autoimmune disease, one's immune system attacks the body’s tissue specifically the thyroid gland (Skugor & Wilder, 2006, p.2). By attacking the thyroid gland, the disease reduces the gland’s ability to produce thyroid hormones of substantial quantity. This condition leads to hypothyroidism. Thyroid hormone deficiency presents with ...

DHEA:

Dehydroepiandrosterone, or DHEA, is a hormone the human body produces via the adrenal glands, which are above the kidneys. The exact effect of DHEA is not yet fully identified; however, it is believed to be a precursor of the female and male sex hormones, namely estrogen and testosterone, among others. Precursors are substances that the body converts into hormones.

The production of DHEA usually reaches its peak around the mid-20s and declines ever after. The same applies to estrogen and testosterone, whose production also declines with age. For that reason, if an individual wants to increase their hormone levels, it ...

Introduction

Bacteria are the very first life forms in the history of our planet. Since the beginning they were exposed to extreme changes of temperature, pressure, pH and nutrients; hence, to survive, they had to adapt themselves. One strategy of survival is to go into suspension or dormancy, by converting themselves into a seed form or spores. When conditions become normal, these spores germinate back to vegetative form. This property is also seen in pathogenic bacteria that infect higher animals such as humans. One such group of bacteria is from the Genus Bacillus.When exposed to stress, these bacteria go into dormancy ...

Purpose

The experiment aims to identify two unknown amino acids using paper chromatography. To be specific, the retention factor or Rf values of standard amino acids was used to see which one matched that of the unknowns.

Introduction

Chromatography can be defined as the separation of a mixture into various fractions by distribution between a mobile phase, which can be a gas or a liquid, and a stationary phase, a liquid or solid. Different substances have different affinities for the stationary and mobile phases. A substance that has no affinity for the stationary phase will remain in the mobile phase and move rapidly through the chromatographic system. ...

Abstract

The aim of this experiment was to determine the titration curves exhibited by two amino acids (Glutamic acid and Alanine) and use them to determine their Pka values and their isolectric point. Results from the experiment reveal several interesting facts as will be seen throughout this paper. As ampoteric compounds, this paper seeks to investigate these base-acid properties found in amino acids. Therefore, this paper will have the following basic parts; introduction, procedure for titration, discussion of titration results with reference to the titration curves as well as a conclusion. The introduction part will give an in-depth look at the ...

People used to think that if they ate a certain animal, they would acquire some of the characteristics of the eaten animal. People now think that if they ingest certain chemicals that enhance the performance of a laboratory rat, they too would develop greater power. Athletes, and others, are in constant search for the latest supplement— hopefully legal— that might enhance their performance and make them better athletes. But, for most supplements, the evidence is weak, or completely lacking. Nonetheless, there are a few dietary supplements, including caffeine and creatine, that have been shown to help. A novel type of ...

INTRODUCTION

Proteins play an essential role in living cells. The basic forming units of those proteins are the amino acids which are made up of similar composition i.e. amine group, carboxyl group and a variable R-group. The laboratory tests to identify these amino acids work on identifying the variable R-groups that distinguishes each amino acid. It is very important to note that all amino acids with alpha-amino group turn purple ranging from pink to deep violet. Proline is the notable exception (only amino acids that turns bright yellow). Proline is the only one with its unique color. Other amino acids has ...

Prof:

This report describes the experiment carried out to analysis unknown mixtures using paper chromatography. The physical properties of those mixtures are taking into considerations while certain known were used as controls. The result of the analysis amino acid was found to be similar to alanine while the second spot was unknown when compared to others.

Introduction

How can mixtures be separated using paper chromatography techniques based? The factor that influences this experiment is that which considered the physical properties of the mixtures to be separated. The theoretical concept to be followed is that which depict movement of fluid in a capillary ...

Introduction

Hepatitis is a major health threat to our environment because of the associated effects on liver and the mode of transmission. Hepatitis C which is one of the types of hepatitis that has become a major challenge since the discovery. Different factors have contributed to these challenges. One of those major challenges is the inability of researchers to discover suitable vaccine for the infection. The prolonged inactive period of the causative organism in the body before clinical manifestation is another factor (Department of health). The infectious course usually leads to enlargement of the liver and this in turn affects the functions ...