Essays on Stain

Abstract

In the field of medicine, histopathology process is undertaken by pathologist.The aim of undertaking histopathology is to examine the existence or the possibility of a disease in the body tissue of a living thing. In addition, the process of histopathology is best practiced on the specimen of body tissue taken during the surgery of a patient. Here, the process involves a series of activities where surgery helps to remove body tissues for examination. After the surgery, the body tissue removed is placed in a fixative place where no external toxicities can contaminate the specimen. This forms the basis for ...

Introduction

Blood behavior conforms to specific scientific principles and therefore, professionals trained to perform bloodstain pattern analysis can analyze the blood evidence in a crime scene and come up with conclusions as to how the blood shedding happened. The stains may appear to have a random distribution in a crime scene, but the analysts can study the spatter patterns, transfers, and other marks. From the physical evidence, the investigators recognize and interpret the patterns to determine how they were created. These marks are then categorized to recreate the sequence of events that occurred following the bloodshed. Blood stain pattern analysis, ...

Chartres, France

The monuments and architecture in ancient times allow talking and narrating about the historical background from the angle of time in which they were built. Through various signs, they inform us about the prosperity, decline of the reigns and empires as well. Thus, they are the major means of communication. They can tell us a lot about the economic, political and religious circumstances of the specific region. They also narrate the contemporary architectural trend. In medieval Europe, especially in France, the Gothic architecture was at its pinnacle. Cathedral Notre-Dame de Chartres is one of the Gothic architecture examples in ...

INTRODUCTION

Klebsiella pneumoniae belongs to Klebsiella species of Enterobacteriaceae and constitutes the normal flora of the human mouth and intestine. K. pneumonia is the most predominant and clinically critical organism. Infections with K. pneumoniae are mostly contracted from the hospitals and principally occur in patients with damaged host defenses. The objective of this study is to perform techniques to identify the bacterium in the given sample and confirm its presence by staining and microscopic analysis.

Streaking may be performed on the clinical sample using Mac Conkey agar or blood agar with the goal of isolating colonies of the organism from ...

What are three major causes of chronic bronchitis?

The three major causes of chronic bronchitis are cigarette smoking, long time exposure to air pollutants (such as industrial or chemical fumes), and inhalation of toxic gasses (Madell, 2016).

What are the major pathologic airway changes are associated with chronic bronchitis?

Chronic bronchitis is the condition affecting human beings that cause a continuous irritation and inflammation of the lining of the bronchial tube. Bronchial tubes are airways that carry air to and from the lungs. Exposure to cigarette smoke, air pollutants, or toxic chemicals causes a persistent cough that leads to the formation of excessive amounts of sticky mucus in the airways that result in inflammation and fibrosis. The mucus blocks ...

The field of microbiology was very limited until the discovery of the microscope, though it did exist in a very practical sense. Though scientists were ultimately wrong about what actually carried and transmitted the plague, for example, they were correct in hypothesizing that it came from an outside source. Between then and now, the microscope was developed, and has been improved upon more and more. Now we have a great classification scheme for prokaryotes (assuming bacteria). It follows the standard domain, kingdom, phylum, class, order, family, genus, and species. The standard for most eukaryotes is also the same for ...

[Your section appears here] [Professor’s name appears here] [Date Appears here]

INTRODUCTION:

Pathologists or microbiologists play a very vital role in the management and diagnosis of any disease. They get immense help by performing certain series of tests which aids them reaching to a conclusion. One such test is called as gram staining which is used widely as the most primitive test for the identification of various bacteria According to Beveridge, the gram stain essentially delineates bacteria into two broad categories. It does that by giving color to these bacteria and bacteria that attains purple or violet color are ...

Introduction:

Transmission electron microscope (TEM) is the type of a microscope that uses a beam of electron rather than visible light to illuminate the specimen. Besides, while light microscope uses glass lens to focus light to the specimen, TEM uses electromagnetic lenses for focusing of the electron beam. TEM has a higher resolving power than the light microscope because the electron beam it uses for illumination has a much lower wavelength than visible light. During the operation of TEM, some electrons passing through the specimen get scattered and disappear from the beam. However, the unscattered electrons reach a fluorescence screen ...

