Gel College Essays Samples For Students

Principle Gel Electrophoresis is a method used in the laboratory for the separation of charged molecules like nucleic acids (DNA and RNA) and proteins that differ in size or charge (Agarose gel electrophoresis (basic method)). LB (Lithium Borate) buffer is the widely used buffer in agarose gel electrophoresis. The buffer solution comprises of Lithium hydroxide monohydrate and boric acid. LB buffer has low conductivity which can run at a high speed (5-50V/cm) than the gels which are made of TBE or TAE buffers (5-10 V/cm) which in turn reduces the run time drastically (Brody 6).

Experimental Required

Materials: Gel Electrophoresis Unit (Gel casting ...

I confirm that this work is my own work. Where I have rewritten someone else's ideas in my own words, I have identified this with a reference.Where I have used words directly from a book or any other source, I have enclosed these in quotation marks and have identified the source in the reference section.

Question 1

Below is a photograph of gel electrophoresis result obtained from the experiment.
Figure 1
L G-NTC G-DNA G-P H-NTC H-DNA H-P
The gel electrophoresis result showed 14 visible bands from the lane loaded with the ladder. No visible band was observed on the lane ...

Introduction

Tooth development involves a complex process whereby teeth are formed from embryonic cells, and then eventually mature after erupting as evident in the mouth (Eckhardt, Jágr, Pataridis, & Mikšík, 2014). The development usually originates from mesoderm and ectoderm. At the ectoderm found in the oral cavity, there is the development of enamel and other tissues also come from associated mesenchyme. All teeth don’t develop at the same time, but rather in stages (Eckhardt, Jágr, Pataridis, & Mikšík, 2014). First buds are always observed in the region of anterior mandible, followed by anterior maxillary area then finally ...

The bio freeze pain relief gel is a product that has been identified as a famous cold therapy pain relieving product. According to Biofreeze (2016), the bio freeze pain gel relief is considered as a product that reduces and breaks the cycle of pain. This essay brings to light the SWOT analysis for the product; therefore, analyzing its strengths, weakness, opportunities, and threats involved with using the product.

Strengths

The strengths of this product revolve on the ability that is has in providing relief from minor aches. These pains are common among athletes who have muscle injuries most often. The ...

Introduction

Medicinal benefits of Aloe Vera Juice
The extent this information available to the public
Scientific proof of the medicinal qualities of the herb/ aloe vera juice
Conclusion
Abstract
This essay seeks to establish the premise that aloe vera juice contains a wide range of medicinal properties, which scientist up to this time ignore. Presently the juice is being used as a purgative. However, it is this author desire to explore its true identity in providing data supportive of the medicinal benefits contained in Aloe Vera Juice. Also an examination into the information related to aloe vera ...

The current paper is devoted to the analysis of the discovery or the invention of Blue-Native Polyacrylamide Gel Electrophoresis (Blue Native PAGE). As well, the paper undertakes to find out the people who were behind the discovery. The paper is besides devoted to highlighting the functions or rather the reasons that prompted the inventors to come up with Blue Native PAGE.
Blue-Native Polyacrylamide Gel Electrophoresis (Blue Native PAGE) was at first described by von Jagow and Schägger. They initially discovered Blue Native PAGE in 1989 as a simple technique to isolate membrane protein complexes immediately from purified mitochondria. ...

Introduction

The following is a critique of both qualitative and quantitative research studies. The qualitative critique covers an article titled “Vulnerability in perioperative patterns: A qualitative study” by Cousley. The article properly presents the listing of factors that cause vulnerability in perioperative patients as well as the use of alternative techniques to ensure qualitative vigor is achieved. The weakness of the article is that it used only ten participants and it relied on a single interviewer.The quantitative critique covers the article “Sore throat in women after intubation with 6.5 or 7.0 mm endotracheal tube: a quantitative study” by Gustavson et ...

