Essays on Nucleotide

Introduction

Exom sequencing is also known as whole exom sequencing (WES). This sequencing technique is used to sequence all the expressed genes in a genome also called as exom. It is one of the most widely used targeted sequencing methods. The exom which is the protein coding region of the human genome consists of less than 2% of the genetic code. This region is responsible for containing more than 85% of known disease-related variants. Thus, whole exom sequencing is a cost-effective alternative to whole genome sequencing (WGS). This technique is efficient in identifying coding variants across a wide range of ...

Introduction

Atherosclerotic cardiovascular disease (CVD) involves peripheral circulation, the brain, and the heart. The disease is a primary health issue in many countries around the world (Sawhney, et al., 2012). CVD develops through complex processes involving novel risk markers and common risk factors. Decades of research have demonstrated that a sibling’s or parent’s family history is linked to the development of atherosclerotic CVD (Gupta et al., 2013; Nelson, 2013; Imes and Lewis, 2014), which manifests as peripheral arterial disorder, stroke, and coronary heart disorder (CHD). Various Mendelian disorders have also been shown to contribute to atherosclerotic CVD. Although ...

Introduction

Cancer is a disease that is associated with high morbidity and mortality rates. The Prognosis of the diseases in many of the patients is also poor (Adorno & Brownell, 2014). The Economic cost involved in treating the conditions and other factors serve as an incentive for studies into approaches for the identification of high risk people. The development of brain metastases in patients diagnosed with lung cancer further amplifies the disease burden (Adorno & Brownell, 2014). This paper summarizes two articles in which the authors present content on the use markers in the identification of high risk populations.

Summary of Articles

The article by ...

Vid30 complex participation in the degradation of CDC25 in yeast and their role in Ras/ cAMP/ PKA pathway

Background: Unicellular organisms such as Saccharomyces cerevisiae in its total life cycle is exposed to diverse and changing nutrient conditions. Thus, in order to survive and achieve a competitive advantage, it has adapted itself such that it not only uses the wide range of available nutrient sources but also the sources that are nutrient rich. It mainly uses fructose and glucose by rapidly fermenting to alcohol. In response to these nutrient conditions, the intracellular trafficking transcription and turnover of protein from nutrient transporters (e.g. Hxts) get regulated. Therefore, when the availability of the glucose increases, the expression of low- ...

{Author Name [first-name middle-name-initials last-name]} {Institution Affiliation [name of Author’s institute]}

Introduction

Polymerase Chain Reaction (PCR) is a very influential technique that helps in amplifying the specific segments of DNA. This biochemical procedure is performed in-vitro that is quite different from cloning. This technique is a fusion of synthesis protocol of distinct repeated segments of DNA and thermostable DNA polymerase application that has given scientists a very potent analytical, immunological and molecular technique used prevalently in clinical laboratory science (Garibyan & Avashia, 2013). PCR is based on three primary steps that include denaturation of the template into single strands, annealing ...

1. DNA in forensic science: DNA contains the genetic code of a person. The genetic information stored in the DNA, is coded in the form of nucleotide sequence. The nucleotide sequence is formed by four fundamental nucleotides: A, T, G, C nucleotide, that formed a polymer in a set order. A typical human DNA, has 3 billion nucleotide bases. The DNA sequence is 99.8% identical in all human beings and is only 0.2% dissimilar. This dissimilarity is the basis for identification of the suspects in forensic medicine. No two human beings can have a DNA that is 100% identical. ...

INTRODUCTION

Obesity is a disorder that increases body fat and predisposes an individual to many health complications and problems like diabetes, heart diseases and high blood pressure . It is acquired, when the consumption of calories over time exceeds their burning. Factors that may influence the occurrence of obesity are lack of physical activity, genetic profile, overeating, consumption of calorie-rich foods. An individual is considered obese, when his weight is at least 20 % more than it should ideally be and has a body mass index (BMI) is over 30. According to the survey conducted by

National Health and Nutrition in 2009 – 2010, over 35 % adults were regarded as obese .

FACTORS CAUSING OBESITY Consumption of excessive ...