The monuments and architecture in ancient times are capable of talking and narrating the historical background of the respective time in which they were built. Through various signs, they inform us about the prosperity, decline of the reigns and empires as well. Thus they are the major means of communication. They can tell us the economic, political and religious circumstances of the respective region. They also narrate the contemporary architectural trend. In medieval Europe, especially in France, the Gothic architecture was at its pinnacle. Cathedrale Notre-Dame de Chartres is one of the Gothic architectures in the middle age. It ...

Organism # 6

Molecular Biosciences 101 Section # Purpose Materials and Methods Gram Staining: Three circles were marked at the bottom of a clean glass slide and they were labeled as gram(+), gram(-) and unknown respectively. The three labeled circles were then inoculated with microorganism 6 using an inoculating loop. The bacterial smears were then air dried and heat fixed over a gentle flame. The slide was then flooded with primary stain, crystal violet for a minute by placing it on a staining rack. Excess crystal violet was washed off with a gentle stream of water. Thereafter, gram iodine was added and ...

The mycorrhizal symbiosis between fungi and plant is among the prevalent relations in the world ecosystem. Amongst the various categories of mycorrhizal, arbuscularmycorrhizaare for the most part is common within nature because it is predicted that almost 80 percent of all plants interrelate through arbuscularmycorrhizal fungi (Riemann 101). Therefore, it is obvious that arbuscularmycorrhizas study forms a very important concept to grasp the function ability of other global ecological unit. In arbuscularmycorrhizal symbiosis the fungi that penetrates in it belongs to the phylum Glomeromycota. Such fungus are not easy to nurture invirteo, and their arrangement is been positioned on ...

The following analysis represents the sample data of the judges of Hamilton County and the probability of the cases at three different court levels regarding appeals and reversals.

Introduction:

There are over thousand cases that the judges of Hamilton Country need to deal with. The study for analysis of performance has been undertaken to provide evidence to the media whether the judges are performing fairly well or not. The results of the analysis have been obtained from Kristen DelGuzzi, who belonged to Cinncinati Enquirer; the organization responsible for the conduction of the case study taking three years as a time ...

Bloodstain analysis has been studied since the late 1800’s. The study of bloodstain can help in identifying if a crime was committed or if it was an accidental misfortune. A person who analyzes blood splatter patterns should be trained and educated in how this process works. If the specialist who examines bloodstain is not trained properly then outcome of the theory could be false or misconstrued. A person who is legally certified to analyze blood stain patterns must possess “characteristics of stain pattern, proper identification and examination of blood pattern, precise documentation of blood pattern and stains” . One ...

Chapter 4: Prokaryotic Cells and microorganisms

This chapter looks at the morphology, and pathogenic mechanisms of prokaryotic cells and microorganisms respectively. It discusses in detail the structural components, functions of the different organelles of prokaryotic cells as well as feeding and survival mechanisms of prokaryotic microorganisms.

Prokaryotic cells have a specialized cellular structure. Prokaryotes are unicellular and their cells have no cellular organelles, that is, a membrane does not surround their nucleus. These cells contain intricate phospholipids, sterols, and sphingolipids. They are typically 1-5µm in diameter .

Their outer surface is covered by glycocalyx, which is a gel like cellular envelope that provides prokaryotic cells with protection against ...

Mountain pine beetle (MPB) (Dendroctonus ponderosae Hopkins) is a bark beetle that lays eggs within the vascular system of the mature trees of lodgepole pine (Pinus contorta). First known epidemic infestation was recorded in 1994 in the forests of British Columbia (BC). Prior to 1994, the infestation was negligible, owing to the fact that extreme low temperatures of winter killed the beetle, thereby curbing the problem. In the past two decades, however, due to global warming the winter temperatures are not low enough to kill the beetle. This has escalated the problem of deforestation and thereby has unbalanced the ...