Introduction

Decubitus ulcer also referred to as bedsore or pressure sore is an open wound on the skin (Thomas, 2006). Thomas (2006) indicates that the decubitus ulcer mostly occurs on the skin that covers the born areas. Notably, the most common areas for bedsore to appear are; hips, ankles, back and buttocks. Decubitus ulcer are common among the disabled, elderly and those people who spend long periods of time on wheelchairs or bed, and those who are not able to move certain parts of their body without assistance (Thomas, 2006). In keeping with Albert and Spencer (2007), decubitus ulcers/ bedsores ...

Affiliate institution

First article
According to the article, treatment of poisoned patients in a poison center is much cheaper as compared to poisoned patient treated in the hospital. Furthermore the patients treated in the poison centers takes shorter time to recover. The study compared the data obtained from Illinois Poison Center and data from the Illinois Hospital Association, and the results show that for every 10 patients treated in Poison centers there is a decrease in hospital charges by $2078 and the length of stay for poisoned patients was .058 days shorter. Notwithstanding the study finding that poison centers have shown to ...

Question 1

a)
i) If genomic DNA from the wild type bacterium were digested with EcoRI, three different fragments would be detected. The approximate sizes of the fragments would be 1.625 Kb, 1.375 Kb and 0.25 Kb. This is because there are two different digestion sites for EcoRI within the region complementary to the probe, and therefore three different fragments would partially hybridize with it after the digestion.
ii) There are only two restriction sites for BamHI shown in the diagram, but neither of them is within the region complementary to the probe, and both of them are upstream of this ...

Protein degradation is a regulatory process of all living beings that helps to weed out proteins that are faulty in nature. The fault maybe in their sequence or folding. The proteins are degraded via ubiquitin-proteasome system (UPS). The proteasome is made up of an outer activating module and an inner core particle (CP), which in turn is made up of outer α subunits and inner β subunits. The α subunits guard a N-terminal gates pore, which open up when proteasome activators (PA) binds to it. The β subunits have the active sites that can broadly accommodate different faulty proteins for degradation. ...

The experiment aimed to show that Blm10 protein elevates the turnover of proteasomal substrate through an active gating mechanism. This was to be done by showing that Blm10 enhances degradation that is proteasome-mediated in vitro using hypophosphorylated unstructured tau-441 as the substrate.

The objective was achieved using various stains such as BY4741, yMS63, yMS94, yMS122b, yMS131, yMS152, yMS159, and yMS476 among others. To start with, the proteasomal activity was assayed by determining gate opening in yeast CP/20S proteasome by the C terminal peptides. Phenotypic analysis was also done by growing the stains overnight in YPD and diluted to a density ...

The Turkish independence war led to the birth of the Republic of Turkey. The post-colonial government was expected to be a bastion of freedom and civil liberties. The Ottoman Empire had been defeated in the First World War, and the Sultanate abolished. The Turkish people formed the Turkish National Movement to wage war against the allied nations to grant independence to Turkey. A nationalistic government was formed in 1923 after the Treaty of Lausanne, which led to the establishment of the Turkish Republic. After independence, the government was considered to be despotic in relation to the human rights. The Kurdish ...

Virtual Lab Report

Virtual Lab 1: Virtual Microscopy
A. Gauge the size of various biological components and organisms. The Virtual Microscope (copy and paste this address into a new browser window: http://learn.genetics.utah.edu/content/cells/scale/ ) can be used to make these observations. Estimate the size (length and width in microns) of
An E.Coli cell is around 0.5µm in width and around 2µm in length. A mitochondrion is around 0.5µm in width and approximately 10µm in length. A Red blood cell is around 5.5µm in width and 7.5µm in length. A virus is approximately 0.25µm in width and ...