Biology

(Department)

DNA and its importance in our lives

Introduction Deoxyribonucleic acid (DNA) is a long linear polymer located in the nucleus of a cell. It is developed from nucleotides and shaped like a double helix. It is related to the transmission of hereditary information from one generation to the second generation. DNA contains the information for the development as well as reproduction of an organism, thereby leading to a normal life. In the year 1953, James Watson, a biologist, and Francis Crick, a physicist, discovered the structure of DNA in a laboratory in England (LEROY, 2003). The structure of DNA helped scientists to know the process ...

Bio Exam

Codominant traits exist when two different alleles for a genetic trait such as flower colour, and hair texture are both expressed. The expression of codominance infers a relationship between two types of a gene. In codominance, the phenotypes of both alleles inherited from the parents are expressed ("Codominance - Glossary Entry", 2016).

Genetic expression feather colors in purple penguin ("Basic Principles of Genetics: Probability of Inheritance", 2016).

With the order of dominance as pd>pm>pl>pvl a 1 dark: 1 medium ration of phenotype among the baby penguins is expected. Marfan Syndrome is a genetic disorder that exhibits variation among affected individuals. The range of phenotypic severities is an example of variable expressivity. ...

Epigenetics is the process of studying cellular and physiological phenotypic characters within the field of genetics. The study falls within the subject of genetics and the phenotypic differences which are affected by the peripheral environmental matters. These factors are notorious for switching genes on and off and also affect the way in which genes are read by the cells. Therefore, Epigenetics is the study that seeks to establish the dynamics alterations in the transcriptional potential of the cell. The alternations in this case, may or may not be heritable, even though it is controversial to use the term epigenetics ...

Gene Editing Techniques

1. Gene Editing – General Background Gene editing entails inserting, deleting and replace the DNA in a genome of an organism utilizing engineered nucleases. Sections of the natural gene are replaced and completed using a synthetic DNA chain. Also, the natural repair process gets rid of the mismatches and gaps in the DNA. On the other hand, gene disruption is a technique by which the DNA fragment is utilized to replace a genome sequence with a chosen marker gene like the kanavanine resistance. DNA –binding proteins are proteins that consist of DNA-binding domains and hence have affinity for single and ...

Hepatitis B virus do not need presentation - its statistics tell about it more as there are more than two hundred million infected people in the world (Sundaram and Kowdley, 2015). In many patients hepatitis B infection leads to the development of the liver cirrhosis and other complications, can cause decompensation of the liver function and lead to death. Prevalence and malignant course of this infection make it a major challenge for modern medicine. Reviewed article lists and shows clinically proved and effective methods of the hepatitis B management. The discovery of a vaccine became the turning point in ...

The process of DNA replication at biochemical level

DNA or deoxyribonucleic acid is a biopolymer that encodes all the structures needed for the organism’s lifespan and development. In a cell it is located in the nucleus, mitochondria and chloroplasts. DNA consists of four types of deoxyribonucleotides, the sequence of which is used to store the data.

The essential characteristic of DNA molecule that allows it to pass the stored information to the next generations of cells or organisms is the way of self-reproduction known as replication. The molecule consists of two complementary chains that are held together with the help of hydrogen bonds between the complementary bases. ...

Introduction

Prostate cancer was identified in 1853three hundred years after the discovery of the prostate gland by Andreas Vesalius in 1538. Prostate cancer was considered as a rare malady, as a result, of the poor detection methods in the 19th century. The treatment of the rare malady was the removal of the testes in the 1890s, in the dawn of the 20th century doctors shifted from the removal of testicles to the removal of the prostate gland. Charles Huggins discovered chemical castration, a procedure that involved the use of estrogen to reduce the influence of testosterone in men who were suffering from prostate ...

1. When placed on an agar slab and incubated at 30°C for 2 days, a single yeast cell proliferates into a colony with a diameter of 2 mm containing 108 yeast cells. If the cell has a functional Ade2 gene (Ade2+), the colony will be white; if Ade2 is non-functional (Ade2–), the colony will be red. You take a strain with a mutant Ade2– at the regular chromosomal position (red colonies) and insert a second, functional Ade2+ gene near the telomere. You find that the colonies are a mixture of red and white patches or sectors. Figure 1 shows some colonies; the black regions represent the ...

