Essays on Active Site

Introduction

Enzyme activity is affected and influenced by several factors amongst which include temperature, pH level among others. High temperatures, for instance, denatures the enzymes hence inhibiting their activity, low temperature, on the other hand, inactivates the enzymes making them unable to digest the food. Enzymes are catalysts which mean that they mediate the digestion of food and their assimilation into the blood streams. They oversee the conversion of the substrate into products without being destroyed or changed in any way. (Cohen, pp134) The enzymes have a primary and tertiary structure. The tertiary structures of an enzyme are important in ...

Introduction

Capsular polysaccharides are of high molecular mass and low molecular weight cell-surface polysaccharides of Gram-positive and Gram-negative bacteria (Wang et al. 2000). They facilitate evading the immune system; therefore, the production and extraction of this layer is a prospective therapeutic target (rcsb.org). In the Gram-positive bacterium Streptococcus pneumoniae, strain TIGR4 the Cps4B is tyrosine protein phosphatase CpsB. The phosphatases have capability to dephosphorylate their analogous autokinases, instead of their similar functionality equivalence; they don’t demonstrate sequence homology. This capsule or CPS act as an interface border between a microbe and its environment that presents an expansive biological and clinical significance. ...

Introduction

Enzyme refers to a molecule that is protein in nature and functions as a catalyst to speed up the rate of a given reaction. The understanding of the basic theory of enzyme kinetic is an essential in the understanding of the mechanisms involved in the activity of an enzyme, as well as in selecting the best method of analyzing the enzyme. There are several factors that have an effect on the rate of an enzymatic reaction. These factors include temperature, pH, enzyme concentration, as well as the concentration of the substrate (Worthington Biochemical Corporation, 2013).

The increase or decrease of the level ...

1. When placed on an agar slab and incubated at 30°C for 2 days, a single yeast cell proliferates into a colony with a diameter of 2 mm containing 108 yeast cells. If the cell has a functional Ade2 gene (Ade2+), the colony will be white; if Ade2 is non-functional (Ade2–), the colony will be red. You take a strain with a mutant Ade2– at the regular chromosomal position (red colonies) and insert a second, functional Ade2+ gene near the telomere. You find that the colonies are a mixture of red and white patches or sectors. Figure 1 shows some colonies; the black regions represent the ...

Living organisms depend on biochemistry to power various chemical reaction cycles which are important for the overall biological functioning of an organism. Chemical reactions occur at a specific rate in certain conditions and require input energy (activation energy) to be supplied before a reaction can begin. Since a reaction cycle consist of many consecutive step, slowing one step slows down the whole cycle. Enzymes are proteins which act as catalysts in biochemical reactions and speeds up reactions to rates that are high enough for biological functioning. Enzymes manage this by providing an alternative reaction route with lower activation energy.

The breakdown of ...

Report on Case 1: Diagnosis of Hereditary Fructose Intolerance

Enzymes act as biological catalysts, they speed up biological reactions and are also capable of regulation. Enzymatic reactions can be anabolic – where a new substance is built from smaller ones; or catabolic – where a substance is broken down to yield simpler molecules. In the example of fructose, enzymes catabolize this simple sugar to substances that are intermediates of the glycolytic pathway, allowing fructose, a 6-carbon sugar, to be used by cells to obtain energy in a way similar to glucose (also a 6-varbon sugar). Aldolase B is one of the enzymes involved in the catabolism of fructose, and its deficiency results in ...

Background

Early scientists and nutritionists believed that the most vital nutrient in living organisms, for building and maintaining the body was the protein. The role of proteins as enzymes came into existence several years later. This was around 1926 when experiments showed that enzymes were proteins. Insulin was the first protein to be sequenced. Hemoglobin was the first structure of proteins to be resolved. Myoglobin was then resolved later.

X-ray diffraction analysis (X-ray crystallography) led to the determination of the structure of the proteins (three-dimensional structure). The purification of proteins can be made possible from cellular compounds. Several methods have ...

