Phenotype Reports Samples For Students

Molecular Diagnostics

Genomic Evaluation of Risk of Atrial Fibrillation
and Alzheimer’s Disease

Session You Attended

Abstract
The aim to of this series of experiments is to examine a patient’s DNA for association to atrial fibrillation (AF) and Alzheimer’s disease. The single nucleotide polymorphism (SNP) rs2200733, associated with an increased chance of development of atrial fibrillation. Two SNPs: rs439358 and the rs7412, located within exon4 of the human apolipoprotein E (APOE) gene are associated with increased risk of developing Alzheimer’s disease. Isolation of the DNA from patient white blood cells, followed by PCR-RFLP and cycle sequencing are among the techniques ...

B. OBJECTIVE

C. METHODOLOGY
Over a period of time of four week study three generations of flies starting with a parental (P) generation that has already separate the flies in each generation of each cross into males and females and separate the flies according to their mutations. Based on the observed numbers of each phenotype in each generation of each cross hypothesize the parental cross and use the chi-square (x2) test and if there is little difference between the observed results and the expected results, the chi-square will have a low value, thus, the hypothesis will be supported in this case.
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Lab report

Introduction.
The Mendelian genetic effort, revolve around the inheritance of biological characteristics that trail the proposed Mendelian laws brought forward by Gregor Mendel (He & University of Chicago, 46). These regulations advocate for independent assortment, trait dominance in alleles, and the segregative capability of alleles. Thus the hypothetical endeavor of this report is to ascertain the identity of the first filial generation -F1, based on the phenotypic quotients of the second filial-F2 cohorts
Fruit fly drosophila is considered ideal in genetic experiments, because they share the majority of their genes that cause diseases with humans up to 75 ...

Panther I.D.:

“The expression of ampicillin resistance and luciferase enzyme genes by E. coli transformation using the pUC18 and lux plasmids”
Abstract
The transformation process allows the uptake of DNA from the environment, with the incorporation and expression of genes. During this process, changes in the DNA of the receptor cell occur, producing some selective advantages in some cases, such as resistance to antibiotics. pUC18 and lux plasmids were used to determine the transformation process in Escherichia coli. Cells were made competent by the addition of calcium chloride and heat shock in order to induce the incorporation of the ...

Abstract

Human activities have a significant influence on marine life. Pollutants from landfills, dumps, mines, farms, etc. can pollute the ocean and later through the food chain cause harm to all land animals and humans as well. Protein is a very important structural component of an organism. Using protein for energy is an inefficient form of metabolism and in the long run detrimental to the organism. A significant low level of protein content was noticed in Mytilus californianus or (mussels) obtained from the polluted environment when compared to the tissue of Mytilus californianus obtained from pristine environment. Organism that are ...

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Abstract

Polymerase Chain Reaction is a useful technique for the detection of genes done through the amplification of specific sites. Alu elements are a polymorphic group of elements found in the primate genome amplified by retroposition. The Alu element help in the polymorphism of the populations. The aim of this study was to use the PCR technique to detect the Alu insertion TPA-25 in cells from student population and its relation with genotype frequency, allelic and ability to establish the Hardy-Weinberg equilibrium. Samples were taken from 81 students. Cells were obtained by mouthwash and Polymerase Chain reaction ...

*Corresponding author. email:

Abstract

Alu elements are a group of mobile elements amplified in primate genome through retroposition. One of the Alu elements is the TPA-25. This element presents a high degree of dimorphism within human population, showing different alleles frequencies in different human group. Polymerase Chain Reaction (PCR) technique allow the amplification of part of the nucleic acid, including DNA being detecting by agarose gel. The objective of this study was to compare the genotypes of several individuals by using the Alu insertion detected by the Polymerase Chain Reaction. Cell samples from 82 students were taken. Samples proceed from ...

Abstract

The objective of the current experiment is to describe how plants function and pass on the traits. Dark is dominant to wrinkled and Green is dominant to pale green. A statistical approach is used for goodness of fit between observed data and data that are predicted from a hypothesis. Null Hypothesis: The results of the experiment are in agreement with the Mendel’s Laws. Dark is dominant to wrinkled and Green is dominant to pale green. The chi square values that are greater than 3.841 are expected to occur less than 5 percent of the time due to random sampling ...

Introduction

The article is a research report on the social economical effects on early childhood development. The grounding aim of the research was to uncover the truth behind the subjectivity of children at early age to physical health problems as a result of poverty. The researchers in their aim to understand these findings utilized the conceptual framework relationship between biomedical and behavioral. The article seeks to make an outline of this framework and make prior discussions about its legitimacy (Chen, 2013).
The article discards the long common perception of associating certain diseases and disorders to old age. Heart diseases and certain ...

Lab partners

The transformation of bacterial cells using Puc18 plasmid as a vector
Abstract
In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...