Medical technology is a term that talks about the usage of a medical device to prolong life, ease pain and or decrease the hazards of a disease (Fullick, 2012). There are a number of technical discoveries that are used globally in medical facilities to enhance health care. Technology has positively impacted the lives of many individuals worldwide. Medical procedures such as surgeries and medications are all included under the technology advancement category. Owing to the vast technological development, doctors are able to perform treatments that could not have been possible earlier (Haidekker, 2013).
One of the impressive technologies used in the medical ...

Donut hair band is one of the most popular accessories for creating modern hairstyles. Donut hair band resembles a well-known baked food – a donut, which is why it is called this way. Stylish and practical donut bun is a universal solution for many occasions.
The bagel or donut bun is a versatile option hairstyle for medium and long hair. It is convenient to wear to work, school, celebration, for a walk with your friends - a variety of embodiments of this hairstyle is enough today. You can create a perfectly smooth donut or an easy casual, with protruding strands, ...

The media categorizes everyone into certain cultural groups based upon what they consume. Consumer behavior, whether it be in the form of products and/or media content, determine how the media views our consumption patterns, and determines which cultural group we belong to. However, our self-perception may not always match the profile of our cultural-consumption. In my case, I think that my self-perception, with a few notable exceptions, was gauged poorly by my cultural-consumption profile.
First, my gender is male, and my consumption behavior is vague with respect to gender preferences for certain products. For example, my list shows that ...

(Name of the class)
(City, State)

Introduction

TLC or Thin-layer chromatography refers to a technique that is applied in the separation of mixtures that are not volatile (Reich & Schibli, 2007). The process is done on a sheet of plastic, glass, or aluminum foil that has been coated using a thin layer of material that is adsorbent such as aluminum oxide, silica gel and cellulose (Sherma & Fried, 2003; University of Colorado, 2014). This adsorbent layer is referred to as the stationary phase. The sample is usually applied using a capillary tube on the plate, and the sample is taken up the plate through capillary ...

For generations, women have been looking for the secret to younger and beautiful skin. Different beauty products are available to make women feel better and more beautiful. However, women may not know that these products may have harmful ingredients that may cause skin irritation. There are also some chemical-based products that harm the skin with continuous use. Chemical-based beauty products may no longer be needed because Aloe Vera is here to help women in taking care of their skin. Proven safe and effective, Aloe Vera will keep women’s skin younger and fresher. The Aloe Vera, aside from its ...

On Whey Protein Isolates

The hardness and brittleness are key characteristics of protein molecules that can be used in determining the nature of the proteins. The hardness of the protein molecules refers to how well the molecules are bonded together making it more difficult for the denaturation of the protein molecule (Vardhanabhuti, & Foegeding, 1999).On the other hand, the brittleness of the protein molecule refers to the character of the protein molecule that prevents breakage in the bond linkage when subjected to different conditions.
A major function of proteins among the food systems is the process of gelation. The process constitutes a ...

Product description

The product to be designed is called Dry Space. This product is a dedicated type of gel pad which can be used on the restroom floors. This item is used to help the users dry faster once they leave the bathrooms. A user will step onto the device and excess water will then drain into a receptacle placed at the base of the device. This device has the advantage of helping the user remove the frequent unhygienic conditions that usually form on the floors of the bathrooms when a person has showered. This device was built to solve the problem of removing ...

INTRODUCTION

General overview

Preparation of yoghurt takes place through milk fermentation process with various cultures of bacteria comprising of streptococcus subspecies mixed with lactobacillus delbrueckii subspecies and thermophiles (Barbano, 2009). Basically, yoghurt comes in two types: distilled and set (Augustin et al., 2003). The author further explains there is a difference in the main procedure through which these yogurts are manufactured. In the set yoghurt (inclusive of fruit on the bottom), the formation takes place in retail pots as the fermentation of the lactic acid bacteria proceeds into lactic acid generating a structure of continuous gel in the container of the consumer. ...