Aim: To separate Green Fluorescent Protein (GFP) using poly-acrylamide gel electrophoresis (PAGE).

Introduction: Bioluminescence observed in many marine organisms is due to a protein called as Green Fluorescent Protein (GFP). Since its discovery, GFP has been useful in biotechnology due to its versatility and ease of transformation in E. coli (Chalfie et al., 1994). GFP is a 26.87 kilodaltons (kD) protein composed of 239 amino acids. It has a barrel structure with a fluorescent chromophore embedded deep in the interior of the protein. The chromophores in GFP are excited at a wavelength of 395 nm, and when the electrons of chromophores drop to a lower energy state they emit fluorescent light of 509 nm (Ormo et al., ...

Microbiology is the study of microorganisms. Microorganisms are those organisms that are less than a millimeter in size and require the help of a microscope to view them. Microorganisms can be unicellular or multicellular but have very simple cell structure, unlike the highly developed, complex organisms such as larger animals and humans. Microorganisms are classified based on criteria such as their cell structure, pattern of growth, nutrition, reproduction method, etc. However, microbial classification is a challenging task due to the dual nature of some microorganisms that exhibit both animal and plant like characteristics. Some microorganisms are photosynthetic like plants but they also ...

Identification of mystery microbe based on its morphology and biochemical properties

The experiment was conducted to detect the species of a given mystery microbe. The microbe was subjected to a battery of tests in order to identify its physical and biochemical characteristics. Based on the tests, different possible microbe species were eliminated to finally arrive at the right conclusion. The possible microbes included Escherichia coli, Providencia alcalifaciens, Enterobacter aerogenes, Staphylococcus epidermidis, Klebsiella pneumonia, Micrococcus luteus, Serratia marcescens, Mycobacterium smegmatis, Sarcina aurantiaca, Pseudomonas aeruginosa and Bacillus licheniformis. The first test conducted was to detect the Gram staining properties of the given microbe. This helped in identifying whether the given microbe had a thin ...

Project Title

At about 2:30 in the morning of September 20, 2014, Detective James Reid called in for assistance in processing a possible murder case in No. 306, White Ridge Apartments, located in the corner of Strange Valley and Liberty Streets. The undersigned investigator arrived at the scene approximately 20 minutes later. No. 306, White Ridge Apartments is a single bedroom apartment and its current occupant was identified by the building management as Chuck Matthis, a single, white male working as IT technician at the Silver-Toned Company. The light at the lobby to the apartment was busted and the main door of the ...

{Student’s Name} {} {Course Name} {Date}

King Lear is one of Shakespeare’s most revered tragic plays of all time and even deemed by many critics as the most tragic. The play’s tragic end was so harshly condemned by the audiences that other writers wrote alternative “happier” endings that were even played on stage for quite some time. However, the play’s true essence lies in the original end and this was realized by critics and audiences alike and since then has been performed worldwide in its original form (Shakespeare and Halio). ...

Sustainability Issues for Textiles

The textile sustainability issues borders the manufacture of fabric from manufactured and natural fibers. As usual, this involves processes such as blending, dyeing, weaving, knitting or stripping. In a broader context, sustainability in the textile industry focuses on social issues such as working conditions, workers safety and health. However, the environmental aspects in the production process as core elements of sustainability deserve urgent attention. The main environmental issues regarding textile sustainability comprises of chemical use, water use, pollution, and energy use. In essence, materials such as wool have strong resilience and utility, thus makes them friendly to the environment as opposed ...

Immunohistochemistry

Introduction The Human Papilloma virus (HPV) is an extremely common group of viruses that are found worldwide. There are more than 100 types of HPV worldwide. 13 types of HPV are associated with cancer. They are also termed as high risk type. (WHO, 2013). HPV is transmitted through sexual contact. More than 90% of cases are infected shortly after sexual activity. Cervical cancer is associated with HPV infection that are acquired through sexual contact. 70% of cervical cancer cases are caused by two types of HPV. HPV 16 and HPV 18 are the two strains associated with cervical ...