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs. Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

Aim

The experiment aims to study the effect of substrate concentration on the activity of polyphenol oxidase (PPO) from banana extracts using spectrophotometric techniques.

Introduction

An enzyme refers to a molecule that is protein in nature and works as a biological catalyst. The enzyme possesses three major characteristics. The first characteristic is that they act to increase the rate at which a given reaction takes place. This enables most cellular reactions to take place at up to million times faster compared to their rate in the absence of an enzyme. Secondly, most enzymes act specifically on a single reactant known as a substrate to produce the ...

Abstract

Enzymes are the large biological molecules that play the role of metabolic processes responsible for maintaining the human life. They have the ability of high selectivity, as natural catalysts. They accelerate both the specifity and rate of metabolic processes, from food digestion and DNA synthesis. Most scientific enzymes are proteins in nature, even though some RNA molecules which are catalytic have been studied. The structures of enzymes normally adopt a three dimensional structure, and in order to assists in catalysis, it may employ the organic and inorganic forms. This experiment is meant to describe the properties and characteristics of enzymes ...

Part A

How Enzymes Act As Protein Catalysts in Biochemical Processes Catalysis refers to the speeding up of a chemical reaction by a catalyst. A catalyst is a substance that accelerates a chemical reaction but does not undergo a permanent chemical change in the process. Enzymes are catalysts whose building blocks are amino acids: they are proteins. Enzymes also exhibit a defined three-dimensional structure. In addition, they have a cleft on their surface called active site. The active site is the region where substrate interactions take place. Enzymes play important roles in fructose metabolism. The first step of fructose metabolism in the liver is the phosphorylation of ...

- Why are mutations at D185, D186 and D110 are so detrimental to RT activity? (15 Points) Mutations at D185, D186 and D110 are very detrimental to RT activity owing to the fact that the three carboxylate residues have been documented to participate in some forms of physiological processes and metal coordination function that is fundamentally required in the binding of deoxyrobonucleotide substrates to the enzyme. And so, when these subunits are interfered as a result of mutation then the fore mentioned biological process are essentially incapacitated. Furthermore, the region was also subcloned due to mutagenesis hence the high ...

1.0 Introduction Websites are arrangements of interrelated web pages parented by a single web address or better known as a domain name hosted by a server. Initially websites were typically navigated for information only. The Internet evolved to incorporate unlimited functions such as electronic email communications, architectural designs and business transactions. Nowadays most, if not all, websites are designed to be a two-way method of communication between the sender and the receiver. The purpose of this report is to evaluate three (3) different kinds of websites and distinguishing their main functions: information, interactive, and trans-active. Considering how virtually most established and popular ...

Introduction

Enzymes are proteins which have the ability to facilitate metabolic reactions without being used up in the reaction. The enzyme amylase is responsible for facilitating the hydrolysis of starch to form sugars that are then assimilated by the body. Amylase is an enzyme needed for the digestion of carbohydrates for nutritional purposes. Starch is one of the most important sources of energy in the body. Starch is a polymer made up of several monosaccharide and disaccharide monomers. However, starch cannot be absorbed and used in the body as it is (Cornish-Bowden, 134). Consequently, it has to be digested into smaller ...

Introduction

Components that enhance the rate at which chemical reactions that place are referred to as enzyme (Lehninger, Nelson, & Cox, 2008). Through the reduction of the amount of activation energy that is necessary for a successful completion of a reaction, enzymes manage to reduce the time and resources needed to complete the reaction (Cooper, 2000). The activity of a given enzyme is, usually, affected by the level of different parameters such as temperature, pH levels, concentration of the enzyme and substrate concentration (Eed, 2012). Enzymes work best within a given range of values for each of these factors and deviation from these values ...