Acquired immunodeficiency syndrome (AIDS) is a condition caused by a progressed state of infection by the human immunodeficiency virus (HIV). The retrovirus can be transmitted through bodily fluids such as blood, semen, breast milk, and vaginal fluids via the bloodstream or mucous membranes (Moss; Maartens et al.; Simon et al.). Infection by HIV typically has four stages. The first stage is acute or primary infection which may last up to a few weeks, during which the infected person may either be asymptomatic or have flu-like symptoms such as fever, rash, malaise, body aches, chills, and swollen lymph nodes. Progression of the ...

Biology of Disease

Mode of action of;- Dexamethasone The Glucocorticoid receptors are discovered intracellularly in all tissues. They enter cells through inactive dispersion and structure a complex with a receptor protein (Rhodes & Hartnoll, 2002, P.10). This intricate then experiences an irreversible enactment and enters the cell core, where it ties to the DNA, hence prompting organic impacts instigated by these hormones, which include expanded hepatic gluconeogenesis, as well as expanded lipolysis (Ahmed, 2007, P.13). Dexamethasone is utilized to treat numerous provocative and immune system conditions, for example, rheumatoid joint pain and bronchospasm. Idiopathic thrombocytopenic purpura, an abatement in quantities of ...

Abstract

A polymerase chain reaction also known as PCR refers to a technique that is used in the amplification of a DNA fragment making huge numbers of the original fragment. The process uses an enzyme known as DNA polymerase to multiply the DNA fragment. Other components that are necessary for a PCR reaction are the DNA template that is provided by the sample being multiplied, the primers, and nucleotides. The experiment aimed to examine a number of human DNA polymorphisms, the PV92 Alu polymorphism and the mitochondrial control region using human DNA. This was achieved by isolating the mitochondrial DNA from the mouth cells ...

Introduction

Most of the world’s chronic diseases are preventable by long term dietary behavior. Out of the ten major causes of deaths for Americans, diet is a contributing factor to at least five of them. These diseases are such as cardiovascular diseases, cancer and diabetes. A report by the Joint World Health Organization on diet made in 2003 revealed that, most of the world’s chronic diseases can be prevented by regular physical exercise and maintenance of a healthy weight. This finding is valid up to date. According to this report, a person’s genome determined their susceptibility to disease while external ...

Lab 1: Ethical Issue in the Movie Gattaca. The epicentre of ethical issues in the movie Gattaca is the manner in which an individual ought to be judged. Should it be by the genetic composition one is born with or by the character traits, personality attributes and ambitions chosen by an individual? Vincent is born with genetic weaknesses that could result in ailments in the future. His genetic composition denies him access to his dream job despite the fact that he achieves the required grades in school and is a strong and athletic young man. As a result, Vincent designs a plan to ...

Introduction

The study explored the homological characteristics of 22 species. The organisms were human, pygmy chimpanzee, common chimpanzee, gorilla, orangutan, white-handed gibbon, macaque (Cercopithecine monkey), horse, bison, cat, dog, bird, bat, frog, rattlesnake, alligator, fish, whale, wolf, pig, bacteria, and cow. The species were categorized as mammals, reptiles, birds, fish, or amphibians. However, humans and apes were further classified as primates. Moreover, the research sought to determine whether the species share characteristics that link them to a common ancestor. The research hypothesis was that if the evolutionary path of the 22 organisms were traced, then a common ancestor would be observed.

Universal Genetic Code

The ...

- Why are mutations at D185, D186 and D110 are so detrimental to RT activity? (15 Points) Mutations at D185, D186 and D110 are very detrimental to RT activity owing to the fact that the three carboxylate residues have been documented to participate in some forms of physiological processes and metal coordination function that is fundamentally required in the binding of deoxyrobonucleotide substrates to the enzyme. And so, when these subunits are interfered as a result of mutation then the fore mentioned biological process are essentially incapacitated. Furthermore, the region was also subcloned due to mutagenesis hence the high ...

