Chromatography Reports Samples For Students

Analysis of Plant Pigments with Liquid Chromatography and Spectrometric Detection

The experiment is aimed at coming up with a solution of knowing on how to separate color pigments in a mixture of some solvent. An appropriate solvent is put into use and then some conclusion is drawn from the observations made. The following could be the

- The extract becomes darker progressively due to the fact that the spinach solution becomes more concentrated with the subsequent extractions of the acetone solution. It is observed that the extracts starts at around 10ml of the organic solvent, but as the experiment proceeds, the volume becomes 2.0ml which was almost as the same volume ...

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs.
Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

Identification of Codeine by Gas Chromatography – Mass Spectrometry (GC-MS)

Group Members:
Aim
The present practical exercise aims at examining a commercial Panadeine tablet for the presence of codeine and paracetamol using GC-MS.
Introduction
Methyl morphine, codeine, occurs in opium at low concentrations. Codeine is a pro-drug because it undergoes metabolism or demethylation, in vivo, to form the active analgesic morphine. The experiment 3 involves a pH dependent extraction technique. The process separates the active constituents of Panadeine. Before extracting Panadeine, however, Eosin B undergoes extraction in order to demonstrate the effect of pH on the solubility of the compound. The GC-MC allows for the separation of a mixture ...

Purpose

The experiment aims to identify two unknown amino acids using paper chromatography. To be specific, the retention factor or Rf values of standard amino acids was used to see which one matched that of the unknowns.

Introduction

Chromatography can be defined as the separation of a mixture into various fractions by distribution between a mobile phase, which can be a gas or a liquid, and a stationary phase, a liquid or solid. Different substances have different affinities for the stationary and mobile phases. A substance that has no affinity for the stationary phase will remain in the mobile phase and move rapidly through the chromatographic system. ...

Introduction

Chromatography is a term that refers to the separation of chemicals in a mixture so that they may be used or detected separately. Gas chromatography involves separation of compounds with comparatively low boiling points for easy detection in complex mixtures. The separation compound is based on their variations in vapor pressures and level of affinity to solid material at the test instrument. Since the vapor pressure of each compound depends on the intermolecular forces pulling molecules together, gas chromatography utilizes any of the properties of matter. In this technique, a sample of test chemical is injected through a syringe into a specialized instrument. A ...

Abstract

Instance of countries introducing restrictions on the amount of harmful gases released to the atmosphere is on the rise. Countries like the United States have introduced policies which govern motor vehicle pollution. Gasoline is the main component of debate. As a result several studies have been done to determine the components of gasoline. In this experiment, components of gasoline are determine by an analysis process called gas chromatogram.
Samples of gasoline used in the experiment are collected from four stations BP, Marathon, Moto-Mart and Sunoco from Ashland. The samples are then analyzed using a gas chromatogram. 10 ml was used ...

Dihydrofolatereductase (DHFR) is an important enzyme in the formation of DNA (Goodsell, 2002). DHFR controls the amount of tetrahydrofolate in the cells, which in turn is an important cofactor for purine and thymidylate synthesis and ultimately in the growth and proliferation of the cell (Davidson &Sittman, 1999). The enzyme is common to many species on earth. Human and bacterial DHFR have a relatively low rate of primary sequence agreement at 26%. However, they both retain a common general structure.The key role that the enzyme plays in biology as well as the variations that the enzyme displays makes it an important target ...

Introduction to Use of Silica in Sampling of Plant Compounds

Liquid chromatography is the most widely accepted and fundamental methodology for the separation and sampling of plant compounds. Liquid chromatography has the abilities to separate and sample a huge variety and diverse range of organic compounds ranging from proteins and peptides to small molecule protein drugs. The liquid chromatography is a technique that works by placing or injecting a minute volume of liquid sample into a silicon tube which is packed with porous substances or particles. This phase is called the stationary phase. The next phase is the extraction phase where the individual compounds present in the sample are transported over a packed tube ...

- Purpose
Paper chromatography is a technique which is used to distinguish between the various components of a mixture. It helps to separate out the components of a solution and identify individual components. In Paper chromatography, filter paper is used as the primary material for conducting the experiment.
- Procedure
For the experiment, a beaker of 1L capacity is taken and filled with 100mL solvent. The beaker is known as chromatogram tank. Next, a filter paper around 12 inches long and 9 inches wide is taken. A light line which is 2cm apart from the bottom of the paper is drawn ...

Aims and Objectives of the Experiment

The aim of this experiment is to study the separation of an extract of a non-prescription analgesic tablet containing acetylsalicylic acid (aspirin) and caffeine. The separation is achieved through a well-known process called liquid chromatography. The quantities of the aforementioned substances also need to be determined in the experiment (“High Performance Liquid Chromatography,” n.d.).

Theory and Principles

Chromatography is a technique used for the separation of the components, or solutes, in a mixture. The separation is based on the amounts of the relevant solute in the moving, or fluid, stream. There are two phases in chromatography, the mobile phase, and the stationary ...

Prof:

Introduction
This is just one of the simple ways of identifying unknown compounds and separate mixtures. It is a separation technique that works based on the compound interactions as those compounds tend to move within a supporting medium. The compounds interact in two phases which are the mobile and stationary phase. The technique helps in analyzing, identifying, purifying and quantifying unknown separable mixtures. The mobile phase is either a liquid or gas which moves the solvent through the stationary phase during the process. The stationary phase is a liquid or solid component that is fixed in a place for the procedure.
Paper ...

The experiment involved using the simple paper chromatography technique in the separation of a black marker ink as described in the science project lab’s website (Science Project Lab, 2014).