Introduction

Biological reactions are those reactions that are involved in various activities, in the body including energy production. Reactions that produce energy are called exergonic reactions while those that use energy are called endergonic reactions. Enzymes enable chemical reactions to take place within a living system most of which are under a homeostatic constraint. In these reactions, enzymes function as a catalyst by lowering the activation energy necessary in a reaction (Cooper, 2000). Enzymes bring the reactants involved in a reaction closer together weakening the chemical bonds and this enable the reactions to occur faster compared to reactions that have no enzyme (Farabee, 2010). ...

Question 1

The cerebellum is an essential part of the neuromuscular system in that it plays major roles in the timing of muscular activities and the smooth progression of one muscular movement to the next. The summary of how this is done is that it sequences all the muscular activities necessary for a particular movement, and then makes corrective adjustments as necessary when the movement is being executed so that they conform to the motor impulses being generated by the cerebral cortex and other significant parts of the central nervous system (Ganong, 2005). For instance, for an individual holding a cup and ...

Introduction

The activities that take place in a living organism are mainly controlled through the use of enzymes. Enzyme refers to a molecule that is protein in nature and functions as a catalyst to speed up the rate of a given reaction. An enzyme as a protein molecule has three main characteristics. First, an enzyme has its basic function is as to increase the reaction rate and this makes most of the cellular reactions occur more than million times that they would have if the enzyme was absent. Second, almost all enzymes act in a specific manner with only one substrate in order to produce the ...

Introduction

An enzyme is a protein with catalytic properties in biological systems. During an enzymatic reaction, a substance, called the substrate, binds to the active site of the enzyme and is then turned into one or more substance(s), called the product(s). The enzyme itself is not consumed or altered during the reaction. The enzyme activity depends on factors, including pH, temperature, buffer composition, enzyme concentration, substrate concentration, and the presence of inhibitors. Enzymes have an optimal pH at which their activity is maximal. pH determines the ionization states of certain amino acids at the active site that are critical ...

Abstract

Amylase from Bacillus Subtilis and Aspergillus oryzae were studied as representative candidates for bacterial and fungal enzymes, respectively. The activities of these two enzymes were studies comparatively over a period of 10 minutes and at temperatures of 0, 40, 60 and 95°C. The experiment was conducted to find the most effective temperatures for bacterial amylase and fungal amylase to catalyze starch hydrolysis. The data suggest that fungal enzyme was relatively more active, particularly in the first 6 minutes, than the bacterial counterpart at 0°C. On the other hand, the fungal enzyme activity deteriorated more rapidly than that ...

Question 1

Photosynthesis is the process by which green plants manufacture their own food using carbon IV oxide and water in the presence of sunlight. On the other hand, respiration refers to the process by which by which glucose is oxidised is the presence of enzymes to release energy. The process by which human beings obtain energy from the sun is linked to both respiration and photosynthesis. Green plants manufacture their own food by use of carbon IV oxide and water in the presence of sunlight. Human beings obtain the energy through feeding in food from the green plants. The plants ...

Aim: The aim is to determine the effect of temperature on the rate in which pepsin digests albumen.

Research Question: What is the effect of temperature on the rate in which the enzyme pepsin digests protein (albumen)? Background Information: Enzymes are globular proteins acting as catalysts of chemical reactions. An enzyme has an active site: region on the surface of an enzyme catalyzing a chemical reaction involving the substrates and to which substrates bind. The active site of an enzyme has a unique shape; therefore, the enzymes can only catalyze certain reactions since solely substrates of the same size can fit. The enzyme is therefore specific to its substrate; consequently, the enzyme is seen as ...

Enzymes are sometimes referred to as biochemistry workhorses because they are utilized by many scientists to speed up a number of chemical reactions. Enzymes are proteins that speed up, or in other words catalyze, specific biological and chemical reactions by increasing the rates of reaction by more than a million factors. An example of these enzymes is Pectinase, which is the enzyme that catalyzes pectin breakdown. Pectin is a part of the cell wall of fruits like tomatoes, mangoes, oranges and apples. Pectins are large colloidal molecules and they are responsible for holding or determining the amount of dispersed particles in extracted ...