The purpose of research
The experiment essentially investigates the association between endoplasmic reticulum and mitochondria.
Mitochondrion is a cell organelle, which moves along actin filaments. They are also calcium storage sites in the cell. The positioning and movement is influenced by membrane-cytoskeleton interactions. Reports suggest that the release of calcium from mitochondria and endoplasmic reticulum is influenced by structural changes in microtubules.
1. YFP-HDEL/atp-mito-CFP line.
2. 2μM Latrunculin-B
3. Ethanol control.
4. Arabidopsis Sail359/Aequroin line expressing atp-GFP-Aequorin.
Actin depolymerization affects mitochondrial clustering and movements. In the experiment, YFP-HDEL/ atp-mito-CFP line was treated with 2μM lat-B for 3 hours and 24