Materials Used

Coffee filter
1-liter glass jar
Paperclip.
Pencil
Water soluble black marker
Scissors.
Vinegar and glass cleaner
Procedure
The chromatography strip was obtained from coffee filter and cut into a peace that can fit into the jar. A line was drawn one inch from the bottom of the strip. A line was drawn using the black marker along the marked line. The solvent (glass ...

Introduction

Steroids play a central role in enhancing human growth, development and normal health, since they take part in development of secondary sexual traits, reproduction, gene expression, neurological and cardiovascular functions and maturation among others. Accordingly, imbalances in the metabolic mechanisms of steroids in humans is associated with an array of health complications such as cardiovascular and neurodegenerative diseases and cancer among others. The foregoing underscores the importance of steroid profiling to establish its levels in humans, particularly in disease diagnosis, because it enables health professionals to understand a variety of steroid-related health conditions. For efficiency and accuracy, a good profiling method is ...

Dr. Professor

Paracetamol is crystalline white power with the scientific name N-(4- Hydroxyphenyl) acetamide. The chemical formula is C8H9NO2 and its structure is

Properties

The melting range of paracetamol is 168 to 172oC and its solubility is 14.3g / dm3 of water and ethanol’s 100g / dm3. It is insoluble in ether and dissolves quickly in chloroform. It dissipates uv absorption minimum in aqueous acidic medium at 245 nm and has uv absorption maximum in aqueous alkaline medium.

Assay

Through titration, paracetamol is analyzed using ceric ammonium sulphate. In the titration, Ferroin plays the role of indicator. It is also assayed ...

INTRODUCTION

Proteins play an essential role in living cells. The basic forming units of those proteins are the amino acids which are made up of similar composition i.e. amine group, carboxyl group and a variable R-group. The laboratory tests to identify these amino acids work on identifying the variable R-groups that distinguishes each amino acid. It is very important to note that all amino acids with alpha-amino group turn purple ranging from pink to deep violet. Proline is the notable exception (only amino acids that turns bright yellow). Proline is the only one with its unique color. Other amino acids has ...

Question 1: Separation of mixtures

1. Extraction

This is a method of separation of two immiscible solvent phases. It is the best method for the separation of a mixture of Cyclohexanone and Acetone (miscible)

Reason:

Cyclohexanone is insoluble in water while Acetone is miscible with water. A mixture of the two compounds forms two distinct layers in water. Distillation cannot be used because the two compounds are highly flammable.

Procedure:

In this method of separation, water is added to the mixture. Acetone completely mixes with water while cyclohexanone does not. A funnel is then used to separate the two.

(Note: the colouration is ...

Executive summary 3

Introduction 3
How the company handled the challenge 4
Research findings and future of the company 6
Conclusion 6

Bibliography 7

Executive summary
A product’s life cycle and its source industry are characterized by maturity, growth and also the eventual decline. Better and new technologies besides changing the tastes of a consumer exhibit the speed of decline of an incumbent, commoditization, and finally death. However, the humble Corticeira Amorim company has remained strongly from this terrible norm, portraying a fascinating situation of the survival of an industry and dominance for long. Cork owned the monopoly and it has ...

Abstract

This experiment sort to find an accurate and most viable method that would be used in laboratories to the measurement of iron concentration in aqueous samples. The methodologies that were identified for further analysis included; colorimetric assay (molecular spectroscopy), flame atomic absorption spectroscopy, redox titration (volumetric) and cation exchange chromatography (EMA technique). These methods were analyzed of their advantages and later subjected to a validation test. In the process, a wide range of CRMs were used alongside six other samples that contained unknown iron concentration.

The experiment was thus, geared towards providing answers to statements like; the necessity of quantification of ...

Introduction

Reduction reactions are very important in chemistry as they are effective processes for synthesizing new compounds from existing ones. In organic chemistry, a compound is said to be reduced if it acquires new hydrogen. There are diverse reagents for carrying our reduction reactions. Two of these are sodium borohydride (NaBH4) and lithium aluminum hydride (LiAlH4) - the latter is a more powerful reducing agent compared to the former.
In this experiment, NaBH4 was used to reduce p-Nitrobenzaldehyde to form p-Nitrobenzyl alcohol. NaBH4was used over LiAlH4 in this experiment, because LiAlH4 would also reduce the nitro group of the aldehyde.

Experimental:

...

Executive Summary

The beverage industry as a whole is changing in rapid fashion. More middle aged adults are becoming increasingly concerned with nutrition, and this includes a considerable portion of the baby boomer population in the United States and elsewhere. Many are becoming aware of the fact that beverage choices today do impact overall lifestyle and possibly life expectancy on the whole. As such, the non-alcoholic beverage sector continues to be impacted by this reality as the public opts for healthier drinks. Coca Cola continues to respond to this need. Rather than live in denial, the company has been actively working ...

Abstract

The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...

Osmosis is the random diffusion of solvent water molecule from an area of low solute concentration to areas where the solute concentration is high through a semi permeable membrane so as to equalize the concentration of the solute on both sides (Kylstra, Longmuir, and Grace 289). It is a biological process essential to both flora and fauna that results into various physiological effects, for this experiment water and a mixture of syrup and water will be used to demonstrate the process. A laboratory report hence illustrates what the students involved, understood while carrying out the practical experiments.
The purpose of the ...

Abstract

In this experiment, 5 mL of toluene solution was transferred in a 25 mL round bottom flask and then 0.40 g maleic anhydride and 0.4 g E, E-2, 4-hexadien-1-l added. Ethyl acetate was used as a solvent to carry out a TLC analysis on the mixture produced after reaction completion mainly to determine the presence of any starting material in the mixture. The reaction mixture was then slowly cooled to room temperature. There was crystallization taking place as the reaction cooled, white crystals were formed.
The mixture was further cooled in for 10 minutes in a water bath to promote crystallization to ...