The type of enzyme protease is serine protease as the enzyme shows maximum activity at neutral pH (7.5). The activity of the enzyme showed maximum fluroscence at pH of 7 and the temperature of 25 degrees Celsius. Serine protease enzymes are active at neutral pH. Also, the activity of the enzyme was reduced at alkaline pH of 8.5 and also at higher temperatures of 42 degrees Celsius.
Yes, enzyme protease exhibits Michaelis-Menten kinetics. The graph represents rectangular hyperbola closely and therefore exhibits Michaelis-Menten kinetics.
y = 25.492x + 0.472R² = 0.9025
Vmax= 1/0.472= 2.12 micromole/minute
Km=25.492/2.12 =12.02 micromole
y = 11.999x + Continue reading...