#1. Observations Human beings are morphologically diverse. Compared to other primates, human beings show less variation. We differ considerably in our external appearances (eye color, hair color, skin color, hair texture, skin texture, blood group types- A,B, AB and O, height, build, facial features, etc) and our physiology. However despite all these variations, the genome of each and every human being is 99 percent identical (Lai CQ, 2010). It is also interesting to note that humans and chimpanzees share 98 percent of the genome. The study of these genetic variations is very important to geneticists. The allelic ...

Abstract

Human XPA is a protein engaged in DNA damage recognition and repair. A mutation in the gene encoding the protein causes an autosomal recessive disorder, xeroderma pigmentosum (XP), which renders an individual sensitive to sunlight and causes skin cancer. XPA is a 273 amino acid protein, which is predominantly localized in the nucleus. It is expressed at both high and moderate levels in several tissues. The protein serves as a damage sensor in a nucleotide excision repair mechanism, through which the damage to a DNA strand is verified and repaired by formation of a newly synthesized strand. Besides this primary NER mechanism, XPA in conjunction ...

(Author)

Use of Restriction Enzymes in Pharmaceuticals Restriction enzyme, which is also referred to as restriction endonuclease, is a class of enzyme that is helpful in cutting DNA at restriction sites. Restriction sites are particular recognition nucleotide sequences on double stranded DNA having the length of 4-5 base pairs. Restriction enzymes are also known deoxyribonucleases (DNases). Presently, there are four different types of restriction enzymes: - Type I enzymes that are “classical” in nature, e.g. EcoKI, EcoBI, and EcoR124; - Type II enzymes that are “orthodox” in nature, e.g. EcoRI, HindIII, EcoRV; - Type III enzymes ...

Objective 2

Introduction 2 General background 3 Resistance mutants 4 Hemoglobin mutants 4 Sickle Cell Anemia and Genetics: Background Information 5 Connection of Sickle cell with Malaria 6 Method: 7 Introduction of Recombinant DNA technology in diagnosing disease: 8 Discussion (implementation of recombinant DNA technology to solve the issue) 9 Conclusions 10

References 11

Objective This report targets to study a group of people that has developed resistance to malaria. It will focus on the use of recombinant DNA technology to study this cohort and gain insights into the nature of this observation. This ...

Mental disorders presently affect a large proportion of world population. The World Health Organization (2012)conducted a status survey, which found that a significant chunk of the world population suffers from various degrees of a wide assemblage of mental disorders. According to the World Health Organization Report (2012), over 350 million people suffer from depression all over the world. In a conflict between, Nature vs. Nurture, scientific community stands divided on the contribution of genetics on Psychiatric disorders and illness. However through this essay, I will put forward arguments based on scientific findings that showcase the contribution of genetics on mental disorders. Unlike ...

Introduction: Genetic mutations are associated with changes in the genomic sequence that are the main cause of diversity among organisms. The changes in genetic sequence can occur at any instance and are known to have different consequences. Mutations are often associated with a change that affects the nucleic acids in most organisms. Nucleic acids are considered as the building blocks of DNA. On the other hand, DNA are associated with the storage of information that is transferred to the offspring during reproduction. Mutations are associated with diversity and considered as an essential part in evolution. Genetic mutation is considered to ...

Abstract

This research paper will discuss the significant contribution of DNA fingerprinting in the criminal justice system for being able to provide a reliable basis for conviction. DNA fingerprint analysis is referred as an accurate indicator to determine the guilt and innocence of an accused. The history, importance and various DNA fingerprinting techniques shall be discussed. In addition, DNA fingerprinting is considered as more superior than other forensic science methods since the technology has improved over time. DNA fingerprinting has allowed the test of smaller amounts of material, faster testing procedures and more conclusive results. Finger ridge analysis is science and ...

Abstract

Obesity is a heritable and genetically influenced disorder. Recent studies have proved that obesity is a biochemically driven neurological disorder. Obesity has a complex and heterogeneous genetic and phenotypic manifestation. Familial obesity is monogenic and common obesity is polygenic. Leptin and its receptor (LEPR), the α-melanocortin-stimulating hormone receptor (MC4R), pro-opiomelanocortin (POMC) and prohormone subtilisin/kexin type 1 (PCSK1) are some of the single genes involved in monogenic obesity. Glutamate Decarboxylase 2 gene (GAD2) gene is thought to be part of a polygene system resulting in common obesity. GAD2 coding sequence is present on chromosome 10p locus and encodes for a protein GAD65. This protein catalyzes ...