Abstract

The field of Nanoscience has evolved due to the other advancements in the other fields of study such as the Nanotechnology. While the latter is a subset of the former, there is credible evidence that points to the fact that Nanotechnology has been developed thanks to various areas such as biology, material science, engineering physics, chemistry and much more. It is the foundation on which the Magnetic Nanoparticles have been embedded. MNP is nanoparticle that has the magnetic manipulation ability.
Thus, this paper focuses on the study of the Magnetic Nanoparticles especially how they are formed or synthesized and ...

Introduction

Atherosclerotic cardiovascular disease (CVD) involves peripheral circulation, the brain, and the heart. The disease is a primary health issue in many countries around the world (Sawhney, et al., 2012). CVD develops through complex processes involving novel risk markers and common risk factors. Decades of research have demonstrated that a sibling’s or parent’s family history is linked to the development of atherosclerotic CVD (Gupta et al., 2013; Nelson, 2013; Imes and Lewis, 2014), which manifests as peripheral arterial disorder, stroke, and coronary heart disorder (CHD). Various Mendelian disorders have also been shown to contribute to atherosclerotic CVD. Although ...

Abstract

In the last century, the knowledge of molecular biology has increased, opening new opportunities for addressing different diseases that have been affecting all populations. Before, genome and proteomic studies were seen as complicated field due to inability to get significance understanding of disease components at the molecular level. However, with the expansion of molecular biology, different techniques and tools have been developed to aid diagnosis and treatment of diseases. In this study, these tools have been investigated to understand how the knowledge on molecular diagnosis helps in detection and treatment of diseases. This study focused on the application of ...

Human Physiology ECG Laboratory Report

Abstract
This is a practical experiment on ECG concerning human physiology. The experiment intends to achieve one primary goal: equip the learner with vital skills pertinent in making rational decisions anchored on scientifically proven facts. I chose to study participants from both sexes with varying levels of nutritional statuses and physical activities. We recorded the foods consumed by each participant. The results indicated that those who took caffeinated drinks positively correlated to emission of rapid ECG signals demonistrated by the ECG readings.

Introduction

The heart is a fundamental organ in sustaining life. It contracts at regular and perpetual intervals throughout ...

Introduction

Resource curse has been one of the most debated economic issues for the past four to five decades. Indeed, it refers to a theoretical approach that connects certain indicators of a country’s economic growth to its reliance on natural resources such as oil, gas, and minerals. According to a general belief (termed as ‘resource curse’), the countries that are rich in natural and non-renewable resource are characterized by lower levels of democracy, economic growth, and development as compared to the countries with less or no reliance on these resources (Sachs, and Wartner, 2001).
On the basis of the ...

LIBs Summary and Prospects

Research in lithium ion batteries (LIBs) has focused on both active and non-active components. Flexible packaging of LIBs has been achieved by using aluminum pouch cells, polymer-coated aluminum pouch, and polymeric pouch cells. Polydimethylsiloxane material has also proved to be an excellent material suitable for packaging. Research in flexible materials has incorporated the element of cost. Laminate structures have been found to be less costly than the current technology in use. Separators for LIBs need to be not only thin, but also highly porous Cellulose nano-fiber paper-derived (CNP) and ceramic-based separators have shown impressive performance. Manufacturing of LIBs requires ...

{Author Name [first-name middle-name-initials last-name]}
{Institution Affiliation [name of Author’s institute]}
A cell is a form of the miniature factory that encloses a distinct chemical system that is involved in structural maintenance, homeostasis, energy generation and reproduction. Every organism is made of these structural living units. The cell divides and produces more cells and assists in the growth of the organism. There are two types of cells, eukaryotic and prokaryotic. Eukaryotic cells are characterised by a membrane-bound nucleus enclosing the genetic material. The Eukaryotic cells can be divided into three main parts, plasma membrane, cytoplasm, and nucleus (Cooper & ...