The process of identifying an organism involves the determination of where an isolate or an organism needs to be placed within a group of organisms that are already known. The process is usually done to conform to a given scheme of classification. Most of the microorganisms that may be found in the environment require to be identified aiming at identifying the different species that may be found in a given environment. The identification enables physicists and health workers to associated different sources with different organisms especially those that cause diseases. Organism classification may also help in the diagnosis of different disease by ...

Poem and Poem Analysis on Femininity

[Client’s Name] [Client’s University]

PART 1: Creative Writing

The Beautiful Thing Object, that’s what It is Dismembered, molded – plastic Beautifuluntil It isn’t The existence of a stain is Its sin It is a docile body But It made itself ideal Choice however Oh how funny! Oh the irony! Do you know the story? It thinks it made the decision! Far from the truth Delusional object, that thing is The thing is cold to the touch As sinister as the carefully painted lips But who painted ...

Differentiation of white blood cells by light microscopy

Most mammals have blood consisting of three types of blood cells, Red blood cells (Erythrocytes), White blood cells (Leukocytes) and Platelets (Thrombocyte). These are collectively termed as hematocyte, which simply means blood cell. These cells constitute to around 40% to 45% of the blood volume. The remaining consist of Plasma, which is referred to the liquid part of blood. The total volume of blood can be determined by centrifugation or flow cytometry. The 45% of blood component (blood cells) is termed as hematocrit. Another important component of blood is hemoglobin, which gives blood its red color. It is ...

Microbiology Lab

Abstract This laboratory report tries to recognize an unknown bacterial species. The Gram stain disclosed bacteria to be both gram-negative and positive and via microscopy it confirmed to be rod and cocci respectively. Now two different bacteria were on the agar. One was a coliform and other a non coliform. The bacteria were exposed to all essential microbiological techniques employed to recognize an unknown bacterial species. The unknown comprised about 1% of the total bacterial flora of humans and even most of the warm-blooded animals. The bacteria are the most extensively studied prokaryotic organism, and an essential species in the ...

Purpose

Materials and Methods Gram Stain: the test was done to differentiate Gram-positive from Gram-negative organisms. A drop of the unknown 300 was suspended on a slide and the culture spread to an oven thin film. The culture was air dried and fixed over a gentle flame. Crystal violet stain was added over the culture and allowed to stand for 60 seconds. The stain was poured off and rinsed gently. Iodine solution was added on the smear, allowed to stand for 60 seconds and the stain poured off. The slide was rinsed with running water, and a few drops of decolorizer solution ...

MS WORD PART 1

This article is intents to highlight the unfolding events around China’s politician Bo Xilai who was sentenced to a life term in prison due to his alleged involvement in grand corruption. The accused was charge on the following counts; abuse of power, bribery and embezzlement having been a former party chief. The downfall of Bo Xilai come a moment when his wife had been convicted for alleged involvement in the murder of a British businessman Neil Heywood. Perhaps, this events are considered the biggest shakeup to have ever occurred to the china’s political elites group. The decision to rule a life ...

Derek Parker Royal’s article “Plotting the Frames of Subjectivity: Identity, Death, and Narrative” review of Philip Roth’s The Human Stain, focuses on the book’s protagonist, first person narrator, Nathan Zuckerman. Zuckerman’s identity as a writer of published works, American Pastoral and I Married a Communist is the site of inquiry in Royal’s commentary on Roth’s character development, by way of Zuckerman’s artistic process and textual production. Royal’s review looks at how Roth’s portrayal of Zuckerman also captures the complexity and oft compelling confusion of the protagonist as he attempts to introduce ...

UNKNOWN #16

SUMMER 2013 INTRODUCTION Purpose The purpose of the lab is to separate characterize and identify microbes from unknown mixed culture using the morphological and biochemical methods.