1.0 Introduction Hemophilia A (HA) is an inherited X-linked bleeding disorder that results from a wide range of mutations in the factor VIII (FVIII) gene which leads to the absence or quantitative and/or qualitative deficiency of the said blood clotting factor ( (Jacquemin, et al., 2000; Guillet, et al., 2006; Venceslá, et al., 2008). FVIII gene is one of the most extensively investigated of all human genes and these extensive studies have led to the identification of a wide range of different types of mutations responsible for HA. Some of the mutation types identified include point mutations (resulting in missense, nonsense ...

The advancement of technology has precipitated a revolution in scientific understanding over the last several decades. Advancements in research tools has led to new insights into every facet of scientific discovery within the realms of chemistry, physics, and biology and all the sub-disciplines forming their constitution. Increased discovery has brought with it opportunities for cross discipline cooperation which has resulted in the formation of new disciplines and has galvanized scientists to press the boundaries of research and application as has never been seen in human history. One such field testing those limits is genetics. Today’s geneticists have expanded beyond ...

- Abstract Cardiovascular diseases such as heart failure are some of the leading causes of mortality and morbidity with Australia being one of the country’s most greatly affected (McLean, Esclick & Coats, 2006). An area of interest in the pathology of heart failure is the significance that polymorphisms of adrenergic receptor genes have on the outcomes of patients with heart failure. Single-nucleotide polymorphisms or SNPs that occur in the genes that encode for adrenergic receptors are known to have various physiological and functional consequences. Specifically, the β1-adrenergic receptors have a crucial role in the regulation of heart rate and ...

Molecular Diagnostics

Genomic Evaluation of Risk of Atrial Fibrillation and Alzheimer’s Disease

Session You Attended

Abstract The aim to of this series of experiments is to examine a patient’s DNA for association to atrial fibrillation (AF) and Alzheimer’s disease. The single nucleotide polymorphism (SNP) rs2200733, associated with an increased chance of development of atrial fibrillation. Two SNPs: rs439358 and the rs7412, located within exon4 of the human apolipoprotein E (APOE) gene are associated with increased risk of developing Alzheimer’s disease. Isolation of the DNA from patient white blood cells, followed by PCR-RFLP and cycle sequencing are among the techniques ...

Introduction

The emergence of real-time polymerase chain reaction (PCR) assays was much of a relief in the medical field especially given the fact that this model offers the most accurate way in the process of determining false-negative outcomes (Burggraf & Olgemoller, 2004).This ideology also finds support in the works of Nolte (2004) who asserts that, following the discovery of PCR and its application in a clinical setting, it has become possible to use nucleic acid amplification methods as transitions from research to clinical laboratories. This makes it possible to have a wide array of application in addressing issues relating to genetics, pharmacology, oncology ...

Chloroplast and mitochondria are membrane bound organelles found in eukaryotic cells’ cytoplasm. Chloroplasts are present in all multicellular plants and some algae species while mitochondria are found in most eukaryotic cells. Their main similarity is that both of these organelles generate the universal cell energy carrier known as ATP (Adenosine Triphosphate). - Chloroplast DNA Geneticists have long established that many chloroplast associated traits display cytoplasmic inheritance. This suggests that nuclear genes are not responsible for encoding of these traits. In 1963, it was discovered that chloroplasts have their own deoxyribonucleic acid (DNA) known as chloroplast DNA (cpDNA). ...

39 A. When a female tortoise shell cat mates with a black male cat, the resulting offspring that could be produced have the following genotypic frequency: XBXb: XBY:XbXb:XbY with a 1:1:1:1 ratio. 39 B. This is the result of mosaic expression. Female tortoise shell cats have a mixed coat color in heterozygous individuals. This due to the expression of both the dominant allele (XB) resulting in patches of their coat being orange and the recessive allele (Xb) which results in patches of their coat being black. This is a result of one of the X chromosomes being inactivated during development. So some epithelial ...

What are the causes of muscular dystrophy in the elderly?