The ultimate goal of every medical practitioner is to provide the most efficient services to the patients. It happens to dentists who look forward to giving satisfactory and durable restorations. For a fruitful repair, Monolithic Zirconia crowns are used. A Zirconia crown is a type of ceramic crown that is worn to give a better appearance of the tooth that has been disfigured or stained for quite some time. The crown is made from a durable material that cannot be rejected by the human body (Stober et al., 2014). Zirconia crown is also used for other medical purposes such ...

Abstract

This is a practical experiment on ECG with regards to human physiology. The experiment was intended to achieve one primary goal: equip the learner with vital skills pertinent in making rational decisions anchored on scientifically proven facts. The alchemist recruited study participant from both sexes with varying levels of nutritional statuses and physical activities. The foods consumed by each participant were recorded. The results indicated that those who took caffeinated drinks positively correlated to emission of rapid ECG signals demonistrated by the ECG readings.

Introduction

The heart is a fundamental organ in sustaining life. It contracts at regular and perpetual ...

Biology

Abstract
This is a practical report on human physiology EMG. The experiment recruited 85 subjects to examine the level of the signal emanating from muscle contraction in the lab. Some of the participants were left-handed while some were right handed. Males and females with varying levels of activity were recruited. The results indicated that more males exhibited maximal compression force than females as attested by the higher numbers that returned quickly to half the clench force. The level of activity of the participants positively correlated to slow return to half of the maximal compression force and reduced signs of ...

Objective

Fibre reinforced plastics (FRP) have demonstrated suitability for construction projects due to their strength and durability, while weighing very little. The attributes of FRPs were examined as well as, their applicability in hot, desert temperatures for road barriers and other road safety units.

Background – Composite Materials

Strength and stiffness are the main mechanical properties fibres add to composite materials. The fibres must have uniform diameter, similar strength between each fibre, and very large length-to-diameter ratio. Crystals within the fibre that are aligned with fibre length create high tensile strength. Fibres need to have air space and fibre packing needs to be random ...

Question 1

Stress and blood sugar levels are closely linked. Stress may be caused by various environmental factors. This is especially common in today’s society that comprises of things occurring at high speeds. When the body is under severe stress, the body releases stress hormones, such as cortisol. Stress hormones cause an automatic increase in the level of blood sugar. Adrenalin, which is released during instances of flight and flight, has a similar effect. The main reason for this kind of functionality is to enable the body to have sufficient energy to react to the cause of the stress.
Under ...

Introduction: Genetic mutations are associated with changes in the genomic sequence that are the main cause of diversity among organisms. The changes in genetic sequence can occur at any instance and are known to have different consequences. Mutations are often associated with a change that affects the nucleic acids in most organisms. Nucleic acids are considered as the building blocks of DNA. On the other hand, DNA are associated with the storage of information that is transferred to the offspring during reproduction. Mutations are associated with diversity and considered as an essential part in evolution. Genetic mutation is considered to ...

Deploying new technologies will be affected by political and regulatory dimensions. Politics will affect the deployment of new technology. This is evident with the various policies that have been put by governments concerning the various dimensions of technology (Cheng, 2013). Both local and international perspectives of technology deployment will have to be considered in the new technologies (Coşgel, Miceli, & Rubin, 2012). This is an vital process that needs to be considered in the deployment process. There are processes that have to be followed before new technological applications have been approved by the government. It is because of these ...

SITUATIONAL ANALYSIS

Gillette India Limited
Personal Care Products Market in India
Targeting a wider Consumer Base with Personal Care Products

- General Environment- Gillette India Limited and the Indian Personal care Products Industry
- Political and Legal Environment
- Economic Factors
- Sociocultural Factors
- Technological Environment
- Porter’s 5 Forces Analysis
- Suppliers
- Buyers
- Substitutes
- New Entrants
- Rivalry
- Personal Care Products Industry Trends in India
- SWOT Analysis
- Key Success Factors
- Decision Criteria/Recommendations
- Looking Forward- ...

