Hydrolysis Reports Samples For Students

Abstract

Amylase from Bacillus Subtilis and Aspergillus oryzae were studied as representative candidates for bacterial and fungal enzymes, respectively. The activities of these two enzymes were studies comparatively over a period of 10 minutes and at temperatures of 0, 40, 60 and 95°C. The experiment was conducted to find the most effective temperatures for bacterial amylase and fungal amylase to catalyze starch hydrolysis. The data suggest that fungal enzyme was relatively more active, particularly in the first 6 minutes, than the bacterial counterpart at 0°C.
On the other hand, the fungal enzyme activity deteriorated more rapidly than that ...

Organism # 6

Molecular Biosciences 101
Section #
Purpose
Materials and Methods
Gram Staining: Three circles were marked at the bottom of a clean glass slide and they were labeled as gram(+), gram(-) and unknown respectively. The three labeled circles were then inoculated with microorganism 6 using an inoculating loop. The bacterial smears were then air dried and heat fixed over a gentle flame. The slide was then flooded with primary stain, crystal violet for a minute by placing it on a staining rack. Excess crystal violet was washed off with a gentle stream of water. Thereafter, gram iodine was added and ...

Introduction

Bacillus Subtilis are the organisms that are aerobic, Gram Positive, spore-forming belonging to the Bacillus genus. The organisms are together with other species that are closely related play a crucial role in disease, food poisoning as well as food spoilage. Lack of methods that are standardized for the identification of the organisms whether coming from the food or environment has made the tests to be difficult. Use of morphological as well as physiological tests have offered the best ways to identify organisms in the laboratory although there are inconsistent and lack of reliance in these methods.
Identification of an ...

Scientific Report

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Abstract

The experiment was performed to analyze the outcomes of temperature on the ability of fungal and bacterial amylase to breakdown starch to maltose. Therefore, the study will reveal the optimal temperature for amylase activity. The initial steps include preparing the materials and tools used during the experiment. A timetable for comparing temperature and function was generated. Test tubes and plates were properly labeled. Iodine was needed for every spot plate so that the fungal amylase, bacterial amylase, fungal amylase and the starch solution can be added to the iodine in a time and temperature dependent fashion. Observing ...

Chemistry

2. The nano carrier needs to remain in suspension in H2O, PBS, human serum, etc. What type of polymer do you need to use to decorate the surface of the nanocarrier to achieve this goal?
In order to maintain the nanocarrier in suspension in water, Phosphate-Buffered Saline (PBS), human serum a suitable solution a polymer is needed on its surface. The polymer used needs to accomplish three goals. The polymer needs to decorate the nanocarrier surface to enhance suspension and secondly, to prevent protein absorption. Another characteristic is that the polymer evades the immune system. PLGA poly(lactic-co-)glycolic ...

Purpose

Materials and Methods
Gram Stain: the test was done to differentiate Gram-positive from Gram-negative organisms. A drop of the unknown 300 was suspended on a slide and the culture spread to an oven thin film. The culture was air dried and fixed over a gentle flame. Crystal violet stain was added over the culture and allowed to stand for 60 seconds. The stain was poured off and rinsed gently. Iodine solution was added on the smear, allowed to stand for 60 seconds and the stain poured off. The slide was rinsed with running water, and a few drops of decolorizer solution ...

Identification of Codeine by Gas Chromatography – Mass Spectrometry (GC-MS)

Group Members:
Aim
The present practical exercise aims at examining a commercial Panadeine tablet for the presence of codeine and paracetamol using GC-MS.
Introduction
Methyl morphine, codeine, occurs in opium at low concentrations. Codeine is a pro-drug because it undergoes metabolism or demethylation, in vivo, to form the active analgesic morphine. The experiment 3 involves a pH dependent extraction technique. The process separates the active constituents of Panadeine. Before extracting Panadeine, however, Eosin B undergoes extraction in order to demonstrate the effect of pH on the solubility of the compound. The GC-MC allows for the separation of a mixture ...

The process of weathering deals with the breaking down of minerals, soils and rocks as well as synthetic materials as a result of contact with the atmosphere, biological entities and water. Weathering is slightly different from erosion, because it does not involve movement. Erosion involves the movement of water, wind, snow or other agents across minerals or rocks. Weathering simply takes place “in situ.” There are two significant types of weathering, chemical and physical. Each can involve a biological component, but the difference is that physical weathering has to do with soils and rocks breaking down as a result of directly contacting ...

Waste Management

Introduction
Dealing with waste has become an increasing concern nowadays, since it affects people’s life quality by impacting the health, it affects the aesthetics, it jeopardizes the ecosystems and in general, it negatively impacts the environmental sustainability. Domestic waste (generated by individuals) and commercial and industrial waste represent a quotidian aspect that authorities are aiming to handle, either through applying laws and guidelines for maintaining healthy and clean cities and villages, either by applying various technologies for actually managing the waste disposals. And the processes and technologies used to manage the various types of waste differ depending on ...

Lab Report on preparation of Esters by Esterification

Introduction
The organic esters are characterized using their pleasant and fruity odors. Some of these odors include lead to fruits such as banana, pear, and orange to have their pleasant smelling. When carboxylic acid is mixed with an alcohol in the presence of a strong acid leads to the production of an ester. The strong acid is used as a catalyst and the general reaction is as below.
The esterification reaction is a reversible reaction with the forward being referred to as esterification, while the reverse reaction hydrolysis. The general formula of an ester compound is as follows:
The experiment aimed ...

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs.
Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

INTRODUCTION

Baghouse filters are fabric filter units consisting of one or more isolated sections containing an array of fabric bags (Vatavuk, 1998). Shapes of these bags range from round, flat, tube-shaped, to pleated cartridges. In theory, gas containing high particulate matter level passes through the fabric bag surface. The particulate matter is retained while the cleaned gas is either recycled to the process or vented off the exhaust. This paper attempts to discuss the different concepts behind baghouse filters in relation to environmental engineering. Specifically, this paper discusses (a) particle pollution, (b) baghouse filter principle, (c) filter media selection, (d) types of ...

EWB Challenge 2014Plan Timor Leste

Sustainable Engineering Practice
South Australian Institute of Business and Technology
Executive Summary
This report provides details on the project that aims at facilitating for the creation of an anaerobic/biogas digester in in Codo town. This is a small town that is situated at Lautern District in East Timor, and it has a population of approximately 500 people. This town lacks a system that can allow them to dispose and manage their waste in an effective manner. They bury their waste underground, and they use burning wood to serve as a means of cooking. The report will suggest various ways that ...