Theory

The process of identifying bacteria is a systematic and careful process which uses various techniques to narrow down the bacteria types which are present in an unknown culture of bacteria. This process produces advantages for many factors of research of microorganisms and assists in the proper treatment of patients (Aneja 10). Several tests were performed to give the fermentation abilities, availability of specific enzymes and specific biochemical reactions.

MATERIALS AND METHODS

The experiment started ...

Tuberculosis is one of the oldest diseases known to cause medical illness in humans, and is the second most common cause of death due to infection worldwide (Riley, 2013). The bacteria mycobacterium tuberculosis (M. Tuberculosis) causes the disease; and while it is widely known that it affects the lungs of the individual, other organ systems may be affected in nearly one-third of cases (Harrison’s, 2008). Proper treatment in drug-susceptible strains leads to a cure; however, drug resistant strains or improper treatment has a 5-year survival rate between 50-65% of cases (Harrison’s, 2008). Transmission is through respiratory droplets passed from ...

Latent Fingerprinting and its Reliability as Forensic Evidence

Introduction The discovery and subsequent improvement in forensic science has been a road marred with many challenges. However, despite these challenges, its acceptance as a rudimentary source of evidence in the judicial system is fascinating. Nonetheless, there are copious number of skeptics who argue that the science is based on probability; thus does not fit to be termed as an actual science. (1) Among the source of their skepticism is in evidence collection. In this work, there is an exploration of fingerprint collection on diverse surfaces.

Ways of Collecting Fingerprint Evidence from different Surfaces

Fingerprints can be identified from several surfaces, which include the porous, non-porous, and semi-porous ...

Part 1

1. 1. Eye piece 5.Arm 2. Objective lens 6.Coarse adjustment knob 3. Stage 7.fine adjustment 4. Base 2. What is the procedure (step by step) which you followed to do gram staining? (20 points) 1. Obtain a tissue sample for the gram stain. 2. Two or three drops of the sample were placed on the glass slide. 3. Use the electric slide warmer, heat the smear. Alternatively, this can be done by quickly passing it over a flame. Although should be very quick to avoid distortion. 4. Add crystal violet to the ...

Component One

The Data Quality Objectives (DQO Process) is a planning process that has been developed by the United States Environmental Protection Agency. This particular planning process is used when environmental information or data is used to make a choice between two specific alternatives or to make a derivation of contamination estimate. The process is also used in the development of acceptance and performance criteria that find use in the clarification of study objectives, defining of appropriate data type and the specification of potential errors of decisions that are then used as the basis for the establishment of the quantity and the quality of ...

So what do you expect to find in a play written hundreds of years ago but is still read with unrelenting enthusiasm and interest as never before? What is that make this possible? Can you really tell the best way of presenting a play in which a character, cheered by his over ambitious wife, kills people mercilessly in order to gain power and safeguard it? These are some of the questions that you get answered as you read Macbeth, a Shakespeare’s play .In my analysis of this play, I am going to discuss some major characters ,themes and stylistic devices that I ...

of HT22 Cells derived from Mouse Hippocampus

Sections I and II

Biology Section I 1. Introduction Fluorescent protein development research has gained increased knowledge about the range in the color palette available from the Aequorea Victoria jellyfish. The wavelength emissions in the blue to yellow range are now better understood. [1] Another area that has seen a great improvement in knowledge is the understanding of emissions of the monomeric fluorescent protein emissions in the orange to far-red spectral region. [1] The spectral region of fluorescent in the orange to far-red is derived appropriate reef coral species. [1] This area of research ...

Introduction

Bacillus Subtilis are the organisms that are aerobic, Gram Positive, spore-forming belonging to the Bacillus genus. The organisms are together with other species that are closely related play a crucial role in disease, food poisoning as well as food spoilage. Lack of methods that are standardized for the identification of the organisms whether coming from the food or environment has made the tests to be difficult. Use of morphological as well as physiological tests have offered the best ways to identify organisms in the laboratory although there are inconsistent and lack of reliance in these methods. Identification of an ...