A Brief Review of Epogen

Epogen is a glycoprotein made up of 165 amino acids that is used to stimulate the production of red blood cells. The compound is manufactured through recombinant DNA technology. The compound has a molecular weight equal to 30,400 Daltons. When consumed, its biological effects are similar to those of endogenous erythropoietin. The dugs formulation is a sterile colorless liquid in a buffered solution. It is administered intravenously or subcutaneously. This drug is used by athlete to enhance their performance. This is by producing more red blood cells and opening up lung passages so that more oxygen is delivered to the muscles. Endurance ...

(DNA Quantification)Mitosis and Meiosis) (Genetics)
1. At 360nm, the DNA strongly absorbs light and the value generated allows easier estimation of solution concentration.
2. Contamination of RNA can easily result in an overestimation of DNA concentration due to high occurrence of RNA secondary structures.
3. The cell wall of plant cells are surrounded by a cell membrane. In this cell the DNA is located in the nucleolus and enclosed by a phospholipids membrane. In plants, the phospholipids tend to exist in the diacyl form.
4. The variations in DNA are affected by the number chromosomes in ...

Methodology

The first step involved ligation of digested polymerase chain reaction (PCR) products into pGLUX1 proteins. Thereafter, the resultant proteins were transferred onto nitrocellulose filter membrane (NCF). This is where the ligation reaction was used to transform E. coli XL10-gold cells. Thereafter, specific cells were selected to undertake DNA plasmid isolation through being subjected to 200 volts for 50 minutes. In confirming complete ligation of the cells, restriction digest using XhoI and KpnI enzymes were used. The digested products were then subjected to agarose gel electrophoresis. In this case, the transfected culture was grown at 37°C under drug selection ...

The following paper describes the current developments and advances in the use and application of smart packaging technology in food packaging processes. The paper focuses on the application of the technology through various methods of smart food packaging, process designs, equipment used and the current applications. The paper also gives the various applications for the smart food packaging technologies.

Oxygen Absorbers

Oxygen absorbers/scavengers refer to smart packaging technologies that work by absorbing oxygen from foods while reducing oxygen metabolism in the packaged food (Latou et al. 2010). The process is designed to decrease oxidative rancidity and stop any unintended oxidation of ...

Pharmacogenomics alternatively referred to as pharmacogenetics in medical literature is a component of personalized medicine. It refers to the study of genetic polymorphisms that in one way or another influence individual response to drugs. Clinical observations on the effects of genetic polymorphism on the pharmacokinetics and pharmacodynamics of drugs were first documented back in the 1950s. Scientific knowledge on genetic variations is therefore the critical foundation on which pharamacogenomics is based. In specific, the knowledge of how variations in the genes responsible for encoding enzymes and proteins are the main pillars of pharamacogenetics (Belle, 2008). Enzymes play a vital role in ...

Introduction

The collection of bacterial, archaeal, eukaryal and viral microbes in surface waters, oceans soils and wastes together constitute the environmental microbiome. This microbiome is vital to humans and the environment in several ways including but not restricted to nutrient recycling, biotechnological application in the food and beverage industry and genetic engineering technologies. Nonetheless, pathogenic microbes can invade and grow within other organisms causing disease (Egli and Koster).
Different strategies have been used to define microbial community structure across microbial ecosystems. Taxonomic classification based on morphologic and physiological traits of cultured microbial communities is no longer attractive since the ...

Introduction

The collection of bacterial, archaeal, eukaryal and viral microbes in surface waters, oceans soils and wastes together constitute the environmental microbiome. This microbiome is vital to humans and the environment in several ways including but not restricted to nutrient recycling, biotechnological application in the food and beverage industry and genetic engineering technologies. Nonetheless, pathogenic microbes can invade and grow within other organisms causing disease (Egli and Koster).
Different strategies have been used to define microbial community structure across microbial ecosystems. Taxonomic classification based on morphologic and physiological traits of cultured microbial communities is no longer attractive since the ...