Introduction
Cloning is a term used to describe different methods of duplicating biological materials. Plasmids can be used to ‘clone in a gene’ and then express a given protein in mammalian cells. The type of protein used here is called AWP1 protein which is associated with PRK1. This type of protein is a universally expressed protein and the AWP1 gene is switched on during the early development of a being. The AWP1 may play a regulatory role in the mammalian signal transduction pathways. The AWP1 posses a conserved zf-A20 zinc finger domain at its C-terminal. When it is ...