Introduction

The history of marijuana in the twentieth century has been a slow process for its social acceptance. This process of acceptance still continued in twenty-first century. If we look into the history, In the 1936 a movie "Reefer Madness," shows marijuana smokers, they were portrayed as morally depraved fiends. In 1950s, marijuana was considered as a dangerous drug, people assumes that marijuana was a stepping stone towards the use of heroin and more dangerous drugs. In 1979, In a pole by CBS News, New York showed that27 percent of Americans were in favored legalization of marijuana by taxing it. Slowly but consistently, the social ...

A product strategy is the final vision of a product that helps determine the roadmap that the product will follow to achieve the eventual purposes set for that product. A product strategy enables an organisation to have a defined market as the focal point. Product strategy helps organisations define what they are selling, what value consumers derive from their products, how the products are priced and the distribution of that product to consumers (Susan & Nikolaos, 2000). OMO is a detergent manufactured by Unilever Company. Unilever is employing its product strategy to ensure that it maintains its market share. In their product ...

THE MICROSCOPE

The fundamental objectives of this report are to learn how to: - handle and care about the microscope - Identify various parts of the microscope such as the eye piece, arm, coarse adjustment knob, fine adjustment knob, base, nose piece, objective lenses, stage condenser, and Iris diagram. - Define various terminologies used while using the microscope such as the total magnification, resolving power, working distance, parfocal and real and virtual images. - And finally to observe stained slides of bacteria or the fungi.

RESULTS

Concerning how to care about the microscope, the following should be taken ...

Different parts of a car are made of different material. The choice of material to make different parts of a motor vehicle is influenced by factors like amount of stress handled by the part, cost of material compared to other alternatives and purpose of the part e.g. decoration etc. These are some of the factors the determine type of material a motor vehicle part is made of. An example of material and part on a car made using the material is given below. (A) Ferrous alloy 1.0 Part made of ferrous alloy

Engine components e.g. bolts and nuts, engine ducts and ...

Philip Roth's 2000 novel The Human Stain deals with many controversial and heated topics, such as racism, divorce, political controversy and sensationalism. The way in which the book's central character, Coleman Silk, is destroyed through seemingly harmless acts is a powerful statement on the nature of racial politics. This is made even more complex by the eventual reveal of Silk's true secret, placing his predicament in a wholly new light. The effects of these events affect both Silk and many other characters in the novel in dramatic ways. In this essay, the characters of Silk, narrator Nathan Zuckerman and Silk's secret ...

The Human Stain by Phillip Roth is a novel that depicts a series of disgraceful events and dissatisfactions in a manner that it makes a reader highly inquisitive to read the novel further with a larger intensity into it. Roth is a maestro of such works and rarely fails to surprise readers with an array of practical as well as imaginary metaphors. In the human stain, Roth has chosen the characters very smartly and depicted the incidents on a good plot. It is a novel that depicts sex, desires and several other hidden tendencies of human beings to deceive and how people exploit others. The story, ...

Weather: Clear, warm, temp at 86 F (outside) and 68 F (inside)

Time: Arrived on scene at 2058hrs

This was in response to an emergency phone call at 2055 hrs, made by a female who was hysterical but managed to give precise location of incident. On arrival at house, I found an early twenties white female lying on the living room couch and she seemed traumatized and confused. I quickly assume she is the caller and it was later confirmed that indeed she was Miss Jaen Daugh. She was assisted from the apartment by my colleagues and taken to the ...

Introduction

Ice cream, a milk-based good, is an excellent medium for microbial growth owing to high nutrient value, virtually neutral pH value (~pH 6–7), and elongated storage interlude of ice cream. The components of ice cream may be of a diversity of amalgamations of milk, cream, condensed milk, dried milk, colouring materials, flavours, fruits, nuts, sweetening agents, eggs, and eggs products, and stabilizers. Any of these could add to microorganisms’ growth and influence the quality of the goods as evaluated by its bacterial load or the presence of different explicit bacteria species. Time reliant heating throughout the ice cream creation decreases ...