1.0 Introduction Muscular dystrophy refers to a group of degenerative disorders that are hereditary and progressive which result in the weakening of the skeletal muscles. There is a variety of muscular dystrophy which differ in terms of onset, degree of severity, penetrance and pattern of muscles that are affected. The main types of muscular dystrophy include: facioscapulohumeral, congenital, myotonic, Duchenne, limb girdle and oculopharnygeal and distal. Some of these disorders manifest during the formative years of life but some only manifest later on. This therefore implies that muscular dystrophy is a group of degenerative disorders that affects people of all ages; ...

According to Shortliffe and Cimino (2006), Bioinformatics enables students understand the processes involved in biotic systems. Biotic systems support in the study of how complex social systems interact by accumulating information, forming restrained behavior and maintaining prebiotic evolution models. Shortliffe and Cimino assert that developing this database we need to develop and design complex interfaces that researchers use to access the available data, revise it and then submit the data (2006). Bioinformatics helps to study, analyze and interpret data that involve amino acid and nucleotide sequence. Using of the computer has also improved the study of bioinformatics hence enabling ...

There are four bases forming part of the DNA: cytosine, adenine, thymine, and guanine. The bases link to form a nucleotide. This comprises of a pentose sugar, an organic base and a phosphate group. Each combination of these nucleotides is a codon. The sequencing of nucleotides affects the amino sequence since each codon is translated into a different amino acid. This means that each combination represents a different amino acid (McConkey 24).

Addition or deletion of a single nucleotide somewhere in the sequence results in formation of different amino acids from the point of addition. This is because the addition or deletion affects the polypeptide-coding segments. ...

Introduction

Genetic evolution refers to change(s) in inheritable traits occurring across succeeding generation of organisms. The change (evolution) is responsible for the diversity at all levels including the molecular level e.g. genetic variations, individual organisms in the same species (intra-species variations) and among species (interspecies variations). It is believed that all organisms emanated from common ancestry about 3.7 billion years ago and then evolved over time to the present biodiversity. Therefore all organisms have some shared traits at the morphological and molecular level with the species sharing a more recent ancestry having more similar traits. The process of evolution involves ...

When people become sick with dermatomyositis (DM) they have an inflammatory skin rash and inflammation of the voluntary muscles. The patients experience muscle weakness which gets worse.

The DM most often starts with a speedy, severe onset of symptoms. Patients eventually become so weak they have trouble with daily routine activities such as standing up after sitting down, climbing stairs or lifting objects. Other organs such as the lungs may also become diseased.

The symptoms are often treated with prednisone which is a corticosteroid drug used to decrease the inflammation. Other corticosteroid drugs can be used but that family of drugs ...

Pharmacogenomics alternatively referred to as pharmacogenetics in medical literature is a component of personalized medicine. It refers to the study of genetic polymorphisms that in one way or another influence individual response to drugs. Clinical observations on the effects of genetic polymorphism on the pharmacokinetics and pharmacodynamics of drugs were first documented back in the 1950s. Scientific knowledge on genetic variations is therefore the critical foundation on which pharamacogenomics is based. In specific, the knowledge of how variations in the genes responsible for encoding enzymes and proteins are the main pillars of pharamacogenetics (Belle, 2008). Enzymes play a vital role in ...

Telomerase also known as telomere terminal transferase is a type enzyme in all vertebrates that extends Die ribonucleic acid sequence by repeating TTAGGG to the Die ribonucleic acid terminal strands in the region known as the telomere. It is found in adult germ cells, fetal tissues, and tumor cells. Telomere is the region of repeated nucleotide, which is made up of non-coding Die ribonucleic acid material. The region prevents loss of vital Die ribonucleic acid from chromosome terminals. This results to only a hundred to two hundred nucleotides being lost each interval the chromosome is duplicates, thus no damage is caused ...

Part A.

There has been at least a dozen various human like species since the earliest homid species split from the ancestral line the humans shared with the apes. This was approximately 6 to 8 million years ago. Most of these homid human like creatures are just relatives to the human beings and are not their ancestors. Humans are more closely related to modern apes like gorillas and chimps more they are to the monkeys. It is believed that humankind shares a common ancestry line with the African apes, like chimps and gorillas. And it is believed that this common line of ancestry ...